Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of NaOCl treatment on in vitro germination of seeds of a rare endemic plant, Oreorchis coreana Finet

  • Bae, Kee-Hwa (Hongcheon Institute of Medicinal Herb) ;
  • Ko, Myoung Suk (Jeju Biodiversity Research Institute, Jeju Technopark) ;
  • Lee, Mi Hyun (Plant Quarantine Technology Center) ;
  • Kim, Nam Young (Department of Horticulture and Education, Korea National Arboretum) ;
  • Song, Jae Mo (Nature Environment Research Office of Gangwon Province) ;
  • Song, Gwanpil (Jeju Environment Research Institute, Rm 311, Bl Center, Jeju Tourism College)
  • Received : 2013.01.18
  • Accepted : 2013.01.31
  • Published : 2013.03.31
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Abstract

Oreorchis coreana Finet is threatened globally by over-collection from its natural habitats for horticultural purposes. Its rarity in nature makes this plant one of the most endangered species in Korea. In this study, we investigated the effects of sodium hypochlorite (NaOCl) on orchid seed viability and seed germination. An in vitro bioassay swelling test using immature seeds was compared with a standard chemical procedure using triphenyl tetrazolium chloride (TTC) to test seed viability. In general, the bioassay was more appropriate for estimating embryo viability after a prolonged pre-treatment (more than 1 h) in 1% NaOCl, a surface sterilant often used to enhance germination of seeds of terrestrial plants. Therefore, an efficient method for investigating in vitro swelling of immature seeds is urgently needed. We established a method for determining the viability and swelling of O. coreana seeds via in vitro examination of immature seeds. Treatment of immature seeds with 1% NaOCl for 10 min greatly enhanced the extent of swelling of immature zygote embryos when compared to untreated seeds. These data obtained here appear to be comparable to viability and swelling that occurs in O. coreana seeds via asymbiotic germination.

Keywords

References

  1. Anderson AB (1996) The re-introduction of Platanthera ciliaris in Canada. In: Allen C. (Ed.). Proceedings of the North American Native Terrestrial Orchids: Propagation and Production. North American Native Terrestrial Orchid Conference Maryland:73-76
  2. Ault JR, Blackmon WJ (1987) In vitro propagation of Ferocactus acanthodes (Cactaceae). Hort Science 22:126-127
  3. Bae KH, Choi YE (2011) Factors affecting fruit baring in natural habitat and in vitro culture of zygotic embryos of Cypripedium japonicum. Prog Ornament Plant 11:146-152
  4. Bae KH, Kim CH, Sun BY, Choi YE (2010) Structural changes of seed coats and stimulation of in vitro germination of fully mature seeds of Cypripedium macranthos Swartz by NaOCl pretreatment. Prog. Ornament. Plant 10:107-113
  5. Bae KH, Kwon HK, Choi YE (2009) In vitro germination and plantlet conversion from the culture of fully mature seeds of Cypripedium guttatum Swartz. Prog Ornament Plant 9:160-165
  6. Bewley, JD, Black M (1994) Seeds. Physiology of development and Germination. Second Edition New York, Prenum Press
  7. Dutra D, Kane ME, Richardson L (2009) Asymbiotic seed germination and in vitro seedling development of Cyrtopodium punctatum: a propagation protocol for an endangered Florida native orchid. Plant Cell Tiss Org Cult 96:235-243 https://doi.org/10.1007/s11240-008-9480-z
  8. Fast G (1982) European terrestrial orchids. Symbiotic and asymbiotic methods. In: Arditti J, ed. Orchid biologyreviews and perspectives II. Ithaca, NY: Comstock Publishing Associates 326-329
  9. Finet EA (1908) Oreorchis coreana sp. nov. Bull Soc Bot Fr 55:337
  10. Harvais G (1982) An improved culture medium for growing the orchid Cypripedium reginae axienically. Can J Bot 51: 327-332
  11. Harvais G, Hadley G (1967) The relation between host and endophyte in orchid mychorriza. New Phytol 66:205- 215 https://doi.org/10.1111/j.1469-8137.1967.tb05999.x
  12. IUCN (2009) IUCN Red List of treated species. Prepared by the IUCN Species Survival Commission. IUCN, Gland, Switzerland, http://www.iucnredlist.org
  13. Lakon, G (1949) The topographical tetrazolium method for determining the germinating capacity of seeds. Plant Physiol 24:389-394 https://doi.org/10.1104/pp.24.3.389
  14. Lauzer D, St-Arnaud M, Barabe D (1994) Tetrazolium staining and in vitro germination of mature seeds of Cypripedium acaule (Orchidaceae). Lindleyana 9:197-204
  15. Lee, JS, Choi BH (2006) Distribution and red data of wild orchids in the Korean Peninsula. Kor J Plant Taxon 36:335-360
  16. Lee TB (1984) Outline of Korean endemic plants and their distribution. Kor J Plant Taxon 14:21-32
  17. Lee WT (1996) Lineamenta Florae Koreae. Academy Press, Seoul
  18. Lee YN (2006) Flora of Korea. Gyohaksa, Seoul
  19. MacKay DB (1972) The measurement of viability. In: Roberts EH, ed. Viability of seeds. Syracuse, New York Syracuse University Press, pp 172-208
  20. Maekawa, F (1935) Diplolabellum coreanum. J Jpn Bot 11:305
  21. Malmgren S (1996) Orchid propagation: theory and practice. In: Allen, C. (Ed.), Proceedings of the North American Native Terrestrial Orchids: Propagation and Production. North American Native Terrestrial Orchid Conference, Maryland, pp 63-71
  22. Marasas WFO, Rheeder JP, Logrieco A, van Wyk PS, Juba JH (1998) Fusarium nelsonii and F. musarum: two new species in section Arthrosporiella related to F. camptoceras. Mycologia 90:505-513 https://doi.org/10.2307/3761409
  23. McLean KS, Ray KW (1988) Purple seed stain of soybean caused by isolates of Cercospora kikuchii from weeds. Can J Plant Path 10:166-171 https://doi.org/10.1080/07060668809501749
  24. Miyoshi K, Mii M (1998) Stimulatory effects of sodium and calcium hypochlorite, pre-chilling and cytokinins on the germination of Cypripedium macranthos seed in vitro. Physiol Plant 102:481-486 https://doi.org/10.1034/j.1399-3054.1998.1020401.x
  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  26. Paik WK (1999) The status of endemic plants in Korea and our tasks in the 21st century. Kor J Plant Taxon 29:263-273
  27. Rasmussen HN (1992) Seed dormancy patterns in Epipactis palustris (Orchidaceae): Requirements for germination and establishment of mycorrhiza. Physiologia Plantarum 86: 161-167 https://doi.org/10.1111/j.1399-3054.1992.tb01325.x
  28. SAS, Institute Inc. 2003. SAS version 9.0. SAS Institute, North Carolina
  29. Singh F (1981) Differential staining of orchid seeds for viability testing. American Orchid Soc Bull 50:416-418
  30. Stewart SL, Kane ME (2006a) Asymbiotic germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tiss Org Cult 86:147-158 https://doi.org/10.1007/s11240-006-9098-y
  31. Stewart SL, Kane ME (2006b) Symbiotic germination of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tiss Org Cult 86:159-167 https://doi.org/10.1007/s11240-006-9104-4
  32. Stewart SL, Kane ME (2007) Symbiotic seed germination and evidence for in vitro mycobionts specificity in Spiranthes brevilabris (Orchidaceae), and its implications for specieslevel conservation. In Vitro Cell Dev Biol Plant 43:178-186 https://doi.org/10.1007/s11627-006-9023-4
  33. St-Arnaud MD, Lauzer D, Barabe D (1992) In vitro germination and early growth of seedlings of Cypripedium acaule (Orchidaceae). Lindleyana 7:22-27
  34. Suzuki RM, Moreira VC, Nakabashi M, Ferreira WM (2009) Estudo da germinacao e crescimento in vitro de Hadrolaelia tenebrosa (Rolfe) Chiron & V.P. Castro (Orchidaceae), uma espécie da flora brasileira ameacada de extincao. Hoehnea 36:657-666 https://doi.org/10.1590/S2236-89062009000400006
  35. van Waes, JM, Debergh PC (1986) In vitro germination of some western European orchids. Physiol Plant 67:253-261 https://doi.org/10.1111/j.1399-3054.1986.tb02452.x
  36. Vujanovic V, St-Arnaud M, Barabe D, Thibeault G (2000) Viability testing of orchid seed and the promotion of colouration and germination. Ann Bot 86:79-86 https://doi.org/10.1006/anbo.2000.1162
  37. Wicklow DT, Bennet GA, Shotwell OL (1987) Secondary invasion of soybeans by Fusarium graminearum and resulting mycotoxin contamination. Plant Disease 71:1146
  38. Yildiz M, Celal ER (2002) The effect of sodium hypochlorite solution on in vitro seedling growth and shoot regeneration of flax (Linum usitatissimun). Natul wissen schffen 89: 259-261 https://doi.org/10.1007/s00114-002-0310-6

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