Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Screening of Korean Traditional Prescriptions with Inhibitory Activity against Protein Tyrosine Phosphatase 1B and Analysis of Jakgamhwangsinbu-tang (芍甘黃辛附湯) Prescription

전통 처방의 Protein Tyrosine Phosphatase 1B 저해 활성 검색 및 작감황신부탕(芍甘黃辛附湯) 처방 분석

  • Lee, Woojung (Natural Medicine Center, KIST Gangneung Institute) ;
  • Kim, Hyun Jung (College of Pharmacy and Natural Medicine Research Institute, Mokpo National University) ;
  • Moon, Hong Seop (College of Pharmacy and Natural Medicine Research Institute, Mokpo National University) ;
  • Kim, Su-Nam (Natural Medicine Center, KIST Gangneung Institute) ;
  • Yoon, Goo (College of Pharmacy and Natural Medicine Research Institute, Mokpo National University)
  • 이우정 (한국과학기술연구원 천연의약센터) ;
  • 김현정 (목포대학교 약학대학) ;
  • 문홍섭 (목포대학교 약학대학) ;
  • 김수남 (한국과학기술연구원 천연의약센터) ;
  • 윤구 (목포대학교 약학대학)
  • Received : 2013.03.22
  • Accepted : 2013.05.07
  • Published : 2013.06.30
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Abstract

In order to search for protein tyrosine phosphatase 1B (PTP1B) inhibitors as therapy of type 2 diabetes and obesity from Korean traditional prescriptions, we selected 58 traditional prescriptions based on a review of the Korean traditional medicine books. The hot water extracts of Korean traditional prescriptions were screened for the inhibitory activity against PTP1B. Among the tested extracts, water extracts of Jakgamhwangsinbu-tang, Seonbanghwalmyung-eum, and Takreeonjoong-tang showed relatively good inhibitory activity against PTP1B at the concentration of $30{\mu}g/ml$. Additionally, we evaluated PTP1B inhibitory effect for each herbal ingredient and composition in Jakgamhwangsinbu-tang (芍甘黃辛附湯). Of the tested ingredients from this herbal medicine, water extracts of Paeoniae Radix rubra and Rhei Rhizoma, and ethanol extracts of Paeoniae Radix alba, Rhei Rhizoma, Asiasari Radix, and Aconiti Tuber showed good PTP1B inhibitory effect. Herbal compositions composed of these active herbal ingredients exhibited significant activity for PTP1B inhibition over 70% at $7.5{\mu}g/ml$.

Keywords

References

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