생체재료학회지 (Biomaterials Research)

  • 제17권3호
  • /
  • Pages.91-101
  • /
  • 2013
  • /
  • 1226-4601(pISSN)
  • /
  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지

미더덕껍질 혼합 하이드로콜로이드막의 물성분석 및 랫드의 피부창상 치료효능에 관한 연구

Property and Efficacy Analysis of Hydrocolloid Membrane Containing Styela Clava Tunic on the Wound Repair of Skin in SD Rats

  • 곽문화 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 고준 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 김지은 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 이영주 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 이승현 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 손홍주 (부산대학교 생명자원과학대학 생명환경화학과) ;
  • 이희섭 (부산대학교 생활환경대학 식품영양학과) ;
  • 정영진 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 황대연 (부산대학교 생명자원과학대학 바이오소재과학과)
  • Kwak, Moon Hwa (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Go, Jun (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Kim, Ji Eun (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Lee, Young Ju (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Lee, Seung Hyun (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Lee, Hee Seob (Department of Life Science & Environment Biochemistry, College of Natural Resources & Life Science, Pusan National University) ;
  • Son, Hong Joo (Department of Food Science & Nutrition, College of Human Ecology, Pusan National University) ;
  • Jung, Young Jin (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Hwang, Dae Youn (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University)
  • 발행 : 2013.09.01
⟨ 이전 논문 다음 논문 ⟩

초록

Aim of this study was to determine the effect of hydrocolloid membrane containing Styela clava tunic (HCM-SCT) on acute wound of skin. To achieve this, HCM-SCT was developed using the mixture dispersed SCT powder in hydrocolloid (HC), and an alteration on the histology and physiology of wound skin were observed in SD rats after the treatment of HCM-SCT for 11 days. Absorptiveness of HCM-SCT was rapidly increased by 430% at day 1 and then their level was maintained for a period of 4 days, while the surface roughness was higher in HCM-SCT than that of HCM. In animals study, SD rats treated with HCM-SCT did not induce any specific toxicity on the body weight, and the physical function of liver and kidney. The diameter of wound area was shorter in HCM-SCT treated group than these in Gauze (GZ) or HCM treated group. Especially, the group treated with HCM-SCT showed a significantly faster re-epithelization and decrease of epidermis thickness than those treated with HCM alone although a large amount of HC was remained in dermis region of both rats. Furthermore, the level of collagen and tumor growth factor-${\beta}1$ (TGF-${\beta}1$) were greatly increased in HCM-SCT treated group, while the level of angiogenesis-related proteins were significantly recovered to normal level in the same group. Therefore, these results indicate that HCM-SCT having high absorptiveness and tensile strength could accelerate the repair process of acute wound in SD rats through the stimulation of re-epithelization, collagen deposition and angiogenesis.

키워드

참고문헌

  1. N. Yoshiharu, L. Paul and C. Henri, "Crystal structure and hydrogen-bonding system in cellulose $I{\beta}$ from synchrotron X-ray and neutron fiber diffraction," J. Am. Chem. Soc., 124(31), 9074-9082 (2002). https://doi.org/10.1021/ja0257319
  2. D. M. Updegraff, "Semimicro determination of cellulose in biological materials," Anal. Biochem., 32(3), 420-424 (1969). https://doi.org/10.1016/S0003-2697(69)80009-6
  3. M. S. Kim and S. M. Kang, "The effect analysis on middle-aged women's facial wrinkles improvement of shaking neck exercise and collagen," Diet. J. Kor. Soc. Cosm., 18(6), 1223-1234 (2012).
  4. S. M. Kim, J. H. Lee, J. A. Jo, S. C. Lee and S. K. Lee, "Development of a bioactive cellulose membrane from sea squirt skin for bone regeneration a preliminary research," J. Kor. Oral Maxillofac. Surg., 31, 440-453 (2005).
  5. C. X. Xu, H. Jin, Y. S. Chung, J. Y. Shin, M. A. Woo, K. H. Lee, G. N. Palmos, B. D. Choi, and M. H. Cho, "Chondroitin sulfate extracted from the Styela clava tunic suppresses TNF-$\alpha$-induced expression of inflammatory factors, VCAM-1 and iNOS by blocking Akt/NF-${\kappa}B$ signal in JB6 cells," Cancer Lett., 264, 93-100 (2008). https://doi.org/10.1016/j.canlet.2008.01.022
  6. Y. J. Jung, "Properties of regenerated cellulose films prepared from the tunicate Styela clava," J. Kor. Fish. Soc., 41(4), 237-242 (2008).
  7. Y. J. Jung, B. J. An, D. Y. Hwang, H. D. Kim, S. M. Park, H. Cho and H. S. Kim, "Preparation and properties of regenerated cellulosic biomaterial made from Styela clava tunics," J. Biomed. Mater. Res., 12(2), 71-76 (2008).
  8. S. Y. Choi, E. Y. Choi, K. E. Lee, A. S. Song, S. H. Park and S. C. Lee, "Preparation and quality analysis of fish containing Styela clava tunic," J. Korean Soc. Food Sci. Nutr., 41(11), 1591-1595 (2012). https://doi.org/10.3746/jkfn.2012.41.11.1591
  9. S. K. Han, and H. J. You, "Wound coverage using advanced technology in Korea," J. Korean Med. Assoc., 54, 594-603 (2011). https://doi.org/10.5124/jkma.2011.54.6.594
  10. J. M. Porter, "A comparative investigation of re-epithelialization of split skin graft donor areas after application of hydrocolloid and alginate dressings," Br. J. Plast. Surg., 44, 333-337 (1991). https://doi.org/10.1016/0007-1226(91)90144-9
  11. D. Chakravarthy, N. Rodway, S. Schmidt, D. Smith, M. Evancho, and R. Sims, "Evaluation of three new hydrocolloid dressings: retention of dressing integrity and biodegradability of absorbent components attenuate inflammation," J. Biomed. Mater. Res., 28, 1165-1173 (1994). https://doi.org/10.1002/jbm.820281007
  12. O. G. Sohn, S. J. Sim, D. H. Lee, Y. K. Lee, J. H. Kim and D. J. Kim, "Preparation and swelling properties of poly (potassium acrylate-co-acrylamide) superabsorbent particles," Polymers, 28, 18-23 (2004)
  13. H. E. Thu, M. H. Zulfakar and S. F. Ng, "Alginate based bilayer hydrocolloid films as potential slow-release modern wound dressing," Int. J. Pharm. Sep., 15(434), 375-383 (2012).
  14. J. Kingkaew, N. Jatupaiboon, N. Sanchavanakit, P. Pavasant and M. Phisalaphong, "Biocompatibility and growth of human keratinocytes and fibroblasts on biosynthesized cellulose-chitosan film," J. Biomater. Sci. Polym. Ed., 21(8-9), 1009-1021 (2010). https://doi.org/10.1163/156856209X462763
  15. R. Dalgleish, "The human type 1 collagen mutation database," Nucleic Acid. Res., 25, 181-187 (1997). https://doi.org/10.1093/nar/25.1.181
  16. S. W. Lee, J. T. Oh, S. L. Shon and Y. S. Kim, "Monitoring of wound healing using polarization sensitive optical coherence tomography," J. Opt. Soc. Korea, 2, 17-18 (2005)
  17. M. Madlener, W. C. Parks, and S. Werner, "Matrix metalloproteinases (MMPs) and their physiological inhibitors (TIMPs) are differentially expressed during excisional skin wound repair," Exp. Cell Res., 242, 201-210 (1998). https://doi.org/10.1006/excr.1998.4049
  18. C. P. Chan, W. H. Lan, M. C. Chang, Y. J. Chen, W. C. Lan, H. H. Chang and J. H. Jeng, "Effects of TGF-${\beta}s$ on the growth, collagen synthesis and collagen lattice contraction of human dental pulp fibroblasts in vitro," Arch. Oral. Biol., 50(5), 469-479 (2005). https://doi.org/10.1016/j.archoralbio.2004.10.005
  19. P. M. Elias, "The skin barrier as an innate immune element," Semin. Immunopathol., 29, 3-14 (1997).
  20. A. Pivarcsi, I. Nagy, and L. Kemeny, "Innate immunity in the skin: how keratinocytes fight against pathogens," Curr. Immunol. Rev., 1, 24-42 (2005).
  21. R. Jayakumar, M. Prabaharan, P. T. Sudheesh Kumar, S. V. Nair, and H. Tamura, "Biomaterials based on chitin and chitosan in wound dressing applications," Biotechnol. Adv., 29, 322-337 (2011). https://doi.org/10.1016/j.biotechadv.2011.01.005
  22. M. S. Wilson and T. A. Wynn, "Pulmonary fibrosis: pathogenesis, etiology and regulation," Mucosal Immunol., 2, 103-121 (2009). https://doi.org/10.1038/mi.2008.85
  23. M. G. Jeschke, G. Sandmann, T. Schubert and D. Klein, "Effect of oxidized regenerated cellulose/collagen matrix on dermal and epidermal healing and growth factors in an acute wound," Wound Repair Regen., 13(3), 324-331 (2005). https://doi.org/10.1111/j.1067-1927.2005.130316.x
  24. D. O. Bates and R. O. Pritchard-Jones, "The role of vascular endothelial growth factor in wound healing," Int. J. Low. Extrem. Wounds, 2, 107-120 (2003). https://doi.org/10.1177/1534734603256626
  25. P. Bao, A. Kodra, M. Tomic-Canic, M. S. Golinko, H. P. Ehrlich, and H. Brem, "The role of vascular endothelial growth factor in wound healing," J. Surg. Res., 153, 347-358 (2009). https://doi.org/10.1016/j.jss.2008.04.023
  26. M. Karin and F. R. Greten, "NF-${\kappa}B$: Linking inflammation and immunity to cancer development and progression," Nat. Rev. Immunol., 5, 749-759 (2005). https://doi.org/10.1038/nri1703