Bulletin of the Korean Chemical Society

  • 제34권6호
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  • Pages.1684-1688
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  • 2013
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Liquid Chromatography Quadrupole Time-Of-Flight Tandem Mass Spectrometry for Selective Determination of Usnic Acid and Application in Pharmacokinetic Study

  • Fang, Minfeng (Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University) ;
  • Wang, Hui (Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University) ;
  • Wu, Yang (Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University) ;
  • Wang, Qilin (Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University) ;
  • Zhao, Xinfeng (Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University) ;
  • Zheng, Xiaohui (Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University) ;
  • Wang, Shixiang (Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University) ;
  • Zhao, Guifang (Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University)
  • 투고 : 2013.01.28
  • 심사 : 2013.03.13
  • 발행 : 2013.06.20
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초록

A rapid and sensitive method for determining usnic acid of Lethariella cladonioides in rat was established using high performance liquid chromatography (HPLC) quadrupole time-of-flight (QTOF) tandem mass (MS/MS). Rat plasma was pretreated by mixture of acetonitrile and chloroform to precipitate plasma proteins. Chromatographic separation was achieved on a column ($50{\times}2.1$ mm, $5{\mu}m$) with a mobile phase consisting of water (containing $5{\times}10^{-3}$ M ammonium formate, pH was adjusted to 3.0 with formic acid) and acetonitrile (20:80, v/v) at a flow rate of 0.3 mL/min. A tandem mass spectrometric detection with an electrospray ionization (ESI) interface was conducted via collision induced dissociation (CID) under negative ionization mode. The MS/MS transitions monitored were m/z 343.0448 ${\rightarrow}$ m/z 313.2017 for usnic acid and m/z 153.1024 ${\rightarrow}$ m/z 136.2136 for protocatechuic acid (internal standard). The linear range was calculated to be 2.0-160.0 ng/mL with a detection limit of 3.0 pg/mL. The inter- and intra-day accuracy and precision were within ${\pm}7.0%$. Pharmacokinetic study showed that the apartment of usnic acid in vivo confirmed to be a two compartment open model. The method was fully valid and will probably be an alternative for pharmacokinetic study of usnic acid.

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참고문헌

  1. Ingolfsdottir, K. Phytochemistry 2002, 61, 729. https://doi.org/10.1016/S0031-9422(02)00383-7
  2. Bjerke, J. W.; Elvebakk, A.; Domínguez, E.; Dahlback, A. Phytochemistry 2005, 66, 337. https://doi.org/10.1016/j.phytochem.2004.12.007
  3. Huneck, S. Naturwissensch. 1999, 86, 559. https://doi.org/10.1007/s001140050676
  4. Molnar, K.; Farkas, E. Z. Naturforschung C 2010, 65, 157.
  5. Mitrovic, T.; Stamenkovic, S.; Cvetkovic, V.; Tosi , S.; Stankovic, M.; Radojevic, I.; Stefanovic, O.; Comi , L.; Da i , D.; Cur i , M.; Markovic, S. Int. J. Mol. Sci. 2011, 12, 5428. https://doi.org/10.3390/ijms12085428
  6. Kosani , M. M.; Rankovic , B. R.; Stanojkovic , T. P. J. Sci. Food. Agr. 2012, 92, 1909. https://doi.org/10.1002/jsfa.5559
  7. Guo, L.; Shi, Q.; Fang, J. L.; Mei, N.; Ali, A. A.; Lewis, S. M.; Leakey, J. E.; Frankos, V. H. J. Environ. Sci. Health. C 2008, 26, 317. https://doi.org/10.1080/10590500802533392
  8. Behera, B. C.; Mahadik, N.; Morey, M. Pharm. Biol. 2012, 50, 968. https://doi.org/10.3109/13880209.2012.654396
  9. O'Neill, M. A.; Mayer, M.; Murray, K. E.; Rolim-Santos, H. M.; Santos-Magalhaes, N. S.; Thompson, A. M.; Appleyard, V. C. Braz. J. Biol. 2010, 70, 659. https://doi.org/10.1590/S1519-69842010005000013
  10. Einarsdottir, E.; Groeneweg, J.; Bjornsdottir, G. G.; Harethardottir, G.; Omarsdottir, S.; Ingolfsdottir, K.; Ogmundsdottir, H. M. Planta. Medica. 2010, 76, 969. https://doi.org/10.1055/s-0029-1240851
  11. Song, Y. J.; Dai, F. J.; Zhai, D.; Dong, Y. M.; Zhang, J.; Lu, B. B.; Luo, J.; Liu, M. Y.; Yi, Z. F. Angiogenesis 2012, 15, 421. https://doi.org/10.1007/s10456-012-9270-4
  12. Bhattarai, H. D.; Paudel, B.; Hong, S. G.; Lee, H. K.; Yim, J. H. J. Nat. Med. 2008, 62, 481. https://doi.org/10.1007/s11418-008-0257-9
  13. Gupta, V. K.; Darokar, M. P.; Shanker, K.; Negi, A. S.; Srivastava, S. K.; Gupta, M. M.; Khanuja, S. P. S. J. Liq. Chromatogr. R. T. 2007, 30, 97. https://doi.org/10.1080/10826070601034345
  14. Cansaran, D.; Atakol, O.; Halici, M. G.; Aksoy, A. Pharm. Biol. 2007, 45, 77. https://doi.org/10.1080/13880200601028503
  15. Ji, X.; Khan, I. A. J. AOAC. Int. 2005, 88, 1265.
  16. Falk, A.; Green, T. K.; Barboza, P. J. Chromatogr. A 2008, 1182, 141. https://doi.org/10.1016/j.chroma.2007.12.035
  17. Dailey, R.; Siemion, R.; Raisbeck, M.; Jesse, C. J. AOAC. Int. 2010, 93, 1137.
  18. Roach, J. A. G.; Musser, S. M.; Morehouse, K.; Woo J. Y. J. J. Agr. Food. Chem. 2006, 54, 2484. https://doi.org/10.1021/jf052767m
  19. Wei, A. H.; Zhou D. N.; Ruan, J. L.; Cai, Y. L.; Xiong, C. M.; Li, M. X. J. Sci. Food. Agr. 2012, 92, 373. https://doi.org/10.1002/jsfa.4601

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