DOI QR코드

DOI QR Code

Salinosporamides A and B Inhibit Proteasome Activity and Delay the Degradation of N-end Rule Model Substrates

  • Shin, Seung Kyun (Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University) ;
  • Bang, Dae In (Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University) ;
  • Choi, Won Hoon (Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University) ;
  • Kim, Seong-Hwan (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Oh, Dong-Chan (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Lee, Min Jae (Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University)
  • Received : 2013.01.14
  • Accepted : 2013.02.13
  • Published : 2013.05.20

Abstract

The proteasome, which is highly evolutionarily conserved, is responsible for the degradation of most short-lived proteins in cells. Small-molecule inhibitors targeting the proteasome's degradative activity have been extensively developed as lead compounds for various human diseases. An exemplified molecule is bortezomib, which was approved by FDA in 2003 for the treatment of multiple myeloma. Here, using transiently and stably expressed N-end rule model substrates in mammalian cells, we evaluated and identified that salinosporamide A and salinosporamide B effectively inhibited the proteasomal degradation. Considering that a variety of proteasome substrates are implicated in the pathogenesis of many diseases, they have the potential to be clinically applicable as therapeutic agents.

Keywords

References

  1. Coux, O.; Tanaka, K.; Goldberg, A. L. Annu. Rev. Biochem. 1996, 65, 801. https://doi.org/10.1146/annurev.bi.65.070196.004101
  2. Lee, M. J.; Lee, B. H.; Hanna, J.; King, R. W.; Finley, D. Mol. Cell. Proteomics 2011, R110.003871. doi: 10.1074/mcp.R110. 003871.
  3. Cardozo, C. Enzyme Protein 1993, 47, 296.
  4. Orlowski, M.; Cardozo, C.; Michaud, C. Biochemistry 1993, 32, 1563. https://doi.org/10.1021/bi00057a022
  5. Ishizawa, J.; Yoshida, S.; Oya, M.; Mizuno, R.; Shinojima, T.; Marumo, K.; Murai, M. Int. J. Oncol. 2004, 25, 697.
  6. Santer, F. R.; Bacher, N.; Moser, B.; Morandell, D.; Ressler, S.; Firth, S. M.; Spoden, G. A.; Sergi, C.; Baxter, R. C.; Jansen-Durr, P.; Zwerschke, W. Cancer Res. 2006, 66, 3024. https://doi.org/10.1158/0008-5472.CAN-05-2013
  7. Brooks, A. D.; Ramirez, T.; Toh, U.; Onksen, J.; Elliott, P. J.; Murphy, W. J.; Sayers, T. J. Ann. N.Y. Acad. Sci. 2005, 1059, 160. https://doi.org/10.1196/annals.1339.042
  8. Inoue, T.; Shiraki, K.; Fuke, H.; Yamanaka, Y.; Miyashita, K.; Yamaguchi, Y.; Yamamoto, N.; Ito, K.; Sugimoto, K.; Nakano, T. Anticancer Drugs 2006, 17, 261. https://doi.org/10.1097/00001813-200603000-00004
  9. Drexler, H. C. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 855. https://doi.org/10.1073/pnas.94.3.855
  10. Pagano, M. FASEB J. 1997, 11, 1067.
  11. Adams, J.; Palombella, V. J.; Sausville, E. A.; Johnson, J.; Destree, A.; Lazarus, D. D.; Maas, J.; Pien, C. S.; Prakash, S.; Elliott, P. J. Cancer Res. 1999, 59, 2615.
  12. Adams, J. Nat. Rev. Cancer 2004, 4, 349. https://doi.org/10.1038/nrc1361
  13. Chauhan, D.; Hideshima, T.; Anderson, K. C. Annu. Rev. Pharmacol. Toxicol. 2005, 45, 465. https://doi.org/10.1146/annurev.pharmtox.45.120403.100037
  14. Feling, R. H.; Buchanan, G. O.; Mincer, T. J.; Kauffman, C. A.; Jensen, P. R.; Fenical, W. Angew. Chem. Int. Ed. 2003, 42, 355. https://doi.org/10.1002/anie.200390115
  15. Kisselev, A. F.; Callard, A.; Goldberg, A. L. J. Biol. Chem. 2006, 281, 8582. https://doi.org/10.1074/jbc.M509043200
  16. Chauhan, D.; Catley, L.; Li, G.; Podar, K.; Hideshima, T.; Velankar, M.; Mitsiades, C.; Mitsiades, N.; Yasui, H.; Letai, A.; Ovaa, H.; Berkers, C.; Nicholson, B.; Chao, T. H.; Neuteboom, S. T.; Richardson, P.; Palladino, M. A.; Anderson, K. C. Cancer Cell 2005, 8, 407. https://doi.org/10.1016/j.ccr.2005.10.013
  17. Hamilton, A. L.; Eder, J. P.; Pavlick, A. C.; Clark, J. W.; Liebes, L.; Garcia-Carbonero, R.; Chachoua, A.; Ryan, D. P.; Soma, V.; Farrell, K.; Kinchla, N.; Boyden, J.; Yee, H.; Zeleniuch-Jacquotte, A.; Wright, J.; Elliott, P.; Adams, J.; Muggia, F. M.; Clin, J. Oncol. 2005, 23, 6107.
  18. Groll, M.; Huber, R.; Potts, B. M. J. Am. Chem. Soc. 2006, 128, 5136. https://doi.org/10.1021/ja058320b
  19. Lee, M. J.; Tasaki, T.; Moroi, K.; An, J. Y.; Kimura, S.; Davydov, I. V.; Kwon, Y. T. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 15030. https://doi.org/10.1073/pnas.0507533102
  20. Lee, M. J.; Kim, D. E.; Zakrzewska, A.; Yoo, Y. D.; Kim, S. H.; Kim, S. T.; Seo, J. W.; Lee, Y. S.; Dorn, G. W.; Oh, U.; Kim, B. Y.; Kwon, Y. T. J. Biol. Chem. 2012, 287, 24043. https://doi.org/10.1074/jbc.M112.364117
  21. Lee, M. J.; Pal, K.; Tasaki, T.; Roy, S.; Jiang, Y.; An, J. Y.; Banerjee, R.; Kwon, Y. T. Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 100. https://doi.org/10.1073/pnas.0708465105