Korean Journal of Radiology

The Korean Society of Radiology (대한영상의학회)
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In Vitro and In Vivo Imaging of Prostate Cancer Angiogenesis Using Anti-Vascular Endothelial Growth Factor Receptor 2 Antibody-Conjugated Quantum Dot

  • Kwon, Haejin (Medicinal Chemistry Laboratory, Institute Pasteur Korea (IP-K)) ;
  • Lee, Jiyeon (Medicinal Chemistry Laboratory, Institute Pasteur Korea (IP-K)) ;
  • Song, Rita (Medicinal Chemistry Laboratory, Institute Pasteur Korea (IP-K)) ;
  • Hwang, Sung Il (Department of Radiology, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Institute of Radiation Medicine, Seoul National University Medical Research Center, Clinical Research Institute) ;
  • Lee, Junghan (Medicinal Chemistry Laboratory, Institute Pasteur Korea (IP-K)) ;
  • Kim, Young-Hwa (Department of Nuclear Medicine and Biomedical Sciences Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine) ;
  • Lee, Hak Jong (Department of Radiology, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Institute of Radiation Medicine, Seoul National University Medical Research Center, Clinical Research Institute)
  • Published : 2013.02.01
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Abstract

Objective: Authors aimed to determine the targeting ability of vascular endothelial growth factor receptor 2 (VEGFR2)- conjugated quantum dots (QDs) in vitro, and apply it for a xenograft prostate cancer mouse model. Materials and Methods: Conjugation reaction of QDs was performed by using the N-(3-dimethylaminopropyl)-N'- ethylcarbodiimide (EDC) and sulfo-(N-hydroxysulfosuccinimide) (Sulfo-NHS). The human umbilical vein cord endothelial cells (HUVECs) were incubated with QDs, conjugated with antiVGFR2, to see a specific binding in vitro. Fluorescent cell images were taken by a confocal microscope. The human prostate cancer cells (PC3) were injected to five nude mice on hind limbs to make the xenograft tumor model. QD-antiVEGFR2 antibody complex was injected into the tumor model and fluorescence measurements were performed at 1, 4, 9, 12, 15, and 24 hours after the injection. Results: The specific interaction between HUVECs and QD-antiVEGFR2 antibody was clearly shown in vitro. The in vivo fluorescence image disclosed that there was an increased signal of tumor, 12 hours after the injection of QDs. Conclusion: By showing endothelial cells binding with QDs-antiVEGFR2 antibodyand an experimental application of the antibody for VEGFR2 imaging in the prostate cancer xenograft mouse model, we suggests that the antibody-conjugated QDs can be a potential imaging tool for angiogenesis of the cancer.

Keywords

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