Radical Scavenging Activities of Fruits of Crataegus pinnatifida BUNGE Major. from Korea

  • Choi, You Jin (Plant Resource Research Institute, Duksung Women's University) ;
  • Hwang, Keum Hee (Plant Resource Research Institute, Duksung Women's University)
  • Received : 2013.04.16
  • Accepted : 2013.06.02
  • Published : 2013.06.30

Abstract

Screenings of potential antioxidant activities of Crataegus pinnatifida BUNGE Major. fruits extracted 80% methanol were performed using four antioxidant assays. Significant differences were observed both in total phenolic contents (TPC) and total flavonoid contents (TFC), DPPH radical scavenging activity, nitric oxide scavenging activity, ABTS radical scavenging assay, and reducing power assay. The total polyphenol content and total flavonoid content in the extract were measured to be $224.4{\pm}0.52$ mg GAE/100 g and $12{\pm}0.25$ mg QE/100 g, respectively. When the tested concentration was $500{\mu}g/mL$, DPPH and ABTS radical-scavenging activities of methanolic extracts were 84.15% and 88.8%, respectively. The reducing power and nitric oxide scavenging activity were increased at the manner of dose-dependently. These results suggest that methanolic extracts of Crataegus pinnatifida Bge. fruits possess excellent radical scavenging activities and may serve as a potential source of natural antioxidant.

Keywords

References

  1. Amin, A. and Yazdanparst, R., Antioxidant and free radical-scavenging potential of Achillea santolina extracts. Food Chem. 104, 21-29 (2007). https://doi.org/10.1016/j.foodchem.2006.10.066
  2. Ammon, H.P.T. and Handel, M., Crataegus, Toxicology, and pharmacology II. Pharmacodynamics and pharmacokinetics. Planta Medica, 43, 105-120 (1981b). https://doi.org/10.1055/s-2007-971487
  3. Ammon, H.P.T. and Handel, M., Crataegus, Toxicology and pharmacology I. Toxicity. Planta Medica, 43, 105-120 (1981a). https://doi.org/10.1055/s-2007-971487
  4. Ammon, H.P.T. and Handel, M., Crataegus, Toxicology and pharmacology III. Pharmacodynamics and pharmacokinetics. Planta Medica, 43, 105-120 (1981c). https://doi.org/10.1055/s-2007-971487
  5. Beckman, J.S. and Koppenol, W.H., Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and ugly. American Journal of physiology-Cell Physiology. 271, C1424-C1437 (1996).
  6. Braca, A., De, Tommasi, N., Di, Bari, L,, Pizza, C., Politi, M., and Morelli, I., Antioxidant pronciples from Bauhinia tarapotensis. Journal of Natural Products. 64, 892-895 (2001). https://doi.org/10.1021/np0100845
  7. Cai, Y., Luo, Q., Sun, M., and Corke, H., Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74, 2157-84 (2004). https://doi.org/10.1016/j.lfs.2003.09.047
  8. Chang, Q., Zuo, Z., Chow, M.S.S., and Ho, W.K.K., Effect of storage temperature on phenolics stability in hawthorn (Crataegus pinnatifida var. major) fruits and a hawthorn drink. Food Chemistry. 98, 426-430 (2006). https://doi.org/10.1016/j.foodchem.2005.06.015
  9. Choe, S.Y. and Yang, K.H., Toxicological studies of antitoxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Korean J Food Sci Technol, 14, 283-288 (1982).
  10. Di, M.V. and Esposito, E., Biochemical and therapeutic effects of antioxidants in the treatment of Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Curr. Drug Targets CNS Neurol. Disord. 2, 95-107 (2003). https://doi.org/10.2174/1568007033482959
  11. Duh, P.D., Antioxidant activity of burdock (Arctium lappa Linne): Its scavenging effect on free radical and active oxygen. J. Am. Oil Chem. Soc. 75, 455-461 (1998). https://doi.org/10.1007/s11746-998-0248-8
  12. Frishman, W.H., Beravol, P., and Carosella, C., Alternative and complementary makedicine for preventing and treating cardiovascular disease. Disease-a-Month. 55, 121-192 (2009). https://doi.org/10.1016/j.disamonth.2008.12.002
  13. Frishman, W.H., Sinatra, S.T., and Moizuddin, M., The use of herbs for treating cardiovascular disease. Seminars in Integrative Medicine. 2, 23-35 (2004). https://doi.org/10.1016/j.sigm.2004.05.002
  14. Gerber, M., Boutron-Ruault, M.C., Hercberg, S., Riboli, E., Scalbert, A., and Siess, M.H., Food and cancer: State of the art about the protective effect of fruits and vegetables. Bull. Cancer, 89, 293-312 (2002).
  15. Halliwell, B. and Gutteridge, J.M.C., Free radicals in biology and medicine. Oxford University Press. Oxford, UK, pp. 218-313, 1985.
  16. Jacob, R., Three eras of vitamin C discovery. Subell biochem. 25, 1-16 (1996).
  17. Jayaprakasha, G.K. and Rao, L.J., Phenolic constituents from lichen Parmotrema stuppeum (Nyl.) Hale and their antioxidant activity. Zeitschrift fur Naturforschung. 56, 1018-1022 (2000).
  18. Kang, S., Kim, D., Lee, B.H., Kim, M.R., Chiang, M., and Hong, J., Antioxidanr properties and cytotoxic effects of fractions from glasswort (Salicornia herbacea) seed extracts on human intestinal ccells. Food Sci. Biotechnol. 20, 115-122 (2011). https://doi.org/10.1007/s10068-011-0016-7
  19. Kim, Y.J. and Son, D.Y., Antioxidant effects of solvent extracts from the dried jujube (Zizyphus jujube) sarcocarp, seed, and leaf via sonication. Food Sci. Biotechnol. 20, 167-173 (2011). https://doi.org/10.1007/s10068-011-0023-8
  20. Kitts, D.D., Wijewickreme, A.N., and Hu, C., Antioxidant properties of a North American ginseng extract. Molecular and Cell Biochemistry. 203, 1-10 (2000). https://doi.org/10.1023/A:1007078414639
  21. Knight, J., Free radicals: their history and current status in aging and disease. Ann Clin Lab Sci, 28, 331-346 (1988).
  22. Lee, H.J. and Koh, K.H., Antioxidant and free radical scavenging activities of Korean wine. Food Sci. Biotechnol. 5, 566-571 (2001).
  23. Lemanska, K., Szymusiak, H., Tyrakowska, B., Zielinski, R., Soffer AEMF, and Rietjiens I.M.C.M., The influence of pH on the antioxidant properties and the mechanism of antioxidant action of hydroxyflavones. Free Radical Biology and Medicine. 31, 869-881 (2001). https://doi.org/10.1016/S0891-5849(01)00638-4
  24. Long, S.R., Carey, R.A., Crofoot, K.M., Proteau, P.J., and Filtz, T.M., Effect of hawthorn (Crataegus oxycantha) crude extract and chromatographic fractions on multiple activities in a cultured cardiomyocyte assay. Phytomedicine. 13, 643-650 (2006). https://doi.org/10.1016/j.phymed.2006.01.005
  25. Mahfuz, E., Omer, I., Ibeahim, T., and Nuri, T., Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J. Food Compos. Anal. 20, 337-345 (2007). https://doi.org/10.1016/j.jfca.2006.07.003
  26. Marcocci, L., Maguire, J.J., Droy-Lefaix, M.T., and Packer, L., The nitric oxidae-scavenging properties of Ginkgo biloba extract EGb 761. Biochem Biophys Res Commun. 201, 748-755 (1994). https://doi.org/10.1006/bbrc.1994.1764
  27. Meri, S., Kanner, J., Akiri, B., and Hadas, S.P., Determination and involvement of aqueous reducing compounds in oxidative defence systems of various senescening leaves. J Agric Food Chem 43, 1813-1819 (1995). https://doi.org/10.1021/jf00055a012
  28. Nogochi, C. and Nikki, E., Phenolic antioxidants: A rationale for design and evaluation of novel antioxidant druge for atherosclerosis. Free Radicals in Biology and Medicine. 28, 1538-1546 (2000). https://doi.org/10.1016/S0891-5849(00)00256-2
  29. Ordonez, A.A.L., Gomez, J.D., Vattuone, M.A., and Isla M.I., Antioxidant activities of Sechium edule (jacq.) Swartz extracts. Food Chem. 97, 452-458 (2006). https://doi.org/10.1016/j.foodchem.2005.05.024
  30. Oyaizu, M., Studues on products of the browning reaction. Antioxidative activities of browning reaction products prepared from glucosamine. Japn. J. Nutr. 44, 307-315 (1986). https://doi.org/10.5264/eiyogakuzashi.44.307
  31. Pacher, P., Beckman, J.S., and Liaudet, L., Nitric oxide and peroxynitrite: In health and disease. Physiological Reviews. 87, 315-424 (1996).
  32. Perez-Jimenez, J., and Saura-Calixto, F., Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Research International. 39, 791-800 (2006). https://doi.org/10.1016/j.foodres.2006.02.003
  33. Pittle, M.H., Schmidt, K., and Ernst, E., Hawthorn extract for treating chronic heart failure: Meta-analysis of randomized trials, The American Journal of Medicine, 114, 665-674 (2003). https://doi.org/10.1016/S0002-9343(03)00131-1
  34. Prasad, K.N., Yang, B., Dong, X., Jiang, G., Zhang, H., Xie, H., and Jiang, Y., Flavonoid contents and antioxidant activities from Cinnamomum species. Innov. Food Sci. Emerg. 10, 627-632 (2009). https://doi.org/10.1016/j.ifset.2009.05.009
  35. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evens, C., Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med. 26, 1231-1237 (1999). https://doi.org/10.1016/S0891-5849(98)00315-3
  36. Serafini, M., Bellocco, R., Wolk, A., and Ekstrom, A.M., Total antioxidant potential of fruits and vegetables and risk of gastric cancer. Gastroenterology 123, 985-991 (2002). https://doi.org/10.1053/gast.2002.35957
  37. Shen, Q., Zhang, B., Xu, R., Wang, Y., Ding, X., and Li, P., Antioxidant activity in vitro of selenium-contained protein from the se-enriched Bifidobacterium animalis 01. Anaerobe, 16, 380-386 (2010). https://doi.org/10.1016/j.anaerobe.2010.06.006
  38. Shetty, K., Curtis, O.F., Levin, R.E., Witkowsky, R., and Ang, V., Prevention of vitrification associated with in vitro shoot cultire of oregano (Origanum vulgare) by Pseudomonas spp. J Plant Physiol. 147, 447-451 (1995). https://doi.org/10.1016/S0176-1617(11)82181-4
  39. Simic, M.G., Mechanisms of inhibition of free-radical processes in mutagenesis and carcinogenesis. Mut Res, 202, 377-386 (1988). https://doi.org/10.1016/0027-5107(88)90199-6
  40. Taga, M.S., Miller, E.E., and Pratt, D.E., Chia seeds as a source of natural lipid antioxidants. Journal of American oil chemist's society. 61, 928-931 (1984). https://doi.org/10.1007/BF02542169
  41. Yao, M., Ritchie, H.E., and Brown-Woodman, P.D., A reproductive screening test of hawthorn. Journal of Ethnopharmacology, 118, 127-132 (2008). https://doi.org/10.1016/j.jep.2008.03.020
  42. Zhang, Z.S., Chang, Q., Zhu, M., Huang, Y., Ho, W.K.K., and Chen, Z.Y., Characterization of antioxidants present in hawthorn fruits. The Journal of Nutritional Biochemistry, 12, 144-152 (2001). https://doi.org/10.1016/S0955-2863(00)00137-6
  43. Zhang, Z.S., Ho, W.K.K., Huang, Y., and Chen, Z.Y., Hypocholesterolemic activity of hawthorn fruit is mediated by regulation of cholesterol-7ahydroxylase and acyl CoA: Cholesterol acyltransferase. Food Research International, 35, 885-891 (2002). https://doi.org/10.1016/S0963-9969(02)00099-6
  44. Zhang, Z.S., Ho, W.K.K., Huang, Y., and Chen, Z.Y., Hypocholesterolemic activity of hawthorn fruit is mediated by regulation of cholesterol-$7{\alpha}$-hydroxylase and acyl CoA: Cholesterol acyltransferase. Food Research International, 35, 885-891 (2002). https://doi.org/10.1016/S0963-9969(02)00099-6