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Assessment of indoor air micro-flora in selected schools

  • Katiyar, Vinita (Department of Respiratory Allergy & Applied Immunology, VP Chest Institute, University of Delhi)
  • 투고 : 2012.11.20
  • 심사 : 2013.03.14
  • 발행 : 2013.03.25

초록

Quantification of viable forms of microbial community (bacteria and fungi) using culture-dependent methods was done in order to characterize the indoor air quality (IAQ). Role of those factors, which may influence the concentration of viable counts of bacteria and fungi, like ventilation, occupancy, outdoor concentration and environmental parameters (temperature and relative humidity) were also determined. Volumetric-infiltration sampling technique was employed to collect air samples both inside and outside the schools. As regard of measurements of airborne viable culturable microflora of schools during one academic year, the level of TVMCs in school buildings was ranged between 803-5368 cfu/$m^3$. Viable counts of bacteria (VBCs) were constituted 63.7% of the mean total viable microbial counts where as viable counts of fungi (VFCs) formed 36.3% of the total. Mean a total viable microbial count (TVMCs) in three schools was 2491 cfu/$m^3$. Outdoor level of TVMCs was varied from 736-5855 cfu/$m^3$. Maximum and minimum VBCs were 3678-286 cfu/m3 respectively. Culturable fungal counts were ranged from 268-2089 cfu/$m^3$ in three schools. Significant positive correlation (p < 0.01) was indicated that indoor concentration of viable community reliant upon outdoor concentration. Temperature seemed to have a large effect (p < 0.05, p < 0.01) on the concentration of viable culturable microbial community rather than relative humidity. Consistent with the analysis and findings, the concentration of viable cultural counts of bacteria and fungi found indoors, were of several orders of magnitude, depending upon the potential of local, spatial and temporal factors, IO ratio appeared as a crucial indicator to identify the source of microbial contaminants.

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참고문헌

  1. Adhikari, A., Sen, M.M., Bhattacharya-Gupta, S. and Chanad, S. (2004a), "Airborne viable, non-viable and allergenic fungi in a rural agricultural area of India: a 2-year study at five outdoor sampling stations", Sci. Total Environ., 326(1-3), 123-141. https://doi.org/10.1016/j.scitotenv.2003.12.007
  2. Adhikari, A., Sen, M.M., Bhattacharya-Gupta, S. and Chanad, S. (2004b), "Volumetric assessment of airborne fungi in two sections of rural indoor dairy cattle shed", Environ. Inter., 29(8), 1071-1078. https://doi.org/10.1016/S0160-4120(03)00103-X
  3. Adhikari, A., Reponen, T., Grinshpun, S.A., Martuzevicius, D. and LeMasters, G. (2005), "Correlation of ambient inhalable bioaerosols with particulate matter and ozone: A two-year study", Environ. Pollut., 140, 16-28.
  4. Albinas, L., Krikstaponis, A. and Sveistyte, L. (2004), "Airborne fungi in industrial environments-potential agents of respiratory diseases", Ann. Agric. Environ. Med., 11(1), 19-25.
  5. American Conference of Governmental Industrial Hygienists (ACGIH) (1989), Guidelines for the assessment of bioaerosols in the indoor environment, Cincinnati, OH, USA.
  6. Pasanen, A.L., Kujanpaa, L., Pasanen, P., Kalliokoski, P. and Blomquist, G. (2004), "Culturable and total Fungi in dust accumulated in air ducts in single-family houses", Indoor Air., 7(2), 121-127.
  7. Aprea, C., Strambi, M., Novelli, M.T., Lunghini, L. and Bozzi, N. (2000), "Biologic monitoring of exposure to organophosphorus pesticides", Environ. Health Persp., 108, 521-525. https://doi.org/10.1289/ehp.00108521
  8. Augustowska, M. and Dutkiewicz, J. (2006), "Variability of airborne micro flora in a hospital ward within a period of one year", Ann. Agric. Environ. Med., 13, 99-106.
  9. Barnes, C.S., Kennedy, K., Gard, L., Forrest, E., Johnson, L., Pacheco, F., Hu, F., Amado, M. and Portnoy, J.M. (2008), "The impact of home cleaning on quality of life for homes with asthmatic children", Allergy Asthma Proc., 29(2), 197-204. https://doi.org/10.2500/aap.2008.29.3099
  10. Bakutis, B., Monstviliene, E. and Januskeviciene, G. (2004), "Analyses of airborne contamination with bacteria, endotoxins and dust in livestock barns and poultry houses", Acta Vet. BRNO, 73, 283-289. https://doi.org/10.2754/avb200473020283
  11. Bonetta, S., Bonetta, S., Mosso, S., Sampo, S. and Carraro, E. (2009), "Assessment of microbiological indoor air quality in an Italian office building equipped with an HVAC system", Environ. Monit., 161(1-4), 473-483.
  12. Bouillard, L., Michel, O., Dramaix, M. and Devleeschouwer, M. (2005), "Bacterial contamination of indoor air, surfaces, and settled dust, and related dust endotoxin concentrations in healthy office buildings", Ann. Agric. Environ. Med., 12, 187-192.
  13. Brooks, S.M., Spaul, W. and McCluskey, J.D. (2005), "The spectrum of building-related airway disorders: difficulty in retrospectively diagnosing building-related asthma", Chest., 128(3), 1720-1727. https://doi.org/10.1378/chest.128.3.1720
  14. CEC (1994), Report No. 12: Biological particles in indoor environments, Commission of the European Communities, Luxembourg.
  15. Curtis, L., Lieberman, A., Stark, M., Rea, W. and Vetter, M. (2004), "Adverse health effects of indoor molds", J. Nutr. Environ. Med., 14(3) 261-274.
  16. Daisey, J.M., Angell, W.J. and Apte, M.G. (2003), "Indoor air quality, ventilation and health symptoms in schools: An analysis of existing information", Indoor Air., 13(1), 53-64. https://doi.org/10.1034/j.1600-0668.2003.00153.x
  17. Ebbehoj, N.E., Meyer, H.W., Wurtz, H., Suadicani, P., Valbjorn, O., Sigsgaard, T. and Gyntelberg, F. (2005), (Members of working Group under the Danish mold in Building program (DMAMIB)), Molds in floor dust, building-related symptoms, and lung function among male and female school teachers, Indoor Air., 15(Suppl. 10), 7-16. https://doi.org/10.1111/j.1600-0668.2005.00352.x
  18. Giovannangelo, M., Nordling, E., Gehring, U., Oldenwening, M., Bellander, T., Heinrich, J., Hoek, G. and Brunekreef, B. (2007), "Variation of biocontaminant levels within and between homes - the AIRALLERG study", J. Expo. Sci. Environ. Epidemiol., 17(2), 134-140. https://doi.org/10.1038/sj.jes.7500482
  19. Godwin, C. and Batterman S. (2007), "Indoor air quality in Michigan schools", Indoor Air., 17(2), 109-121. https://doi.org/10.1111/j.1600-0668.2006.00459.x
  20. Gorny, R.L. and Dutkiewicz J. (2002), "Bacterial and fungal aerosol in indoor environment in central and eastern European countries, Ann. Agric. Environ. Med., 917-923.
  21. Hamilos, D.L. (2010), "Allergic fungal rhinitis and rhinosinusitis", Proc. Am. Thorac. Soc., 7(3), 245-252. https://doi.org/10.1513/pats.200909-098AL
  22. Inal, A., Kendirli, S.G., Yilmaz, M., Altintas, D.U., Karakoc, G.B. and Erdogan, S. (2008). "Indices of lower airway inflammation in children monosensitized to house dust mite after nasal allergen challenge", Allergy., 63(10), 1345-1351. https://doi.org/10.1111/j.1398-9995.2008.01694.x
  23. Jensen, P.A. and Schafer, M.P. (1998), "Sampling and characterization of bioaerosols", NIOSH Manual of Analytical Methods, 82-112.
  24. Jo, W.K. and Seo, Y.J. (2005), "Indoor and outdoor bioaerosol levels at recreation facilities, elementary schools, and homes", Chemosphere., 61(11), 1570-1579. https://doi.org/10.1016/j.chemosphere.2005.04.103
  25. Kalogerakis, N., Paschali, D., Lekaditis, V., Pantidou, A., Eleftheriadis, K. and Lazaridis, M. (2005), Indoor air quality-bioaerosol measurements in domestic and offices premises", Aerosol Sci., 36, 751-761. https://doi.org/10.1016/j.jaerosci.2005.02.004
  26. Karvala, K., Nordman, H., Luukkonen, R., Nykyri, E., Lappalainen, S., Hannu, T. and Toskala, E. (2008), "Occupational rhinitis in damp and moldy workplaces", Am. J. Rhinol., 22(5), 57- 462.
  27. Kate, T.H.D., Michael, L.M., Harriet, A.B. and Noah, S.S. (2002), "Effect of sampling teme on the culturability of airborne fungi and bacteria sampled by filtration", Ann. Occup. Hyg., 46(1)113-118.
  28. Kelman, B.J., Robbins, C.A., Swenson, L.J. and Hardin, B.D. (2004), "Risk from inhaled mycotoxins in indoor office and residential environments", Int. J. Toxicol., 23(1), 3-10. https://doi.org/10.1080/762483054
  29. Kim, J.L., Elfman, L., Mi, Y., Wieslander, G., Smedje, G. and Norbäck D. (2007), "Indoor molds, bacteria, microbial volatile organic compounds and plasticizers in schools-associations with asthma and respiratory symptoms in pupils", Indoor Air., 17(2), 153-163. https://doi.org/10.1111/j.1600-0668.2006.00466.x
  30. Lignell, U., Meklin, T., Putus, T., Rintala, H., Vepsalainen, A., Kalliokoski, P. and Nevalainen, A. (2007), "Effects of moisture damage and renovation on microbial conditions and pupils' health in two schools-a longitudinal analysis of five years", J. Environ. Monit., 9(3), 225-233. https://doi.org/10.1039/b615459j
  31. Lucette, B., Michel, O., Dramaix, M. and Devleeschouwer, M. (2005), "Bacterial contamination of indoor air, surfaces and settled dust and related dust endotoxin concentrations in healthy office building", Ann. Agric. Environ. Med., 12, 187-192.
  32. Madani, Y., Barlow, A. and Taher F. (2010), "Severe asthma with fungal sensitization: a case report and review of literature", J. Asthma., 47(1), 2-6. https://doi.org/10.3109/02770900903362684
  33. Matkovic, K., Vucemilo, M., Vinkovic, B., Seol, B., Pavicic, Z., Tofant, A. and Matkovic, S. (2006), "Effect of microclimate on bacterial count and airborne emission from dairy barns the environment", Ann. Agric. Environ. Med., 13, 349-354.
  34. Meklin, T., Reponen, M.T., Koponen, V., Husman, T., Hyvarinen, A. and Nevalainen, A. (2002), "Size distributions of airborne microbes in moisture-damaged and reference school buildings of two construction types", Atmos. Environ., 36, 6031-6039. https://doi.org/10.1016/S1352-2310(02)00769-0
  35. Mendell, M.J. and Heath G.A. (2005), "Do indoor pollutants and thermal conditions in schools influence student performance? A critical review of the literature", Indoor Air., 15(1), 27-52.
  36. Meyer, H.W., Wurtz H., Suadicani, P., Valbjorn, O., Sigsgaard, T. and Gyntelberg, F. (2005), (Members of working Group under the Danish mold in Building program (DMAMIB)), "Molds in floor dust and building related symptoms among adolescent school children: a problem for boys only?", Indoor Air., 15 Suppl., 10, 17-24. https://doi.org/10.1111/j.1600-0668.2005.00356.x
  37. Mota, L.C., Gibbs, S.G., Green, C.F., Flores, C.M., Tarwater, P.M. and Ortiz, M. (2008), "Seasonal fine and coarse culturable fungal constituents and concentrations from indoor and outdoor air samples taken from an arid environment", J. Occup. Environ. Hyg., 5(8), 511-518. https://doi.org/10.1080/15459620802208636
  38. Nielsen, P.V. (2009), "Control of airborne infectious diseases in ventilated spaces", R. Soc. Interface., 6(Suppl. 6), 47-55.
  39. Niemeier, R.T., Sivasubramani, S.K., Reponen, T. and Grinshpun, S.A. (2006), "Assessment of fungal contamination in moldy homes: Comparison of different methods", J. Occup. Environ. Hyg., 3(95), 262-273. https://doi.org/10.1080/15459620600637333
  40. Northcut, J.K., Jones, D.R. and Musgrove, M.T. (2004), "Airborne microorganisms during the commercial production and processing of Japanese Quail", Int. J. Poultry Sci., 3(4), 242- 247. https://doi.org/10.3923/ijps.2004.242.247
  41. Pantelic, J., Sze-To G.N., Tham, K.W., Chao C.Y. and Khoo Y.C. (2009), "Personalized ventilation as a control measure for airborne transmissible disease spread", J. R. Soc. Interface., 6(Suppl. 6), 715-726. https://doi.org/10.1098/rsif.2009.0311.focus
  42. Park, J.H., Cox-Ganser, J., Rao, C. and Kreiss, K. (2006), "Fungal and endotoxin measurements in dust associated with respiratory symptoms in a water-damaged office building", Indoor Air., 16(3), 192-203. https://doi.org/10.1111/j.1600-0668.2005.00415.x
  43. Patovirta, R.L., Husman, T., Haverinen, U., Vahteristo, M., Uitti, J. A., Tukiainen, H. and Nevalainen, A. (2004), "The remediation of mold damaged school--a three-year follow-up study on teachers' health", Cent. Eur. J. Public Health., 12(1), 36-42.
  44. Pongracic, J.A., O'Connor, G.T., Muilenberg, M.L., Vaughn, B., Gold, D.R., Kattan, M., Morgan, W.J., Gruchalla, R.S., Smartt, E. and Mitchell, H.E. (2010), "Differential effects of outdoor versus indoor fungal spores on asthma morbidity in inner-city children". J. Allergy. Clin. Immunol., 125(3), 593-599. https://doi.org/10.1016/j.jaci.2009.10.036
  45. Prazmo, Z., Dutkiewicz, J., Skorska, C., Sitkowska, J. and Cholewa, G. (2003), "Exposure to airborne Grm-negative bacteria, dust and endotoxin in paper factories", Ann. Agric. Environ. Med., 10(1), 93-100.
  46. Razek, S.A., Mohsen, M.B.A. and Abdelmonem, W.M., Indoor Air Biocontaminants and Suspended Dust Levels. SEED Journal. http://www.seed.slb.com/ssjournal.aspx?id=31794.
  47. Ren, P., Jankun, M.T. and Leaderer, B.P. (1999), "Comparison of seasonal prevalence in indoor and outdoor air and in house dusts of dwellings in one Northeast American county", J. Expo. Anal. Environ. Epidemiol., 9(6), 560-568. https://doi.org/10.1038/sj.jea.7500061
  48. Rogers, C.A. (2003), "Indoor fungal exposure", Immunol. Allergy Clin. North Am., 23(3), 501-518. https://doi.org/10.1016/S0889-8561(03)00061-4
  49. Santilli, J. (2002), "Health effects of mold exposure in public schools", Curr. Allergy Asthma Rep., 2(6), 460-467. https://doi.org/10.1007/s11882-002-0085-z
  50. Santilli, J. and Rockwell, W. (2003), "Fungal contamination of elementary schools: a new environmental hazard", Ann. Allergy Asthma Immunol., 90(2), 203-208. https://doi.org/10.1016/S1081-1206(10)62142-4
  51. Smedge, G., Norback, D. and Edling, C. (1997), "Subjective indoor air quality in school in relation to exposure", Indoor Air., 7, 143-150. https://doi.org/10.1111/j.1600-0668.1997.00009.x
  52. Smedge, G. and Norback, D. (2001), "Irritants and allergens at schools in relation to furnishing and cleaning", Indoor Air., 11(2), 127-133. https://doi.org/10.1034/j.1600-0668.2001.110207.x
  53. Stetzenbach, L.D., Buttner, M.P. and Cruz, P. (2004), "Detection and enumeration of airborne biocontaminants", Curr. Opin. Biotechnol., 15(3), 170-174. https://doi.org/10.1016/j.copbio.2004.04.009
  54. Sundell, J. (2004), "On the history of indoor air quality and health", Indoor Air., 14(Suppl. 7), 51-58.
  55. Taekhee, L., Sergey, A.G., Martuzevicius, D., Adhikari, A., Carlos M.C. and Reponen, T. (2006), "Culturability and concentration of indoor and outdoor airborne fungi in six stage-family homes", Atmos. Environ., 40(16), 2902-2910. https://doi.org/10.1016/j.atmosenv.2006.01.011
  56. Tsai, M.Y. and Liu, H.M. (2009), "Exposure to culturable airborne bioaerosol during noodle manufacturing in central Taiwan", Sci. Total Environ., 407(5), 1536-1546. https://doi.org/10.1016/j.scitotenv.2008.10.029
  57. World Health Organization (WHO) (1990), "Indoor Air quality: Biological Contaminants: European Series Number No. 31", Copenhagen, WHO Regional Publication.
  58. Zhao, Z., Sebastian, A., Larsson, L., Wang, Z., Zhang, Z. and Norbäck, D. (2008), "Asthmatic symptoms among pupils in relation to microbial dust exposure in schools in Taiyuan, China", Pediatr. Allergy Immunol., 19(5), 455-465. https://doi.org/10.1111/j.1399-3038.2007.00664.x
  59. Zhu, H., Phelan, P.E., Duan, T., Raupp, G.B., Fernando, H.J.S. and Che, F. (2003), "Experimental study of indoor and outdoor airborne bacterial concentrations in Tempe, Arizona, USA", Aerobiologia., 19, 201-211. https://doi.org/10.1023/B:AERO.0000006571.23160.8a
  60. Zilma, G.N., Martins, A.S., Altoe, A.L., Nishikawa, M.M., Leite, M.O., Aguiar, P.F. and Fracalanzza, S.E.L. (2005), "Indoor air microbiological evaluation of offices, hospitals, industries, and shipping centers", Mem. Inst. Oswaldo Cruz., 100(4), 351-357. https://doi.org/10.1590/S0074-02762005000400003

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