생명과학회지 (Journal of Life Science)

  • 제23권4호
  • /
  • Pages.510-517
  • /
  • 2013
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

초임계 추출 계피오일의 3T3-L1 지방전구세포의 분화 전사인자 억제에 의한 지방대사 조절

Inhibition of Adipocyte Differentiation and Adipogenesis by Supercritical Fluid Extracts and Marc from Cinnamomum verum

  • 박성진 (계명대학교 식품가공학 전공) ;
  • 이삼빈 (계명대학교 식품가공학 전공) ;
  • 이인선 (계명대학교 식품가공학 전공) ;
  • 유미희 (계명대학교 식품가공학 전공)
  • Park, Sung-Jin (Department of Food Science and Technology, Keimyung University) ;
  • Lee, In-Seon (Department of Food Science and Technology, Keimyung University) ;
  • Lee, Sam-Pin (Department of Food Science and Technology, Keimyung University) ;
  • Yu, Mi-Hee (Department of Food Science and Technology, Keimyung University)
  • 투고 : 2013.01.14
  • 심사 : 2013.04.03
  • 발행 : 2013.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 초임계를 이용한 계피 오일 추출물(SFC)과 오일 추출 후 남은 부산물인 박(SFM), 그리고 80% methanol (ME) 계피추출물을 이용하여 항비만 효과를 비교하고 어떤 계피의 어떤 성질의 성분이 비만에 더 효과적인지 알아보았다. 3T3-L1 preadipocyte의 성숙한 지방세포로 분화시키기 위해 iso-butylmethylanthine (IBMX), dexamathasone, insulin을 SFC, SFM, ME를 처리하고 Real time PCR을 이용하여 전사인자 발현을 확인하였다. 그 결과 SFC에서 mRNA 수준에서 peroxisome-proliferators-activated-receptor-${\gamma}$ ($PPAR{\gamma}$), CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$)의 저해능이 세 가지 조건 중에서 가장 높았으며, 또한 SFC는 peroxisome-proliferators-activated-receptor-${\gamma}$ ($PPAR{\gamma}$), CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$) sterol-regulatory-element-binding protein-1c (SREBP1c)와 acyl-CoA synthetase-1 (ASC1), fatty acid synthesis (FAS), fatty acid transport-1 (FATP1), fatty acid binding protein-4 (FABP4) 그리고 perilipin의 전사인자도 농도유의적으로 감소시켰다.

This study was performed to evaluate the antiobesity effect of supercritical fluid extracts (SFC) and marc methanol extracts (SFM) from Cinnamomum verum in 3T3-L1 preadipocytes. In inducing the differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, iso-butylmethylanthine (IBMX), dexamathasone, and insulin, treatment with fraction residue SFC and SFM. SFC significantly reduced the mRNA expression of the transcription factor peroxisome proliferator-activate-dreceptor-${\gamma}$ ($PPAR{\gamma}$), the sterol regulatory-element-binding protein-1c (SREBP1c), and the CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$) in a concentration-dependent manner. Moreover, SFC markedly down-regulated acyl-CoA synthetase-1 (ASC1), fatty acid synthesis (FAS), fatty acid transport-1 (FATP1), fatty acid binding protein 4 (FABP4), and perilipin. These findings suggest that SFC may be a potential therapeutic adjunct for obesity by targeting the differentiation of preadipocytes, as well as their functions.

키워드

참고문헌

  1. Albu, J., Allison, D., Boozer, C. N., Heymsfield, S., Kissileff, H., Kretser, A., Krumhar, K., Leibei, R., Nonas, C., Pi-Sunyer, X., Vanltallie, T. and Wedral, E. 1997. Obesity solutions: report of a meeting. Nutr Rev 55, 150-156.
  2. Apfelbaum, M., Vague, P., Ziegler, O., IIanotin, O., Thomas, F. and Leu-tenegger, E. 1999. Long-term maintenance of weight loss after a very low calorie diet: Efficacy and tolerability of sibutramine. Am J Med 106, 179-184. https://doi.org/10.1016/S0002-9343(98)00411-2
  3. Barth, M. M., Zhou, C., Kute, M. K. and Rosenthal, G. A. 1995. Determination of optimum conditions for supercritical fluid extraction of carotenoids (Daucus carota L.) from carrot tissue. J Agric Food Chem 43, 2876-2878. https://doi.org/10.1021/jf00059a019
  4. Carmen, S. 2000. Processing technologies: An alternativefor cactus pear (Opuntia spp) fruits and cladodes. J Aarid Environ 46, 209-225. https://doi.org/10.1006/jare.2000.0676
  5. Cole, S. P. 1986. Rapid chemosensitivity testing of human lung tumor cells using the MTT assay. Cancer Chemother Pharmacol 17, 259-263.
  6. Farmer, S. R. 2006. Transcriptional control of adipocyte formation. Cell Metab 4, 263-273. https://doi.org/10.1016/j.cmet.2006.07.001
  7. Green, H. and Kehinde, O. 1974. Sublines of mouse 3T3 cells that accumlate lipid. Cell 1, 113-116. https://doi.org/10.1016/0092-8674(74)90126-3
  8. Gregoire, F. M., Smas, C. M. and Sul, H. S. 1998. Understanding adipocyte differentiation. Physiol Rev 78, 783-809.
  9. Grundy, S. M. 1998. Multifactorial causation of obesity: implications for prevention. Am J Clin Nutr 67, 563-572.
  10. Hansen, D. L., Toubro, S., Stock, M. J., Macdonald, I. A. and Astrup, A. 1998. Thermogenic effects of sibutramine in humans. Am J Clin Nutr 68, 1180-1186.
  11. Huang, B., Yuan, H. D., Kim, D. Y., Quan, H. Y. and Chung, S. H. 2011. Cinnamaldehyde prevents adipocyte differentiation and adipogenesis via regulation of peroxisome proliferator-activated receptor-${\gamma}$ (PPAR${\gamma}$) and AMP-activated protein kinase (AMPK)Pathways. J Agric Food Chem 59, 3666-3673. https://doi.org/10.1021/jf104814t
  12. Kannappan, S., Jayaraman, T., Rajasekar, P., Ravichandran, M. K. and Anuradha, C. V. 2006. Cinnamon bark extract improves glucose metabolism and lipid profile in the fructose- fed rat. Singapore Med J 47, 858-863.
  13. Kaul, P. N., Bhattacharya, A. K., Rao, B. R., Syamasundar, K. V. and Ramesh, S. 1987. Volatile constituents of essential oils isolated from different parts of cinnamon (Cinnamomum zeylanicum Blume). J Sci Food Agric 83, 53-55.
  14. Khan, A., Bryden, N. A., Polansky, M. M. and Anderson, R. A. 1990. Insulin potentiating factor and chomium content of selected foods and spices. Biol Trace Elem Res 24, 183-188. https://doi.org/10.1007/BF02917206
  15. Kim, N. M., Sung, H. S. and Woo, W. J. 1993. Effect of sol-vents and some extraction condition on antioxidant activity in Cinnamon extracts. Korean J Food Sci Technol 25, 204-209.
  16. Kim, K. I., Han, C. K., Seong, K. S., Lee, O. H., Park, J. M. and Lee, B. Y. 2003. Effect of whole powder and extracts of Gastrodiae Rhizomaon serum lipids and body fat in rats fed high-fat diet. Korean J Food Sci Technol 35, 720-725.
  17. Kim, W. J., Cho, J. Y., Choi, C. S., Yoon, G. S., Lee, W. K. and Ryu, Y. W. 2008. Antimicrobial Activity of Extracted by Supercritical Fluid from Origanum vulgare, Cinnamomum cassia, Chamaecyparis obtusa and Scutellariae baicalensis. Korean J Biotechnol Bioeng 22, 147-152.
  18. Kim, S. J., Lee, M. K., Back, S. S. and Chun, B. S. 2007. Extraction and identification of volatile isothiocyanates from Wasabi using supercritical carbon dioxide. Korean J Biotechnol Bioeng 22, 174-178.
  19. Lee, K. S., Kim, M. G. and Lee, N. Y. 2004. Antimicrobial effect of the extracts of Cactus Cheonnyuncho (Opuntiahumifusa) against food borne pathogens. J Korean Soc Food Nutr 33, 1268-1272. https://doi.org/10.3746/jkfn.2004.33.8.1268
  20. Lee, Y. A. and Kim, M. H. 2008. The effect of sea tangle extract on serum lipid level in ovariectomized rats. J Life Sci 18, 249-254. https://doi.org/10.5352/JLS.2008.18.2.249
  21. Lew, E. A. 1985. Mortality and weight: Insured lives and the american cancer society studies. Ann Intern Med 103, 1024-1029. https://doi.org/10.7326/0003-4819-103-6-1024
  22. MacDougald, O. A. and Lane, M. D. 1995. Transcriptional regulation of gene expressionduring adipocyte differentiation. Annu Rev Biochem 64, 345-373. https://doi.org/10.1146/annurev.bi.64.070195.002021
  23. Otto, T. C. and Lane, M. D. 2005. Adipose development: from stem cell to adipocyte. Crit Rev Biochem Mol Biol 40, 229-242. https://doi.org/10.1080/10409230591008189
  24. Park, M. K., Lee, Y. L. and Kang, E. S. 2005. Hepatoprotective effect of chounnyouncho extract in rats treated carbon tetrachloride. Korean J Food Sci Technol 37, 822-826.
  25. Park, S. M., Ahn, I. S., Hong, S. M., Kim, D. S., Kwon, D. Y. and Yang, H. J. 2010. The effects of the supplementation of Opuntia humifusa water extracts and methyl sulfonyl methane on the laying productivity, egg quality and sensory characteristics. J Korean Soc Food Sci Nutr 39, 294-300. https://doi.org/10.3746/jkfn.2010.39.2.294
  26. Park, S. J., Yu, M. H., Kim, J. E., Lee, S. P. and Lee, I. S. 2012. Comparison of antioxidant and antimicrobial activities of supercritical fluid extracts and marc extracts from Cinnamomum verum. J Life Sci 22, 373-379. https://doi.org/10.5352/JLS.2012.22.3.373
  27. Peter, G. K. 2000. Obesity as a medical problem. Nature 404, 635-643.
  28. Plaisier, C., Cok, A., Scott, J., Opejin, A., Bushhouse, K. T., Salie, M. J. and Louters, L. L. 2011. Effects of cinnamaldehyde on the glucose transport activity of GLUT1. Biochimie 93, 339-344. https://doi.org/10.1016/j.biochi.2010.10.006
  29. Rolls, B. J., Shide, D. J., Thorwart, M. L., and Ulbrecht, J. S. 1998. Sibutraminereduces food intake in nondieting women with obesity. Obes Res 6, 1-11. https://doi.org/10.1002/j.1550-8528.1998.tb00308.x
  30. Rosen, E. D. and Spiegelman, B. M. 2000. Molecular regulation of adipogenesis. Annu Rev Cell Dev Biol 16, 145-171. https://doi.org/10.1146/annurev.cellbio.16.1.145
  31. Rosen, E. D., Walkey, C. J., Puigserver, P. and Spiegelman, B. M. 2000. Transcriptional regulation of adipogenesis. Genes Dev 14, 1293-1307.
  32. Scott, M. G. 2004. Obesity, metabolic syndrome, and cardiovascular disease. J Clin Endocrinol Metab 89, 2595-2600. https://doi.org/10.1210/jc.2004-0372
  33. Shim, J. H., Kim, M. R. and Kim, M. R. 2001. A Comparative Study on the Solvent Extraction and Supercritical Fluid Extration Method of ${\beta}$-Ecdysone in Achyranthis radix. J Korean Soc Appl Chem Biolthechnol 44, 197-201.
  34. Spiegelman, B. M. 2001. Obesity and the regulation of energy balance. Cell 104, 531-543. https://doi.org/10.1016/S0092-8674(01)00240-9
  35. Spiegelman, B. M. and Flier, J. S. 1996. Adipogenesis and obesity: rounding out the big picture. Cell 87, 377-389. https://doi.org/10.1016/S0092-8674(00)81359-8
  36. Sreel, R. G. D. and Torrie, J. H. 1990. Principles and procedures of satistics. McGraw Hill, New York, USA.
  37. Wellman, N. S. 2002. Causes and consequences of adult obesity:health, social and economic impacts in the united States. Asia Pac J Clin Nutr 11, S705-S709. https://doi.org/10.1046/j.1440-6047.11.s8.6.x
  38. Wyatt, S. B., Winters, K. P. and Dubbert, P. M. 2006. Overweight and obesity: prevalence, consequences, and causes of a growing public health problem. Am J Med Sci 331, 166-174. https://doi.org/10.1097/00000441-200604000-00002
  39. Yoon, J. A. and Son, Y. S. 2009. Effects of Opuntia ficus-indica complexes B (OCB) on blood glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J Food Nutr 22, 48-56.
  40. Wijesekera, R. O. B., Jayewardene, A. L. and Rajapakse, L. S. 1974. Volatile constituents of leaf, stem and root oils of cinnamon (Cinnamomum zeylanicum). J Sci Food Agric 25, 1211-1220. https://doi.org/10.1002/jsfa.2740251004
  41. Zhang, J. W., Klemm, D. J., Vinson, C. and Lane, M. D. 2000. Role of CREB in transcriptional regulation of CCAAT/enhancer-binding protein beta gene during adipogenesis. J Biol Chem 279, 4471-4478.

피인용 문헌

  1. Co-treatment with Fermented Black Raspberry and Red Ginseng Extracts Improves Lipid Metabolism and Obesity in Rats Fed with a High-fat and High-cholesterol Diet vol.47, pp.3, 2015, https://doi.org/10.9721/KJFST.2015.47.3.364
  2. Study of Lipoprotein Lipase Inhibitory Activity of Anti-obesity Herb Extracts vol.47, pp.2, 2015, https://doi.org/10.9721/KJFST.2015.47.2.246
  3. Anti-adipogenic Effect of Fermented Coffee with Monascus ruber Mycelium by Solid-State Culture of Green Coffee Beans vol.43, pp.4, 2014, https://doi.org/10.3746/jkfn.2014.43.4.624
  4. Physiological Activities and Inhibitory Effect of Extracts of Cynanchi wilfordii Radix and Perilla sikokiana against Cell Differentiation in 3T3-L1 Adipocytes vol.45, pp.5, 2016, https://doi.org/10.3746/jkfn.2016.45.5.642
  5. Inhibitory Effect of Water Extracts of Aconiti Lateralis Preparata Radix and Acanthopanacis Cortex on Differentiation of Bone Marrow-Derived Adipocytes and Osteoclasts vol.22, pp.1, 2014, https://doi.org/10.14374/HFS.2014.22.1.151
  6. Influence of Galgeun-tang on Gene Expression in Diet-Induced Obese Mice vol.26, pp.2, 2016, https://doi.org/10.18325/jkmr.2016.26.2.1