한국안광학회지 (Journal of Korean Ophthalmic Optics Society)

한국안광학회 (The Korean Ophthalmic Optics Society)
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각막 내 효소의 변성을 억제하는 안경 렌즈의 UV-B 차단율

The Inhibitory UV-B Blocking Rate of Eyeglasses Lens on the Enzymes Denaturation in Cornea

  • 김소라 (서울과학기술대학교 안경광학과) ;
  • 이지희 (서울과학기술대학교 안경광학과) ;
  • 최정임 (서울과학기술대학교 안경광학과) ;
  • 박미정 (서울과학기술대학교 안경광학과)
  • Kim, So Ra (Dep. of Optometry, Seoul National University of Science and Technology) ;
  • Lee, Jee Hee (Dep. of Optometry, Seoul National University of Science and Technology) ;
  • Choi, Jung-Im (Dep. of Optometry, Seoul National University of Science and Technology) ;
  • Park, Mijung (Dep. of Optometry, Seoul National University of Science and Technology)
  • 투고 : 2013.08.05
  • 심사 : 2013.09.14
  • 발행 : 2013.09.30
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초록

목적: 본 연구에서는 안구 내에 존재하는 효소들의 변성을 차단할 수 있는 안경렌즈의 UV-B 차단율을 밝히고자 하였다. 방법: RNase A와 catalase, superoxide dismutase(SOD)를 1, 3, 6, 24, 96시간 동안 312 nm의 UV-B에 노출시킨 후 아크릴아미드 겔 전기영동법으로 손상 정도를 확인하였다. 또한, 50%, 80%, 95%, 99%의 UV-B 차단율을 가진 안경렌즈의 효소 손상 억제 효과를 알아보았다. 결과: RNase A는 1시간 동안 UV-B의 노출에서 손상이 유발되었으며 1시간 이상 6시간 이하의 노출에 의한 효소의 손상을 막기 위해서는 95%, 24시간 이상 96시간 이하의 노출에는 99% UV-B 차단 렌즈가 효소의 손상을 완벽하게 억제하였다. Catalase는 UV-B에 대한 1시간 이하의 노출에는 영향을 받지 않아 아무런 변성이 발생하지 않았고, 3시간 이상 6시간 이하의 단시간 노출에서는 50% 이상의 UV-B 차단 렌즈, 24시간 이상 96시간 이하의 장시간 노출에는 95% 이상 UV-B 차단 렌즈로 효소의 변성을 완벽하게 억제할 수 있었다. SOD는 6시간 이하의 노출에는 손상되지 않았고, 24시간 정도의 노출에서도 50% 차단 렌즈로 효소 손상을 억제할 수 있었다. 그러나 96시간 UV-B에 노출될 경우는 95% 이상의 차단율을 가진 안경렌즈에 의해 SOD의 손상이 완벽하게 억제되었다. 결론: 본 연구에서는 UV-B 노출에 의한 효소의 변성을 억제하기에 적절한 안경렌즈 차단율은 각 효소마다 상이하였으며 일정 수준 이상의 UV-B 차단율을 가진 경우에만 효과가 있음을 밝혔다.

Purpose: To investigate the UV-B blocking rate of eyeglasses lens which can prevent enzymes denaturation in cornea. Methods: The denaturation degree of RNase A and catalase, superoxide dismutase (SOD) was determined by using Acrylamide gel electrophoresis after UV-B irradiation of 312 nm for 1, 3, 6, 24 and 96 hours. Also, the inhibitory effect of eyeglasses lens having UV-B blocking rate of 50%, 80%, 95% and 99% on the enzymes denatration was measured. Results: The denaturation of RNase A was induced by 1 hour-irradiation of UV-B. To inhibit RNase A denaturation after UV-B irradiation between 1 hour and 6 hours, UV-B blocking lens of 95% were effective. UV-B blocking lens of 99% suppressed the inhibition of RNase A denaturation after the UV-B exposure between 24 hours and 96 hours. The denaturation of catalase was not induced by 1 hourirradiation of UV-B. To inhibit enzyme denaturation after UV-B irradiation between 1 hour and 6 hours, UV-B blocking lens of 50% were effective. UV-B blocking lens of 95% suppressed the inhibition of enzyme denaturation induced by UV-B irradiation between 24 hours and 96 hours. The SOD denaturation was not induced by UV-B irradiation shorter than 6 hours exposure. The UV-B blocking lens of 50% could inhibit SOD denaturation after the UV-B irradiation for 24 hours. When SOD was exposed to UV-B for 96 hrs, SOD denaturation was inhibited by eyeglasses lens with UV blocking rate higher than 95%. Conclusions: The results demonstrated that the proper UV-B blocking rates of eyeglasses lens to inhibit the enzymes denaturatioin was different according to the types of enzymes and its inhibitory effect was effective only when eyeglasses lens had higher than certain UV-B blocking rate.

키워드

참고문헌

  1. Gallagher RP, Lee TK. Adverse effects of ultraviolet radiation : a brief review. Prog Biophys Mol Biol. 2006;92(1): 119-131. https://doi.org/10.1016/j.pbiomolbio.2006.02.011
  2. McCullough EC, Fullerton GD. Potential eye hazards of sunglasses. Surv Ophthalmol. 1971;16(1):108-111.
  3. Zigman S, Datiles M, Torczynski E. Sunlight and human cataracts. Invest Ophthal Vis Sci. 1979;18(5):462-467.
  4. Youn HY, McCanna DJ, Sivak JG, Jones LW. In vitro ultraviolet-induced damage in human corneal, lens, and retinal pigment epithelial cells. Mol Vis. 2011;17(1):237- 246.
  5. Elanchezhian R, Palsamy P, Madson CJ, Lynch DW, Shinohara T. Age-related cataracts: homocysteine coupled endoplasmic reticulum stress and suppression of Nrf2- dependent antioxidant protection. Chem Biol Interact. 2012;200(1):1-10. https://doi.org/10.1016/j.cbi.2012.08.017
  6. Fulgncio Cunha AA, Bosco AA, Veloso CA, Volpe CM, Chaves MM, Nogueira-Machado JA. Suppressive effect of aqueous humor from person with Type 2 diabetes with or without retinopathy on reactive oxygen species generation. Diabetes Res Clin Pract. 2013;100(1):69-73. https://doi.org/10.1016/j.diabres.2013.01.018
  7. Said T, Dutot M, Martin C, Beaudeux JL, Boucher C, Enee E, et al. Cytoprotective effect against UV-induced DNA damage and oxidative stress: role of new biological UV filter. Eur J Pharm Sci. 2007;30(3-4):203-210. https://doi.org/10.1016/j.ejps.2006.11.001
  8. American National Standards Institute (ANSI). American National Standard Requirements for Non-Prescription Sunglasses and Fashion Eyewear, Z80.3-1996, ANSI, New York, 1996.
  9. Yu DS. Yoo JS. Evaluation of ultraviolet blocking of ophthalmic lenses. J Korean Oph Opt Soc. 2008;13(3):7-12.
  10. Park YM, Park CS, Lee HS, Park MJ. The effect of UV blocking lens on the denaturation of RNase A induced by UV-A. J Korean Oph Opt Soc. 2007;12(1):9-15.
  11. Park CS, Park YM, Kim DH, Park MJ. The effect of UV blocking lens on the denaturation of antioxidative enzymes induced by UV-A. J Korean Oph Opt Soc. 2007;12(3):97- 103.
  12. Neuhoff V, Arold N, Taube D, Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis. 1988;9(6):255-262. https://doi.org/10.1002/elps.1150090603
  13. Bartlett EJ, Brissett NC, Doherty AJ. Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates. Proc Natl Acad Sci U S A. 2013;110(22):E1984-1991. https://doi.org/10.1073/pnas.1302616110
  14. Marchitti SA, Chen Y, Thompson DC, Vasiliou V. Ultraviolet radiation: cellular antioxidant response and the role of ocular aldehyde dehydrogenase enzymes. Eye Contact Lens. 2011;37(4):206-213. https://doi.org/10.1097/ICL.0b013e3182212642
  15. Babizhayev MA. Mitochondria induce oxidative stress, generation of reactive oxygen species and redox state unbalance of the eye lens leading to human cataract formation: disruption of redox lens organization by phospholipid hydroperoxides as a common basis for cataract disease. Cell Biochem Funct. 2011;29(3):183-206. https://doi.org/10.1002/cbf.1737
  16. Black AT, Gordon MK, Heck DE, Gallo MA, Laskin DL, Laskin JD. UVB light regulates expression of antioxidants and inflammatory mediators in human corneal epithelial cells. Biochem Pharmacol. 2011;81(7):873-880. https://doi.org/10.1016/j.bcp.2011.01.014
  17. Godenschwege T, Forde R, Davis CP, Paul A, Beckwith K, Duttaroy A. Mitochondrial superoxide radicals differentially affect muscle activity and neural function. Genetics. 2009;183(1):175-184. https://doi.org/10.1534/genetics.109.103515
  18. Formicki G, Stawarz R. Ultraviolet influence on catalase activity and mineral content in eyeballs of gibel carp (Carassius aurants gibelio). Sci Total Environ. 2006; 369(1-3):447-450. https://doi.org/10.1016/j.scitotenv.2006.07.021
  19. Reddy VN, Kasahara E, Hiraoka M, Lin LR, Ho YS. Effects of variation in superoxide dismutase(SOD) on oxidative stress and apoptosis in lens epithelium. Exp Eye Res. 2004;79(6):859-868. https://doi.org/10.1016/j.exer.2004.04.005
  20. Moon BY, Hwang KJ, Lee YJ, Yu DS. Changes of the plastic lens properties caused by etching of the coating films. J Korean Oph Opt Soc. 2010;15(1):55-60.

피인용 문헌

  1. The Relationship between Structural Denaturation of Antioxidative Enzymes and Their Enzyme Activity due to Repeated Exposure to UV-A vol.20, pp.1, 2015, https://doi.org/10.14479/jkoos.2015.20.1.75
  2. Denaturation and Inactivation of Antioxidative Enzymes due to Repeated Exposure to UV-B and Inhibitory Effect of RGP Lens vol.20, pp.2, 2015, https://doi.org/10.14479/jkoos.2015.20.2.237