Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibition of Adipocyte Differentiation and Adipogenesis by Supercritical Fluid Extracts and Marc from Cinnamomum verum

초임계 추출 계피오일의 3T3-L1 지방전구세포의 분화 전사인자 억제에 의한 지방대사 조절

  • Park, Sung-Jin (Department of Food Science and Technology, Keimyung University) ;
  • Lee, In-Seon (Department of Food Science and Technology, Keimyung University) ;
  • Lee, Sam-Pin (Department of Food Science and Technology, Keimyung University) ;
  • Yu, Mi-Hee (Department of Food Science and Technology, Keimyung University)
  • 박성진 (계명대학교 식품가공학 전공) ;
  • 이삼빈 (계명대학교 식품가공학 전공) ;
  • 이인선 (계명대학교 식품가공학 전공) ;
  • 유미희 (계명대학교 식품가공학 전공)
  • Received : 2013.01.14
  • Accepted : 2013.04.03
  • Published : 2013.04.30
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Abstract

This study was performed to evaluate the antiobesity effect of supercritical fluid extracts (SFC) and marc methanol extracts (SFM) from Cinnamomum verum in 3T3-L1 preadipocytes. In inducing the differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, iso-butylmethylanthine (IBMX), dexamathasone, and insulin, treatment with fraction residue SFC and SFM. SFC significantly reduced the mRNA expression of the transcription factor peroxisome proliferator-activate-dreceptor-${\gamma}$ ($PPAR{\gamma}$), the sterol regulatory-element-binding protein-1c (SREBP1c), and the CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$) in a concentration-dependent manner. Moreover, SFC markedly down-regulated acyl-CoA synthetase-1 (ASC1), fatty acid synthesis (FAS), fatty acid transport-1 (FATP1), fatty acid binding protein 4 (FABP4), and perilipin. These findings suggest that SFC may be a potential therapeutic adjunct for obesity by targeting the differentiation of preadipocytes, as well as their functions.

본 연구에서는 초임계를 이용한 계피 오일 추출물(SFC)과 오일 추출 후 남은 부산물인 박(SFM), 그리고 80% methanol (ME) 계피추출물을 이용하여 항비만 효과를 비교하고 어떤 계피의 어떤 성질의 성분이 비만에 더 효과적인지 알아보았다. 3T3-L1 preadipocyte의 성숙한 지방세포로 분화시키기 위해 iso-butylmethylanthine (IBMX), dexamathasone, insulin을 SFC, SFM, ME를 처리하고 Real time PCR을 이용하여 전사인자 발현을 확인하였다. 그 결과 SFC에서 mRNA 수준에서 peroxisome-proliferators-activated-receptor-${\gamma}$ ($PPAR{\gamma}$), CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$)의 저해능이 세 가지 조건 중에서 가장 높았으며, 또한 SFC는 peroxisome-proliferators-activated-receptor-${\gamma}$ ($PPAR{\gamma}$), CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$) sterol-regulatory-element-binding protein-1c (SREBP1c)와 acyl-CoA synthetase-1 (ASC1), fatty acid synthesis (FAS), fatty acid transport-1 (FATP1), fatty acid binding protein-4 (FABP4) 그리고 perilipin의 전사인자도 농도유의적으로 감소시켰다.

Keywords

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