생명과학회지 (Journal of Life Science)

  • 제23권5호
  • /
  • Pages.698-702
  • /
  • 2013
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

GABA 고함유 흑찰거대배아미의 투여가 C57BL/6형 생쥐의 알코올 섭취량에 미치는 영향

Effect of Feeding with High γ-Aminobutyric Acid (GABA) Containing Giant Embryo Black Sticky Rice (Oryza sativa L.) on Alcohol Intake in C57BL/6 Mice

  • 김현경 (부산대학교 의학연구원) ;
  • 김성곤 (양산부산대학교병원 정신건강의학과) ;
  • 이진성 (양산부산대학교병원 정신건강의학과) ;
  • 이상신 (고신대학교복음병원 정신건강의학과) ;
  • 정우영 (양산부산대학교병원 정신건강의학과) ;
  • 한상익 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 김병주 (농촌진흥청 국립식량과학원 기능성작물부)
  • Kim, Hyeun-Kyeung (Medical Research Institute, Pusan National University) ;
  • Kim, Sung-Gon (Department of Psychiatry, Pusan National University Yangsan Hospital) ;
  • Lee, Jin-Sung (Department of Psychiatry, Pusan National University Yangsan Hospital) ;
  • Lee, Sang-Shin (Department of Psychiatry, Kosin University Gospel Hospital) ;
  • Jung, Woo-Young (Department of Psychiatry, Pusan National University Yangsan Hospital) ;
  • Han, Sang-Ik (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Kim, Byung-Ju (Department of Functional Crop, National Institute of Crop Science, RDA)
  • 투고 : 2013.02.22
  • 심사 : 2013.05.23
  • 발행 : 2013.05.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 실험은 일반미보다 GABA 함량이 많은 흑찰거대배아미(밀양263호) 및 거대배아미와 일반미를 C57BL/6 생쥐에게 16일 동안 자유 섭취시키면서 2시간 알코올 섭취량, 22시간 물 섭취량 및 체중의 변화 등을 조사하였다. 그 결과 흑찰거대배아미 및 거대배아미와 일반미 3군과 2시간 알코올 섭취량의 일변화에 대해 repeated measure ANOVA를 실시하였을 때, 유의한 차이가 관찰되었다(df=16, F=3.109, p=0.004). 일별 2시간 알코올 섭취량에 대하여 흑찰거대배아미군과 일반미군의 양군간 비교시 일반미군에 비하여 흑찰거대배아미군에서 자유 섭취 후 2일째부터 16일째까지 지속적으로 유의하게 감소하였다. 또한 흑찰거대배아미군과 일반미군 2군을 각각 군내 비교를 하였을 때, 일반미 섭취군의 경우에는 섭취전과 유의한 차이가 없었으나, 흑찰거대배아미 군에서는 섭취 전에 비하여 섭취 후 4일째부터 16일째까지 유의하게 감소하였다. 한편 22시간 물 섭취량의 일변화와 체중의 일변화에 대해서 repeated measure ANOVA를 실시하였을 때에도 유의한 교차가 없었다. 그러나 쌀 섭취량의 일변화에 대한 repeated measure ANOVA를 실시하였을 때에 유의한 차이가 관찰되었다(df=16, F=7.145, p=0.001). 이러한 2시간 알코올 섭취량의 변화의 결과는 일반미와의 성분비교에서 흑찰거대배아미에 많이 함유되어 있는 GABA 등에 의한 것으로 추정할 수 있다.

It is known that black sticky rice with giant embryo (BSRGB, Milyang 263) and giant embryo rice (GER) contains higher levels of GABA than rice. Therefore, feeding BSRGB, GER, or rice freely to C57BL/6 mice with two-hour alcohol intake for 16 days was investigated. For two-hour alcohol intake, a repeated measure ANOVA (three treatment groups repeated across 16 days; 8 two-day blocks) yielded a significant group by block interaction (df=16, F=3.109, p=0.004). The independent t-test showed that significant suppression of two-hour alcohol intake was observed when subjects were administered with BSRGB, compared with the rice alone across all the two-day blocks (p<0.05). The paired t-test revealed that a significant suppression of two-hour alcohol intake was observed starting 4 to 16 days after freely feeding with BSRGB compared to before feeding. However, there is no significant difference in the two-hour alcohol intake observed between the before and after administration of rice. A repeated measure ANOVA revealed no significant group by block interaction for 22-hour water intake and body weight. However, a repeated measure ANOVA revealed a significant grouping by block interaction for food intake. These results indicate a change of two-hour alcohol intake is presumably caused by GABA, which is found in higher levels in BSRGB than in rice.

키워드

참고문헌

  1. Chang, Y. T., Sun, H. S., Fann, C. S., Chang, C. J., Liao Z. H., Huang, J. L., Loh, W. H., Yu, W. Y. and Chenget, A. T. A. 2002. Association of the gamma-aminobutyric acid A receptor gene cluster with alcohol dependence in Taiwanese Han. Mol Psychiatry 7, 828-829. https://doi.org/10.1038/sj.mp.4001110
  2. Dahchour, A. and De Witte, P. 2000. Ethanol and amino acids in the central nervous system: assessment of the pharmacological actions of acamprosate. Prog Neurobiol 60, 343-362. https://doi.org/10.1016/S0301-0082(99)00031-3
  3. Jeon, G. U., Lee, M. Y., Yoon, J. M., Jang, S. H., Jung, M. R., Jeong, H. S. and Lee, J. S. 2010. Effects of heat treatment and selected medicinal plant extracts on GABA content after germination. J Korean Soc Food Sci Nutr 39, 154-158. https://doi.org/10.3746/jkfn.2010.39.1.154
  4. Jung, I. S., Kim, Y. J., Choi, I. S., Choi, E. Y., Shin, S. H., Gal, S. W. and Choi, Y. J. 2007. Studies on antioxidant activity and inhibition of nitric oxide synthesis of germinated brown rice soaked in mycelial culture broth of Phellinus linteus. J Life Sci 17, 1141-1146. https://doi.org/10.5352/JLS.2007.17.8.1141
  5. Kim, S. G., Han, B. D., Park, J. M. and Kim, M. J. 2001. Effect of combined administration of acamprosate and naltrexone on alcohol intake C57BL/6 mice. J Korean Neuropsychiatr Assoc 40, 981-990
  6. Kim, S. G., Han, B. D., Park, J. M., Kim, M. J. and Stromberg, M. F. 2004. Effects of the combination of naltrexone and acamprosate on alcohol intake in mice. Psychiatry Clinic Neurosci 58, 30-36. https://doi.org/10.1111/j.1440-1819.2004.01189.x
  7. Kim, H. J., Kim, H., Jun, B., Cha, J. Y., Kim, H, K. and Cho, Y. S. 2001. Analysis of $\gamma$-aminobutyric acid concentrations in Korean plants and mushrooms. J Life Sci 11, 537-542.
  8. Lappalainen, J., Krupitsky, E., Remizov, M., Pchelina, S., Taraskina, A., Zvartau, E., Somberg, L. K., Covault, J., Kranzler, H. R., Krystal, J. H. and Gelernter, J. 2005. Association between alcoholism and $\gamma$-amino butyric acid $\alpha$2 receptor subtype in a Russian population. Alcohol Clin Exp Res 29, 493-498. https://doi.org/10.1097/01.ALC.0000158938.97464.90
  9. Lee, S. M., Chung, W. J., Kim, I. S., Kim, H. J., Cho, W. H., Shin, E. C., Ahn, S. H., Han, K. H. and Myoung, J. I. 2008. Socioeconomic costs of alcohol drinking in Korea. J Korean Acad Fam Med 29, 201-212.
  10. Loh, E. W., Smith, I., Murray, R., McLaughlin, M., McNulty, S and Ball, D. 1999. Association between variants at the $GABA_{A}{\beta}2$, $GABA_{A}{\alpha}6$ and $GABA_{A}{\gamma}2$ gene cluster and alcohol dependence in a Scottish population. Mol Psychiatry 4, 539-544. https://doi.org/10.1038/sj.mp.4000554
  11. Oh, S. H., Kim, S. H., Moon, Y. J. and Choi, W. G. 2002. Changes in the leveles of $\gamma$ aminobutyric acid and some amino acid by application of a glutamic acid solution for the germination of brown rices. Korean J Biotechnol Bioeng 17, 49-53.
  12. Ramputh, A. I. and Bown, A. W. 1996. Rapid $\gamma$-aminobutyric acid synthesis and the inhibitation of the growth and development of oblique-banded leaf-roller larvae. Plant Physiol 111, 1349-1352.
  13. Social Statistics Survey. 2006. National Statistics Office. Korea
  14. Song, J., Koller, D. L., Foroud, T., Carr, K., Zhao, J., Rice, J., Nurnberger Jr, J. I., Begleiter, H., Porjesz, B., Smith, T. L., Schuckit, M. A. and Edenberg, H. J. 2003. Association of GABAA receptors and alcohol dependence and the effects of genetic imprinting. Am J Med Genet B Neuropsychiatr Genet 117B, 39-45. https://doi.org/10.1002/ajmg.b.10022
  15. Wegelius, C. J., Roberts, A. J., Schulteis G. and Koob, G. F. 1999. Central administration of an opiate antagonist decreases oral ethanol self- administration in rats. Alcohol Clin Exp Res 23, 1468-1476. https://doi.org/10.1111/j.1530-0277.1999.tb04669.x

피인용 문헌

  1. Effect of GABA Extract of Black Sticky Rice with Giant Embryo on Alcohol-Related Indices After Acute Alcohol Intake in Social Drinkers vol.39, pp.7, 2015, https://doi.org/10.1111/acer.12746
  2. Effect of Feeding High Gamma-Aminobutyric Acid-Containing Giant Embryo Black Sticky Rice (Oryza sativa L.) on Anxiety-Related Behavior of C57BL/6 Mice vol.20, pp.8, 2017, https://doi.org/10.1089/jmf.2017.3928