Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
권호 표지
DOI QR코드

DOI QR Code

Two Cases of Mycoplasma pneumoniae Pneumonia with A2063G Mutation in the 23S rRNA Gene in Siblings

  • Hong, Joo Hee (Department of Pediatrics, Wonju Medical Center) ;
  • Chun, Jin Kyong (Department of Pediatrics, Yonsei University Wonju College of Medicine) ;
  • Uh, Young (Department of Laboratory Medicine, Yonsei University Wonju College of Medicine) ;
  • Oh, Ki Jin (Department of Laboratory Medicine, Yonsei University Wonju College of Medicine) ;
  • Kim, Juwon (Department of Laboratory Medicine, Yonsei University Wonju College of Medicine) ;
  • Yoon, Kap Jun (Department of Laboratory Medicine, Yonsei University Wonju College of Medicine)
  • Published : 2013.01.02
⟨ Previous Next ⟩

Abstract

We describe 2 cases of pneumonia caused by the same macrolide-resistant Mycoplasma pneumoniae in siblings. M. pneumoniae was identified using real-time PCR. Direct sequence analysis of the 23S rRNA gene revealed a point mutation in V domain (A2063G) of the 23S rRNA gene.

Keywords

References

  1. Waites KB and Talkington DF. Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev 2004;17:697-728. https://doi.org/10.1128/CMR.17.4.697-728.2004
  2. Dorigo-Zetsma JW, Wilbrink B, van der Nat H, Bartelds AI, Heijnen ML, Dankert J. Results of molecular detection of Mycoplasma pneumoniae among patients with acute respiratory infection and in their household contacts reveals children as human reservoirs. J Infect Dis 2001;183: 675-8. https://doi.org/10.1086/318529
  3. Ferwerda A, Moll HA, de Groot R. Respiratory tract infections by Mycoplasma pneumoniae in children: a review of diagnostic and therapeutic measures. Eur J Pediatr 2001;160:483-91. https://doi.org/10.1007/s004310100775
  4. Nilsson AC, Bjorkman P, Persson K. Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection. BMC Microbiol 2008;8:93. https://doi.org/10.1186/1471-2180-8-93
  5. Touati A, Pereyre S, Bouziri A, Achour W, Khaldi A, Ben Jaballah N, et al. Prevalence of Mycoplasma pneumoniae-associated respiratory tract infections in hospitalized children: results of a 4-year prospective study in Tunis. Diagn Microbiol Infect Dis 2010;68:103-9. https://doi.org/10.1016/j.diagmicrobio.2010.05.010
  6. Morozumi M, Iwata S, Hasegawa K, Chiba N, Takayanagi R, Matsubara K, et al. Increased macrolide resistance of Mycoplasma pneumoniae in pediatric patients with community-acquired pneumonia. Antimicrob Agents Chemother 2008;52:348-50. https://doi.org/10.1128/AAC.00779-07
  7. Chironna M, Sallustio A, Esposito S, Perulli M, Chinellato I, Di Bari C, et al. Emergence of macrolide-resistant strains during an outbreak of Mycoplasma pneumoniae infections in children. J Antimicrob Chemother 2011;66:734-7. https://doi.org/10.1093/jac/dkr003
  8. Liu Y, Ye X, Zhang H, Xu X, Li W, Zhu D, et al. Characterization of macrolide resistance in Mycoplasma pneumoniae isolated from children in Shanghai, China. Diagn Microbiol Infect Dis 2010;67:355-8. https://doi.org/10.1016/j.diagmicrobio.2010.03.004
  9. Kim Y, Kim M, Yoo N, Park J, Lee Y, Byun S, et al. Development of realtime PCR assay for the detection of five major pneumonia pathogen panels. Clin Chem 2011;57(S):A161.
  10. Foy HM, Grayston JT, Kenny GE, Alexander ER, McMahan R. Epidemiology of Mycoplasma pneumoniae infection in families. JAMA 1966;197:859-66. https://doi.org/10.1001/jama.1966.03110110083019
  11. Cimolai N, Wensley D, Seear M, Thomas ET. Mycoplasma pneumoniae as a cofactor in severe respiratory infections. Clin Infect Dis 1995;21:1182-5. https://doi.org/10.1093/clinids/21.5.1182
  12. Morozumi M, Takahashi T, Ubukata K. Macrolide-resistant Mycoplasma pneumoniae: characteristics of isolates and clinical aspects of community-acquired pneumonia. J Infect Chemother 2010;16:78-86. https://doi.org/10.1007/s10156-009-0021-4
  13. Thurman KA, Walter ND, Schwartz SB, Mitchell SL, Dillon MT, Baughman AL, et al. Comparison of laboratory diagnostic procedures for detection of Mycoplasma pneumoniae in community outbreaks. Clin Infect Dis 2009;48:1244-9. https://doi.org/10.1086/597775
  14. Takahashi T, Morozumi M, Okada T, Chiba N, Asami R, Murayama SY, et al. Prolonged Mycoplasma pneumoniae infection in an elderly patient with community-acquired pneumonia. J Infect Chemother 2009;15:243-7. https://doi.org/10.1007/s10156-009-0692-x
  15. Niitu Y, Hasegawa S, Suetake T, Kubota H, Komatsu S, Horikawa M. Resistance of Mycoplasma pneumoniae to erythromycin and other antibiotics. J Pediatr 1970;76:438-43. https://doi.org/10.1016/S0022-3476(70)80485-1
  16. Lucier TS, Heitzman K, Liu SK, Hu PC. Transition mutations in the 23S rRNA of erythromycin-resistant isolates of Mycoplasma pneumoniae. Antimicrob Agents Chemother 1995;39:2770-3. https://doi.org/10.1128/AAC.39.12.2770
  17. Cao B, Zhao CJ, Yin YD, Zhao F, Song SF, Bai L, et al. High prevalence of macrolide resistance in Mycoplasma pneumoniae isolates from adult and adolescent patients with respiratory tract infection in China. Clin Infect Dis 2010;51:189-94. https://doi.org/10.1086/653535
  18. Lin C, Li S, Sun H, Zhao H, Feng Y, Cao L, et al. Nested PCR-linked capillary electrophoresis and single-strand conformation polymorphisms for detection of macrolide-resistant Mycoplasma pneumoniae in Beijing, China. J Clin Microbiol 2010;48:4567-72. https://doi.org/10.1128/JCM.00400-10
  19. Pereyre S, Guyot C, Renaudin H, Charron A, Bebear C, Bebear CM. In vitro selection and characterization of resistance to macrolides and related antibiotics in Mycoplasma pneumoniae. Antimicrob Agents Chemother 2004;48:460-5. https://doi.org/10.1128/AAC.48.2.460-465.2004
  20. Matsubara K, Morozumi M, Okada T, Matsushima T, Komiyama O, Shoji M, et al. A comparative clinical study of macrolide-sensitive and macrolide-resistant Mycoplasma pneumoniae infections in pediatric patients. J Infect Chemother 2009;15:380-3. https://doi.org/10.1007/s10156-009-0715-7
  21. Narita M, Tanaka H, Yamada S, Abe S, Ariga T, Sakiyama Y. Significant role of interleukin-8 in pathogenesis of pulmonary disease due to Mycoplasma pneumoniae infection. Clin Diagn Lab Immunol 2001;8:1028-30.
  22. Shinkai M, Foster GH, Rubin BK. Macrolide antibiotics modulate ERK phosphorylation and IL-8 and GM-CSF production by human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol 2006;290:L75-85.
  23. Abe S, Nakamura H, Inoue S, Takeda H, Saito H, Kato S, et al. Interleukin- 8 gene repression by clarithromycin is mediated by the activator protein-1 binding site in human bronchial epithelial cells. Am J Respir Cell Mol Biol 2000;22:51-60. https://doi.org/10.1165/ajrcmb.22.1.3400

Cited by

  1. Treating paediatric community‐acquired pneumonia in the era of antimicrobial resistance vol.102, pp.suppl465, 2013, https://doi.org/10.1111/apa.12503