Antioxidant Activities of Extracts from Various Kimchi with Heat and Non-heat Treatment of Salt-Fermented Anchovies

가열 및 비가열 처리 액젓으로 제조한 김치 추출물의 항산화 활성

  • Kang, Hyun Woo (Department of Food Science and Technology, Pukyong National University) ;
  • Cho, Young-Jae (Department of Food Science and Technology, Pukyong National University)
  • Received : 2013.03.26
  • Accepted : 2013.04.17
  • Published : 2013.04.30

Abstract

The free radical scavenging activities of extracts from kimchi, as well as various antioxidant activities, such as 2,2-azinobis-( 3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferric reducing antioxidant power (FRAP) were examined. Specifically, the ABTS radical scavenging and FRAP activities of ethanolic extracts from kimchi (kimchi with non-treated salt fermented anchovy, treated non-heat salt fermented anchovy, treated heat salt fermented anchovy and commercial salt-fermented anchovy) were $0.749{\pm}0.26$, $0.895{\pm}0.15$, $0.758{\pm}0.12$, and $0.769{\pm}0.02$ mM Trolox eq./mg extract and $0.727{\pm}0.11$, $0.901{\pm}0.03$, $0.811{\pm}0.17$, and $0.843{\pm}0.16$ mM $FeSO_4$ eq./mg extract, respectively. In addition, the antioxidant activity of extract from kimchi was estimated in vivo. Mice were randomly divided into six groups (n=6), and treated with LPS for 10 days, which resulted in lower antioxidant capacities as well as increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) values in the blood serum. Furthermore, antioxidant enzymes (catalase and superoxide dismutase) in blood serum increased in the presence of the kimchi. The present results reveal antioxidant activities from kimchi and encourage their application for the food industry and cooking.

Keywords

References

  1. Aruoma OI (1994) Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol 62: 671-683.
  2. Benzie IFF, Strain JJ (1999) Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 299: 15-27. https://doi.org/10.1016/S0076-6879(99)99005-5
  3. Cha YS, Oh SH (2000) Investigation of ${\gamma}$-aminobutyric acid in Chinese cabbage and effects of the cabbage diets on lipid metabolism and liver function of rats administered with ethanol. J Korean Soc Food Sci Nutr 29: 500-505.
  4. Cheigh HS, Park KY (1994) Biochemical, microbiological, and nutritional aspects of kimchi (Korean fermented vegetable products). Crit Rev Food Sci Nutr 34: 175-203. https://doi.org/10.1080/10408399409527656
  5. Cho YJ, Ju IS, Chun SS, An BJ, Kim JH, Kim MU, Kwon OJ (2008) Screening of biological activities of extracts from Rhododendron mucronulatum Turcz. flowers. J Korean Soc Food Sci Nutr 37: 276-281. https://doi.org/10.3746/jkfn.2008.37.3.276
  6. Cho Y, Rhee HS (1979) A study on flavorous taste components in kimchis. Korean J Food Sci Technol 11: 26-31.
  7. Choi YS (1999) Antioxidative activity of mustard leaf kimchi with optional ingredients. J Korean Soc Food Sci Nutr 29: 1003-1008.
  8. Dantzer R, Bluthe RM, Gheusi G, Cremona S, Laye S, Parnet P, Kelley KW (1998) Molecular basis of sickness behavior. Annals NY Acad Sci 856: 127-134.
  9. Do SD, Lee YM, Chang HG (1993) The study on kinds and utilities of jeot-kal(fermented fish products). Korean J Soc Food Sci 9: 222-229.
  10. Gurpreet K, Sarwar A, Zoobi J, Kaleem J, Mohammad A (2006) Evaluation of antioxidant activity of Cassia siamea flowers. J Ethnopharmacol 108: 340-348. https://doi.org/10.1016/j.jep.2006.05.021
  11. Halliwell B (1990) How to characterize a biological antioxidant. Free Radic Res Commun 9: 1-13. https://doi.org/10.3109/10715769009148569
  12. Halliwell B, Gutteridge JMC, Cross CE (1992) Free radicals, antioxidants and human disease: where are we now? J Lab Clin Med 119: 598-620.
  13. Hur YM, Kim SH, Choi JW, Park KY (2000) Inhibition of tumor formation and changes in hepatic enzyme activities by kimchi extracts in Sarcoma-180 cell transplanted mice. J Food Sci Nutr 5: 48-53.
  14. Hwang JW (1999) The effect of solvent fractions of kimchi on plasma lipid concentration of rabbit fed cholesterol diet. J Korean Soc Food Sci Nutr 29: 204-210.
  15. Hyun YH, Koo BS, Song JE, Kim DS (2004) Food materials. Hyungseul, Daegu. pp 152-153.
  16. Jeon YS, Kye IS, Cheigh HS (1999) Changes of vitamin C and fermentation characteristics of kimchi on different cabbage variety and fermentation temperature. J Korean Soc Food Sci Nutr 28: 773-779.
  17. Kang EJ, Jeong ST, Lim BS, Jo JS (1999) Quality changes in winter Chinese cabbage with various storage methods. Korean J Postharvest Sci Technol 6: 173-178.
  18. Kang HW, Cho YJ (2013) Characteristics of salt fermented anchovies with heat treatment. J East Asian Soc Dietary Life 2: 92-97.
  19. Kim EM, Kim YJ, Jeong MK (2004) Preference and eating activities of Chinese for traditional Korean kimchi. J Korean Soc Food Sci Nutr 33: 1641-1645. https://doi.org/10.3746/jkfn.2004.33.10.1641
  20. Kim GH, Kang JK, Park HW (2000) Quality maintenance of minimally processed Chinese cabbage for kimchi preparation. J Kor Soc Food Sci Nutr 29: 218-223.
  21. Kim HK, Lee GD, Kwon JH, Kim KH (2005) Monitoring on extraction yields and functional properties of Braccia oleracea var. captia extracts. Food Sci Biotechnol 14: 836-840.
  22. Kim ID, Kang KS, Kwon RH, Yang JO, Lee JS, Ha BJ (2007) The effect of Rubus coreanum Miquel against lipopolysaccharide-induced oxidative stress and lipid metabolism. J Fd Hyg Safety 22: 213-217.
  23. Kim JS (2002) Strategies for kimchi. Food Distribution Research 19: 91-101.
  24. Kim SH, Kim JO (2002) A study on the strategy to promote export for Korean kimchi business to Japan. Collection of treatises, Mokwon University 42: 313-340.
  25. Kim SY, Ryu KS, Lee WC, Ku HO, Lee HS, Lee KR (1999) Hypoglycemic effect of mulberry leaves with anaerobic treatment in alloxan-induced diabetic mice. Korean J Pharmacogn 30: 123-129.
  26. Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeon BT (2011) Antioxidant activity and protective effects of extracts from Helianthus tuberosus L. leaves on t-BHP induced oxidative stress in chang cells. J Korean Soc Food Sci Nutr 40: 1525-1531.
  27. Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH (2011) Antioxidant activities of extracts from Ligustrum ovalifolium H. leaves. J Korean Soc Food Sci Nutr 40: 1642-1647.
  28. Kim YS, Rhee HS (1985) The changes of chlorophylls in blanched and fermented Chinese cabbage. Korean J Soc Food Sci 1: 27-32.
  29. Ko YT, Hwang JK, Baik IH (2004) Effects of jeotkal addition on quality of kimchi. Korean J Food Sci Technol 36: 123-128.
  30. Ku HS, Noh JS, Kim HJ, Cheigh HS, Song YO (2007) Antioxidant effects of sea tangle added Korean cabbage kimchi in vitro and in vivo. J Korean Soc Food Sci Nutr 36: 1497-1502.
  31. Lee MA, Choi HJ, Kang JS, Choi YW, Joo WH (2008) Antioxidant activities of the solvent extracts from Tetragonia tetragonioides. Journal of Life Science 18: 220-227. https://doi.org/10.5352/JLS.2008.18.2.220
  32. Lee SJ, Kim YS, Hwang JW, Kim EK, Moon SH, Jeon BT, Jeon YJ, Kim JM, Park PJ (2012) Purification and characterization of a novel antioxidative peptide from duck skin by-products that protects liver against oxidative damage. Food Research International 49: 285-295. https://doi.org/10.1016/j.foodres.2012.08.017
  33. Lee YO, Cheigh HS (1996) Antioxidant activity of various solvent extracts from freeze dried kimchi. Journal of Life Science 6: 66-71.
  34. Lim BO, Jeong YJ, Park MH, Kim JD, Hwang SJ, Yu BP (2007) Immunoregulatory effects of saengshik on DSS induced inflammatory bowel disease in mouse model system. J Korean Soc Food Sci Nutr 36: 32-42. https://doi.org/10.3746/jkfn.2007.36.1.032
  35. Park KW, Kim MZ (1985) Influence of cultivar and storage period on the quality of Chinese cabbage. J Korean Soc Hort Sci 26: 299-303.
  36. Penman A, Sanderson GR (1972) A method for the determination of uronic acid sequence in alginates. Carbohydrate Res 25: 273-282. https://doi.org/10.1016/S0008-6215(00)81637-7
  37. Rina Y. (1995) Effect of dietary hot red pepper powder on humoral immune response in rats. J Korean Soc Food Sci Nutr 24: 837-842.
  38. Ryu SH, Kwon MJ, Song YS, Moon GS (2003) Effect of kimchi diet on antioxidative system rat. Inje Journal 18: 475-488.
  39. Song YO (2004) The functional properties of kimchi for the health benefits. Food Industry & Nutrition 9: 27-33.
  40. Yoo EJ (2004) The cytotoxic, andtioxidative and antihypertensive activities in Dolsanleaf mustard and its kimchi. Ph D Dissertation Yeosu National University, Yeosu. pp 40-44.
  41. Yoon SS (1992) A Historical study of Korean traditional kimchi. Korean J Dietary Culture 6: 467-477.