폐기물 취급 업종에서 우점하는 미생물에 대한 평가

A Study of Dominant Microorganisms in Waste Handling Industries

  • 박해동 (한국산업안전보건공단 산업안전보건연구원) ;
  • 박현희 (한국산업안전보건공단 산업안전보건연구원)
  • Park, Hae Dong (Occupational Safety and Health Research Institute, KOSHA) ;
  • Park, Hyunhee (Occupational Safety and Health Research Institute, KOSHA)
  • 발행 : 2013.06.30

초록

Objectives: The objective of this study is to identify the composition of dominant microorganisms in waste handling industries. Methods: We collected airborne bacteria and fungi by agar plate impaction method in recyclable waste sorting industry, food recycling industry, landfill and incineration. Isolated dominant microorganisms were identified by VITEK system or morphological analysis. Results: We isolated totally 330 microorganisms in the process and outdoor. Bacillus was the most dominant genus in the all industries, and Sphingomonas, Acinetobacter, Staphylococcus, and Proteus was dominant bacterial genus. The dominant genus of fungi was Penicillium, Aspergillus, and Cladosporium in each industries. Enterobacter, Pseudomonas, Klebsiella, and Proteus was identified as the dominant gram negative bacteria. The ratio of bacteria being biosafety levels(class 1 or 2) was 58.3~77.8%. Conclusions: This study has investigated the dominant microorganisms in the waste handling industries. The genus of dominant microorganisms was similar among the industries but the composition was different. We used biosafety levels as qualitative method, but further studies are needed about specific process of qualitative evaluation methods.

키워드

참고문헌

  1. Domingo JL, Nadal M. Domestic waste composting facilities: A review of human health risks. Environment International 2009;35:382-389 https://doi.org/10.1016/j.envint.2008.07.004
  2. Dungan RS. Use of a culture-independent approach to characterize aerosolized bacteria near an open-freestall dairy operation. Environment International 2012;41:8-14 https://doi.org/10.1016/j.envint.2011.12.004
  3. Gomez AM, Yannarell AC, Sims GK, Cadavid-Restrepo G, Moreno Herrera CX. Characterization of bacterial diversity at different depths in the Moravia Hill landfill site at Medellin Colombia. Soil Biology & Biochemistry 2011;43:1275-1284 https://doi.org/10.1016/j.soilbio.2011.02.018
  4. Ha KC, Park DW, Kim SB, Kwon SJ, Jeong HJ, et al. Study on the validity of installation of washing facilities in the workplace(focus on sanitation work). KOSHA OSHRI 2010
  5. Han Y, Li L, Liu J. Characterization of the airborne bacteria community at different distances from the rotating brushes in a wastewater treatment plant by 16S rRNA gene clone libraries. Journal of Environmental Sciences 2013;25(1):5-15 https://doi.org/10.1016/S1001-0742(12)60018-7
  6. Health and Safety Executive. Health hazards in the waste and recycling industry. 2007.
  7. Just N, Kirychuk S, Gilbert Y, Letourneau V, Veillette M, et al. Bacterial diversity characterization of bioaerosols from cage-housed and floor-housed poultry operations. Environmental Research 2011;111:492-498 https://doi.org/10.1016/j.envres.2011.01.009
  8. Korean Agency for Technology and Standards. Bioindustry-pathogen list and its biosafety class-part2 bacteria(KS J 0050-2). 2010
  9. Kim KY, Jang GY, Park JB, Kim CN, Lee KJ. Field study of characteristics of airborne bacteria distributed in the regulated public facilities. J Korean Soc Occup Environ Hyg 2006a;16(1):1-10
  10. Kim KY, Lee CR, Kim CN, Won JU, Roh JH. Size-based characteristics of airborne bacteria and fungi distributed in the general hospital. J Korean Soc Occup Environ Hyg 2006b;16(2):101-109
  11. Korzeniewska E, Filipkowska Z, Gotkowska-Plachta A, Janczukowicz W, Dixon B, et al. Determination of emitted airborne microorganisms from a BIO-PAK wastewater treatment plant. Water research 2009;43:2841-2851 https://doi.org/10.1016/j.watres.2009.03.050
  12. Lavoie J, Dunkerley CJ, Kosatsky T, Dufresne A. Exposure to aerosolized bacteria and fungi among collectors of commercial, mixed residential, recyclable and compostable waste. Science of the Total Environment 2006;370:23-28 https://doi.org/10.1016/j.scitotenv.2006.05.016
  13. Ministry of Environment. Amount and disposal of national wastes. 2012
  14. Neumann HD, Balfanz J, Becker G, Lohmeyer M, Mathys W, et al. Bioaerosol exposure during refuse collection: results of field studies in the real-life situation. The Science of the Total Environment 2002;293:219-231 https://doi.org/10.1016/S0048-9697(02)00039-6
  15. O'Gorman CM, Fuller HT. Prevalence of culturable airborne spores of selected allergenic and pathogenic fungi in outdoor air. Atmospheric Environment 2008;42:4355-4368 https://doi.org/10.1016/j.atmosenv.2008.01.009
  16. Park HH, Park HD, Lee IS. A Study on the biological hazards exposure for waste handling industries in korea. J Korean Soc Occup Environ Hyg. 2012;22(4):265-275
  17. Park JH. Exposure assessment of biological agents in indoor environments. J. Env. Hlth. Sci. 2009;35(4): 239-248
  18. Poulsen OT, Breum NO, Ebbehoj N, Hansen AM, Ivens UI, et al. Sorting and recycling of domestic waste. Review of occupational health problems and their possible causes. The Science of the Total Environment 1995a;168:33-56 https://doi.org/10.1016/0048-9697(95)04521-2
  19. Paulsen OT, Breum NO, Ebbehoj N, Hansen AM, Ivens UI, et al. Collection of domestic waste. Review of occupational health problems and their possible causes. The Science of the Total Environment 1995b;170:1-19 https://doi.org/10.1016/0048-9697(95)04524-5
  20. Reinthaler FF, Haas D, Feierl G, Schlacher R, Pichler-Semmelrock FP, et al. Comparative investigations of airborne culturable microorganisms in selected waste treatment facilities and in neighbouring residential areas. Zbl. Hyg. Umweltmed 1998;202:1-17
  21. Vilavert L, Nadal M, Inza I, Figueras MJ, Domingo JL. Baseline levels of bioaerosols and volatile organic compounds around a municipal waste incinerator prior to the construction of a mechanical-biological treatment plant. Waste Management 2009;29:2454-2461 https://doi.org/10.1016/j.wasman.2009.03.012
  22. Wouters IM, Spaan S, Douwes J, Doekes G, Heederik D. Overview of personal occupational exposure levels to inhalable dust, Endotoxin, beta (1-3)-Glucan and fungal extracellular polysaccharides in the waste management chain. Ann Occup Hyg 2006;50(1):39-53