생명과학회지 (Journal of Life Science)

  • 제23권4호
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  • Pages.479-486
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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대장균 세포 내 다양한 외부 스트레스에 대한 DPS 단백질의 생리적 기능

Physiological Function of a DNA-Binding Protein from Starved Cells in Combating Diverse External Stresses in Escherichia coli

  • 이주형 (경상대학교 사범대학 부설고등학교) ;
  • 정수진 (경상대학교 사범대학 부설고등학교) ;
  • 오훈택 (경상대학교 응용생명과학부) ;
  • 김외연 (경상대학교 응용생명과학부) ;
  • 정영준 (경상대학교 응용생명과학부)
  • Lee, Joo Hyeong (Senior High School of Gyeongsang National University) ;
  • Cheong, Su Jin (Senior High School of Gyeongsang National University) ;
  • Oh, Hun Taek (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Woe Yeon (Division of Applied Life Science, Gyeongsang National University) ;
  • Jung, Young Jun (Division of Applied Life Science, Gyeongsang National University)
  • 투고 : 2013.02.01
  • 심사 : 2013.03.25
  • 발행 : 2013.04.30
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초록

대장균에서 DNA 결합 단백질로 확인된 DNA-binding Protein from Staved cells (DSP)는 DNA를 보호하는 중요한 기능을 한다는 것을 보여주었다. 이 연구의 목표는 야생형 대장균과 dps 유전자 결손 대장균(${\Delta}dps$ E.coli)의 특성 비교를 통해 여러 종류의 스트레스에 대해 대장균에서 DPS의 기능적 역할을 설명하는 것이다. 다양한 스트레스 상태에서 자외선 흡광도계(UV-spectrophotometer)를 이용하여 야생형 대장균과 dps 유전자 결손 대장균의 세포성장을 측정하였으며, 각각의 대장균 세포 성장 속도를 비교함으로써 우리는 대장균에 존재하는 DPS 단백질의 기능적 역할을 확인하였다. 야생형 대장균에 비해 dps 유전자 결손 대장균은 영양분 결핍, 산성화, 열충격, 다양한 활성산소종 스트레스들에 민감한 현상을 나타내었으며, 이것은 DPS가 다양한 극단적인 스트레스에 중요한 기능을 한다는 것을 제안하였다. 결론적으로 대장균의 DPS는 다양한 환경적인 스트레스로부터 DNA와 강하게 결합하여 유지함으로써 세포를 보호하고 세포성장에 결정적인 기능을 한다는 것을 증명하였다.

The DNA-binding protein from starved cells (DPS), originally identified as a DNA binding protein in Escherichia coli, is known to play an important role in DNA protection. The aim of this study was to evaluate the functional roles of DPS in E. coli against various kinds of external stresses by comparing the properties of wild-type E. coli cells and dps knockout mutant E. coli (${\Delta}dps$) cells. Under various stress conditions, we measured the cell growth of the wild-type E. coli and the dps knockout mutant E. coli (${\Delta}dps$) cells using a UV spectrophotometer. The growth rate of the cells was compared to investigate the functional roles of the DPS protein in E. coli. In comparison to the properties of the wild-type E. coli cells, the dps knockout mutant E. coli (${\Delta}dps$) cells showed highly sensitive phenotypes under various stress conditions, such as heat shock, acidic pH, nutrient deficiency, and different concentrations of reactive oxygen species (ROS), suggesting that DPS plays key roles in E. coli in combating diverse external stresses. The DPS DNA-binding protein in E. coli plays crucial roles in bacterial cell growth and in the protection of the cells from environmental stresses by tightly binding and preserving their DNA molecules.

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