Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Molecular Epidemiology of Legionella pneumophila Isolated from Water Supply Systems in Seoul, Korea

서울시 수계시설에서 분리된 Legionella pneumophila의 분자역학적 특성

  • Jeon, Su Jin (Division of Infectious disease, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jung, Ji Hun (Division of Infectious disease, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Seung, Hyun Jung (Division of Infectious disease, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Chang Kyu (Division of Infectious disease, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jin, Young Hee (Division of Infectious disease, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Oh, Young Hee (Division of Infectious disease, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Choi, Sung Min (Division of Infectious disease, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Chae, Young Zoo (Division of Infectious disease, Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 전수진 (서울시보건환경연구원 감염병검사팀) ;
  • 정지헌 (서울시보건환경연구원 감염병검사팀) ;
  • 승현정 (서울시보건환경연구원 감염병검사팀) ;
  • 김창규 (서울시보건환경연구원 감염병검사팀) ;
  • 진영희 (서울시보건환경연구원 감염병검사팀) ;
  • 오영희 (서울시보건환경연구원 감염병검사팀) ;
  • 최성민 (서울시보건환경연구원 감염병검사팀) ;
  • 채영주 (서울시보건환경연구원 감염병검사팀)
  • Received : 2013.02.04
  • Accepted : 2013.03.07
  • Published : 2013.04.30
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Abstract

Objectives: The genus Legionella is common in aquatic environments. Some species of Legionella are recognized as potential opportunistic pathogens for human, notably Legionella pneumophila that causes, Legionellosis. Thus, we investigated the contamination of Legionella pneumophila on water supply systems in Seoul, including cooling towers, public baths, hospitals and fountains. Methods: The existence of 16S rRNA and mip gene of L. pneumophila was confirmed in the genome of the isolated strains by PCR. Results: During the summer season of 2010 and 2011, Legionella pneumophila were detected from 163 samples (21.1%) out of 772 samples collected. Among the 163 strains of L. pneumophila, eighty one isolates belonged to serogroup 1 (57.4%), 23 isolates were serogroup 5 (16.3%), 21 isolates were serogroup 6 (14.9%), 8 isolates were serogroup 2 (5.79%), and 8 isolates were identified in serogroup 3 (5.7%). Through PFGE (pulsed-field gel electrophoresis) analysis using Sfi I, genetic types of L. pneumophila were classified into five (A to E) patterns by the band similarity with excess of 70% from public baths. Conclusions: The PFGE patterns of the serotypes showed a tendency for diversity of L. pneumophila. Our results suggest the existence of serological and genetic diversity among the L. pneumophila isolates.

Keywords

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