The Korean journal of internal medicine

The Korean Association of Internal Medicine (대한내과학회)
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The dose of cyclophosphamide for treating paraquat-induced rat lung injury

  • Choi, Jae-Sung (Department of Internal Medicine, Soonchunhyang University Cheonan Hospital) ;
  • Jou, Sung-Shick (Department of Radiology, Soonchunhyang University Cheonan Hospital) ;
  • Oh, Mee-Hye (Department of Pathology, Soonchunhyang University Cheonan Hospital) ;
  • Kim, Young-Hee (Department of Microbiology, Soonchunhyang University College of Medicine) ;
  • Park, Min-Ju (Department of Microbiology, Soonchunhyang University College of Medicine) ;
  • Gil, Hyo-Wook (Department of Internal Medicine, Soonchunhyang University Cheonan Hospital) ;
  • Song, Ho-Yeon (Department of Microbiology, Soonchunhyang University College of Medicine) ;
  • Hong, Sae-Yong (Department of Internal Medicine, Soonchunhyang University Cheonan Hospital)
  • Published : 2013.07.01
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Abstract

Background/Aims: Cyclophosphamide (CP) is a promising treatment for severe cases of paraquat (PQ) poisoning. We investigated the effective dose of CP for mitigating PQ-induced lung injury. Methods: Adult male Sprague-Dawley rats were allocated into five groups: control, PQ (35 mg/kg, intraperitoneal injection), and PQ + CP (1.5, 15, or 30 mg/kg). The dimensions of lung lesions were determined using X-ray microtomography (micro-CT), and histological changes and cytokine levels were recorded. Results: The micro-CT results showed that 15 mg/kg CP was more effective than 1.5 mg/kg CP for treating PQ-induced lung injury. At a dose of 1.5 mg/kg, CP alleviated the histological evidence of inflammation and altered superoxide dismutase activity. Using 15 mg/kg CP reduced the elevated catalase activity and serum transforming growth factor (TGF)-${\beta}1$ level. Conclusions: A CP dose of > 15 mg/kg is effective for reducing the severity of PQ-induced lung injury as determined by histological and micro-CT tissue examination, possibly by modulating antioxidant enzyme and TGF-${\beta}1$ levels.

Keywords

References

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