Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of Flavonoids from the Roots of Brassica rapa ssp.

순무(Brassica rapa ssp.) 뿌리로부터 flavonoid의 분리 및 동정

  • Jeong, Rak-Hun (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Wu, Qian (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Cho, Jin-Gyeong (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Dae-Young (Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA) ;
  • Shrestha, Sabina (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Min-Ho (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Kyung-Tae (Department of Biochemistry, College of Pharmacy, Kyung Hee University) ;
  • Choi, Myung-Sook (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Jeong, Tae-Sook (National Research Laboratory of Lipid Metabolism & Atherosclerosis, KRIBB) ;
  • Ahn, Eun-Mi (Department of Herbal Foodceutical Science, DaeguHaany University) ;
  • Chung, Hae-Gon (Ganghwa Agricultural R&D Center) ;
  • Rho, Yeong-Deok (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Baek, Nam-In (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University)
  • Received : 2012.09.10
  • Accepted : 2012.11.02
  • Published : 2013.03.31
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Abstract

The roots of Brassica rapa ssp. were extracted with 95% aqueous ethanol and the concentrated extracts were partitioned using ethyl acetate (EtOAc), n-butyl alcohol and $H_2O$, successively. From the EtOAc fraction, five flavonoids were isolated through repeated silica gel and octadecyl silica gel (ODS) column chromatography (c.c.). Based on NMR, mass spectrometry (MS) and IR spectroscopic data, the chemical structures of the compounds were determined to be licochalcone A (1), 4,4'-dihydroxy-3'-methoxychalcone (2), liquirtigenin (3), liquiritin (4), and isoliquiritin (5). This is the first report of these compounds isolated from the root of this plant.

순무뿌리(Brassica rapassp)를 실온에서 95% ethanol 수용액으로 추출하고 이 추출물을 ethyl acetate (EtOAc)분획, n-butyl alcohol 분획 및 $H_2O$ 분획으로 나누었다. EtOAc분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography를 반복실시 하여 5종의 flavonoid를 분리하였다. NMR, IR 및 MS data를 해석하여 각각 licochalcone A (1), 4,4'-dihydroxy-3'-methoxychalcone (2), liquiritigenin (3), liquiritin (4), isoliquiritin (5)으로 구조동정하였다. 이들 화합물들은 순무뿌리에서는 처음으로 분리되었다.

Keywords

References

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