The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Inhibitory Effect of Water Extract from Dojuksan on LPS-induced Proinflammatory Cytokines Production in RAW 264.7 Cells

LPS로 자극한 RAW 264.7 세포에서 염증성세포활성물질 생산에 미치는 도적산(導赤散) 물 추출의 억제 효과

  • Kim, Ji-Eun (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kim, Sung-Bae (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kang, Ok-Hwa (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Shin, In-Sik (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kang, Suk-Hoon (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Lee, Seung-Ho (Department of Rehabilitative Therapy in Oriental Medicine, Gimcheon University) ;
  • Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University)
  • 김지은 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 김성배 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 신인식 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 강석훈 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 이승호 (김천대학교 한방재활학과) ;
  • 권동렬 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소)
  • Received : 2013.04.22
  • Accepted : 2013.05.23
  • Published : 2013.05.30
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Abstract

Objectives : DojukSan is known to be effective for treating a urinary diseases and stomatitis. However, there has been a lack of studies regarding the effects of Dojuksan on the inflammatory activities and effector inflammatory disease mechanism about macrophage before is not known. To elucidate the molecular mechanisms of Dojuksan water extract (DJS) on pharmacological and biochemical actions in inflammation, we examined the effect of DJS on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated macrophages. Methods : In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and western blot analysis to measure the activation of MAPKs. Cells were treated with 200 ng/mL of LPS 1 h prior to the addition of DJS. Cell viability was measured by MTS assay. The investigation focused on whether DJS inhibited nitric oxide (NO) and prostaglandin E2 ($PGE_2$) productions, as well as the expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated RAW 264.7 cells. Results : We found that DJS inhibited LPS-induced NO, $PGE_2$ and IL-6 productions as well as the expressions of iNOS and COX-2. Furthermore, DJS suppressed the LPS-induced phosphorylation of p38 MAPK and c-Jun NH2-protein kinase (JNK). Conclusions : These results suggest that DJS has inhibitory effects on LPS-induced $PGE_2$, NO, and IL-6 production, as well as the expressions of iNOS and COX-2 in the murine macrophage. These inhibitory effects occur through blockades on the MAPKs phosphorylation.

Keywords

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