Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
권호 표지

Preparation of Inverse Opal Scaffolds with Uniform Pore Sizes and Their Effect on Cell Proliferation

균일 기공 지지체 제조 및 기공 크기에 대한 세포 증식 효과

  • Oh, Myeong-Jin (Department of Biotechnology, The Catholic University of Korea) ;
  • Moon, Seung-Kwan (Department of Biotechnology, The Catholic University of Korea) ;
  • Kim, Sung-Eun (Department of Orthopaedic Surgery and Rare Diseases Institute, Korea University, Medical College, Guro Hospital) ;
  • Choi, Sung-Wook (Department of Biotechnology, The Catholic University of Korea)
  • 오명진 (가톨릭대학교 생명공학과) ;
  • 문승관 (가톨릭대학교 생명공학과) ;
  • 김성은 (고려대학교 구로병원) ;
  • 최성욱 (가톨릭대학교 생명공학과)
  • Published : 2013.09.01
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Abstract

Inverse opal scaffolds with different pore sizes were fabricated using uniform gelatin microspheres and used as a model system to validate the effects of pore size on cell proliferation. The poly(D,L-lactide-co-glycolide) inverse opal scaffolds exhibited well-arranged and uniform pore sizes (122, 293, and $462{\mu}m$). Two types of cells (NIH 3T3 and HepG2) were cultured on the inverse opal scaffolds with different pore sizes up to 28 days for proliferation assay. It was observed that NIH 3T3 cells proliferated faster in the scaffolds with pore sizes of $122{\mu}m$ compared to the other pore sizes, which is attributed to the large surface area of the scaffold. In the other hands, HepG2 cells were found to favor pore size of $462{\mu}m$ because they probably need enough exchange of nutrients and wastes for survival. These results reveal that the proliferation rate is affected by the pore size and each cell type has its own favorable pore size. The inverse opal scaffolds could potentially be used as a powerful platform for the studies on the optimization of pore size for tissue engineering and the interactions between cells or tissues and scaffolds.

Keywords

References

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