생명과학회지 (Journal of Life Science)

  • 제23권4호
  • /
  • Pages.495-500
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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무당벌레에서 유래된 HaGF peptide를 이용한 염증 억제 효과

Anti-inflammatory Effect of HaGF peptide of Harmonia axyridis

  • 김동희 (한국한방산업진흥원) ;
  • 김현정 (대구한의대학교 화장품약리학과) ;
  • 이진영 (호서대학교 한방화장품과학과) ;
  • 황재삼 (국립농업과학원 농업생물부) ;
  • 김인우 (국립농업과학원 농업생물부) ;
  • 이슬기 (대구한의대학교 화장품약리학과) ;
  • 정현국 (대구한의대학교 화장품약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Kim, Dong-Hee (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Hyeon-Jeong (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Hwang, Jae-Sam (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Kim, In-Woo (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lee, Seul-Gi (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Jeong, Hyeon-Guk (Department of Cosmeceutical Science, Daegu Haany University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
  • 투고 : 2012.11.12
  • 심사 : 2013.04.22
  • 발행 : 2013.04.30
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초록

본 연구에서는 무당벌레 유충으로부터 분리된 항균 펩타이드 유전자의 일부 영역인 HaGF를 이용하여 대식세포의 염증에 미치는 영향을 조사하였다. 세포는 LPS 처리 후 한 시간 뒤에 HaGF를 처리를 하여, 세포 독성이 나타나지 않는 농도인 5, 25, 50, 100 ${\mu}g/ml$을 사용하였다. 그 결과 HaGF가 염증성 cytokine의 생성을 감소시키는 것을 확인 하였으며 iNOS와 COX-2 역시 100 ${\mu}g$/ml의 농도에서 각각 51%, 49% 저해율을 보였다. 따라서, HaGF는 LPS로 유도된 대식세포주인 Raw 264.7 세포에서의 염증 반응 억제 효과를 기대할 수 있었다.

Harmonia axyridis is known to display diverse biological activities, such as growth promotion. However, few studies have investigated the effect of H. axyridis on inflammation of the skin. In this study, we explored the anti-inflammatory effect of the Harmoniasin gene fragment (HaGF) peptide from H. axyridis on macrophage cells. During the entire experimental period, 5, 25, 50, and 100 ${\mu}g/ml$ of HaGF showed no cytotoxicity. However, at these concentrations, HaGF inhibited the activity of iNOS and COX-2 by 51% and 49%, respectively. In addition, the HaGF extract reduced the release of inflammatory cytokines, including TNF-a and IL-6. Therefore, HaGF has been LPS-induced macrophage Raw 264.7 cells could be expected from the inhibitory effects of the inflammatory.

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피인용 문헌

  1. in RAW 264.7 cells vol.51, pp.4, 2018, https://doi.org/10.4163/jnh.2018.51.4.323