Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Fungicides on Phosphate Solubilization by Klebsiella oxytoca and Enterobacter ludwigii

  • Walpola, Buddhi Charana (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Keum, Mi-Jung (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2013.01.10
  • Accepted : 2013.02.25
  • Published : 2013.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of the present study was to isolate phosphate solubilizing bacteria (PSB) and to assess their potential tolerance to fungicides. Out of thirty PSB, two strains Klebsiella oxytoca and Enterobacter ludwigii were selected on the basis of their tolerance to fungicides. Both strains were assessed for their phosphate solubilizing ability using three different fungicides (difenoconazole, fluazinam and streptomycin) each with the concentrations of 0, 1, 2 or 3 times of the recommended rate. Both strains showed increased phosphate solubilization with difenoconazole at 1, 2 and 3 times of the recommended rate as compared to the phosphate solubilization of the control. The phosphate solubilization in Klebsiella oxytoca was recorded as 326, 538, 518 and 481 ${\mu}g\;mL^{-1}$ at 0, 1, 2 and 3 times of the recommended rate respectively, whereas in Enterobacter ludwigii it was recorded as 395, 499, 529 and 533 ${\mu}g\;mL^{-1}$ respectively at various doses. Based on the present findings, it may be concluded that both strains have the potential to be used as bio-inoculants which can solubilize phosphate even at the higher doses as compared to the recommended rate of fungicides.

Keywords

References

  1. Ahemad, M. and M.S. Khan. 2009. Effect of insecticide-tolerant and plant growth promoting Mesorhizobium on the performance of chickpea grown in insecticide stressed alluvial soils. J. Crop Sci. Biotechnol. 12:213-222.
  2. Ahemad, M. and M.S. Khan. 2011. Pseudomonas aeruginosa strain PS1enhances growth parameters of greengram [Vigna radiata (L.) Wilczek] in insecticide-stressed soils. J. Pest Sci. 84:123-131. https://doi.org/10.1007/s10340-010-0335-0
  3. Ahemad, M. and M.S. Khan. 2012a. Biotoxic impact of fungicides on plant growth promoting activities of phosphate solubilizing Klebsiella sp. Isolated from mustard (Brassica campestris) rhizosphere. J. Pest Sci. 85:29-36. https://doi.org/10.1007/s10340-011-0402-1
  4. Ahemad, M. and M.S. Khan. 2012b. Effect of fungicides on plant growth promoting activities of phosphate solubilizing Pseudomonas putida isolated from mustard (Brassica compestris) rhizosphere. Chemosphere. 86:945-950. https://doi.org/10.1016/j.chemosphere.2011.11.013
  5. Aktar, W., D. Sengupta, and A. Chowdhury. 2009. Impact of pesticides use in agriculture: their benefits and hazards. Interdiscip. Toxicol. 2:1-12. https://doi.org/10.2478/v10102-009-0001-7
  6. Bellinaso, M.L., C.W. Greer, M.C. Peralba, J.A. Henriques, and C.C. Gaylarde. 2003. Biodegradation of the herbicide trifluralin by bacteria isolated from soil. FEMS Microb. Ecol. 43:191-194. https://doi.org/10.1111/j.1574-6941.2003.tb01058.x
  7. Fox, J. E., J. Gulledge, E. Engelhaupt, M.E. Burow, and J.A. McLachlan. 2007. Pesticides reduce symbiotic efficiency of nitrogen-fixing rhizobia and host plants. Proc. Natl. Acad. Sci. USA. 104:10282-10287. https://doi.org/10.1073/pnas.0611710104
  8. Herman, P.L., M. Behrens, S. Chakraborty, B.M. Crastil, J. Barycki, and D.P. Weeks. 2005. A three component dicamba O-demethylase from Pseudomonas maltiphilia strain DI-6: gene isolation, characterization and heterologous expression. J. Biol. Chem. 280:24759-24767. https://doi.org/10.1074/jbc.M500597200
  9. Johnsen, K., C.S. Jacobsen, V. Torsvik, and J. Sorensen. 2001. Pesticide effects on bacterial diversity in agricultural soils-a review. Biol. Fert. Soils. 33:443-453. https://doi.org/10.1007/s003740100351
  10. Kumar, N., J.I. Anubhuti Bora, and M.K. Amb. 2010. Chronic toxicity of the triazole fungicide tebuconazole on a heterocystous, nitrogenfixing rice paddy field cyanobacterium, Westiellopsis prolific Janet. J. Microbiol. Biotechnol. 20: 1134-1139. https://doi.org/10.4014/jmb.0907.07013
  11. Kumar, S., K.G. Mukerji, and R. Lal. 1996. Molecular aspects of pesticide degradation by microorganisms. Crit. Rev. Microbiol. 22:1-26. https://doi.org/10.3109/10408419609106454
  12. MacLoughlin, T., J. Quinn, and A. Bettermann. 1992. Pseudomonas cepacia suppression of sunflower wilt fungus and role of antifungal compounds in controlling disease. Appl. Environ. Microbiol. 58:1760-1763.
  13. Murphy, J. and J.P. Riley. 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chem. Acta. 27:31-36. https://doi.org/10.1016/S0003-2670(00)88444-5
  14. Narison, V. and H.H. Patel. 1995. Studies on phosphate solubilizing microbes with special reference to fungi. PhD thesis. Bhavnagar University, Gujarat, India.
  15. Nautiyal, C. S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol. Lett. 170:265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
  16. Ortiz-Herna'ndez ML, Sa'nchez-Salinas E (2010) Biodegradation of the organophosphate pesticide tetrachlorvinphos by bacteria isolated from agricultural soils in Me'xico. Rev Int Contam Ambient 26:27-38.
  17. Oves, M., A. Zaidi, M.S. Khan, and M. Ahemad. 2009. Variation in plant growth promoting activities of phosphatesolubilizing microbes and factors affecting their colonization and solubilizing efficiency in different agro-ecosystems. In: Khan MS, Zaidi A (eds) Phosphate solubilizing microbes for crop improvement. Nova Science, New York, pp 247-263.
  18. Ramani, V. 2011. Effect of pesticides on phosphate solubilization by Bacillus sphaericus and Pseudomonas cepacia. Pestic. Biochem. Physiol. 99:232-236. https://doi.org/10.1016/j.pestbp.2011.01.001
  19. Sangodkar, U., P. Chapman, and A. Chakrabarty. 1998. Cloning, physical mapping and expression of chromosomal genes specifying degradation of the herbicide 2,4,5-T by Pseudomonas cepacia AC1100, Gene. 71:267-277.
  20. Sopid, S. 2012. Characterization of novel atrazine degrading Klebsiella sp. isolated from Thai agricultural soil. World Acad. Sci. Eng. Technol. 68: 1656-1658.
  21. Wani, P.A., A. Zaidi, A.A. Khan, and M.S. Khan. 2005. Effect of phorate on phosphate solubilization and indole acetic acid releasing potentials of rhizospheric microorganisms. Ann. Plant Prot. Sci. 13:139-144.
  22. Yasmin, H. and A. Bano. 2011. Isolation and characterization of phosphate solubilizing bacteria from rhizosphere soil of weeds of Khewra salt range and Atock. Pakistan J. Bot. 43: 1663-1668.
  23. Yu, X., X. Liu, T.H. Zhu, G.H. Liu, and C. Mao. 2011. Isolation and characterization of phosphate solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization. Biol. Fert. Soils. 47:437-446. https://doi.org/10.1007/s00374-011-0548-2