Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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The Spermatogenic Effect of Yacon Extract and Its Constituents and Their Inhibition Effect of Testosterone Metabolism

  • Park, Jeong Sook (College of Pharmacy, Chungbuk National University) ;
  • Han, Kun (College of Pharmacy, Chungbuk National University)
  • Received : 2012.11.27
  • Accepted : 2013.03.12
  • Published : 2013.03.31
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Abstract

We screened the pharmacological effects of a 50% ethanol extract of Yacon tubers and leaves on spermatogenesis in rats. As a result, we found that Yacon tuber extracts increased sperm number and serum testosterone level in rats. It has been reported that the crude extract of Yacon tubers and leaves contain phenolic acids, such as, chlorogenic acid, ferulic acid and caffeic acid by HPLC/MS analysis. We were interested in the contributions made by phenolic acid, particularly chlorogenic acid of Yacon tuber extract to the spermatogenic activity. After administering Yacon tuber extract or chlorogenic acid to rats for 5 weeks, numbers of sperm in epididymis were increased by 34% and 20%, respectively. We also administered ferulic acid, which has been reported to be a metabolite of chlorogenic acid and a constituent of Yacon tuber extract to investigate its spermatogenic activity in rats. Yacon tuber extract and ferulic acid increased sperm numbers by 43% and 37%, respectively. And, Yacon tuber extract, and chlorogenic acid showed significantly inhibition effect of testoeterone degradation in rat liver homogenate. We considered that the spermatogenic effect of Yacon tuber extract might be related to phenolic compounds and their inhibitory effect of testosterone degradation. Yacon showed the possibility as ameliorable agents of infertility by sperm deficiency and late onset hypogonadism syndrome with low level of testosterone.

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References

  1. Abdel-Magied, E. M., Abdel-Rahman H. A. and Harraz, F. M. (2001) The effect of aqueous extracts of Cynomorium coccineum and Withania somnifera on testicular development in immature Wistar rats. J. Ethnopharmacol. 75, 1-4. https://doi.org/10.1016/S0378-8741(00)00348-2
  2. Ahmad, M. K., Mahdi, A. A., Shukla, K. K., Islam, N., Jaiswar, S. P. and Ahmad, S. (2008) Effect of Mucuna pruriens on semen profile and biochemical parameters in seminal plasma of infertile men. Fertil. Steril. 90, 627-635. https://doi.org/10.1016/j.fertnstert.2007.07.1314
  3. Archer, S. (1993) Measurement of nitric oxide in biological models. FASEB J. 7, 349-360. https://doi.org/10.1096/fasebj.7.2.8440411
  4. Aybar, M. J., Sanchez Riera, A. N., Grau, A. and Sanchez, S. S. (2001) Hypoglycemic effect of the water extract of Smallanthus sonchifolius (yacon) leaves in normal and diabetic rats. J. Ethnopharmacol. 74, 125-132. https://doi.org/10.1016/S0378-8741(00)00351-2
  5. Barcellona, C. S., Cabrera, W. M., Honore, S. M., Mercado, M. I., Sanchez, S. S. and Genta, S. B. (2012) Safety assessment of aqueous extract from leaf Smallanthus sonchifolius and its main active lactone, enhydrin. J. Ethnopharmacol. 144, 362-370. https://doi.org/10.1016/j.jep.2012.09.021
  6. Bassil, N., Alkaade, S. and Morley, J. E. (2009) The benefits and risks of testosterone replacement therapy. Ther. Clin. Risk Manag. 5, 427-448.
  7. Brand-Williams, W., Cuvelier, M. E. and Berset, C. (1995) Use of a free radical method to evaluate antioxidative activity. Food Sci. Technol. 28, 25-30.
  8. Carlsen, E. L., Giwercmn, A., Keiding, N., and Skakkebaek, E. (1992) Evidence for decreasing quality of semen during past 50 years. Br. Med. J. 305, 609-613. https://doi.org/10.1136/bmj.305.6854.609
  9. Comhaire, F. H. (2000) Andropause: hormone replacement therapy in the ageing male. Eur. Urol. 38, 655-662.
  10. Ding, E. L., Song, Y., Malik, V. S. and Liu, S. (2006) Sex differences of endogenous sex hormones and risk of type 2 diabetes: a systematic review and meta-analysis. JAMA 295, 1288-1299. https://doi.org/10.1001/jama.295.11.1288
  11. Fraga, C. G., Motchnik, P. A., Shigenaga, M.K., Helbock, H. J., Jacob, R. A. and Ames, B. N. (1991) Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proc. Natl. Acad. Sci. U.S.A. 88, 11003-11006. https://doi.org/10.1073/pnas.88.24.11003
  12. Geva, E., Bartoov, B., Zabludovsky, N., Lessing, J. B., Lerner-Geva, L. and Amit, A.(1996) The effect of antioxidant treatment on human spermatozoa and fertilization rate in an in vitro fertilization program. Fertil.Steril. 66, 430-434. https://doi.org/10.1016/S0015-0282(16)58514-8
  13. Goto, K., Fukai, K., Hikida, J., Nanjo, F. and Hara, Y. (1995) Isolation and structural analysis of oligosaccharides from yacon (Polymnia sonchifolia). Biosci. Biotechnol. Biochem. 59, 2346-2347. https://doi.org/10.1271/bbb.59.2346
  14. Harman, S. M., Metter, E. J., Tobin. J. D., Pearson. J. and Blackman, M. R. (2001) Longitudinal effects of aging on serum total and free testosterone levels in healthy men. Baltimore Longitudinal Study of Aging. J. Clin. Endocrinol. Metab. 86, 724-731. https://doi.org/10.1210/jc.86.2.724
  15. Kaneto, H., Fujii, J., Seo, H. G., Suzuki, K., Matsuoka, T., Nakamura, M., Tatsumi, H., Yamazaki, Y., Kamada, T. and Taniguchi, N. (1995) Apoptotic cell death triggered by nitric oxide in pancreatic ${\beta}$-cells. Diabetes 44, 733-738. https://doi.org/10.2337/diabetes.44.7.733
  16. Khaw, K. T., Dowsett, M., Folkerd, E., Bingham, S., Wareham, N., Luben, R., Welch, A. and Day, N. (2007) Endogenous testosterone and mortality due to all causes, cardiovascular disease and cancer in men: European prospective investigation into cancer in Norfolk (EPIC-Norfolk) Prospective Population Study. Circulation 116, 2694-2701. https://doi.org/10.1161/CIRCULATIONAHA.107.719005
  17. Latchoumycandane, C., Chitra., K. C. and Mathur, P. P. (2003) 2,3,7, 8,-Tetrachlorodibenzo-p-dioxin (TCDD) induces oxidative stress in the epididymis sperm of adult rats. Arch. Toxicol. 77, 280-284. https://doi.org/10.1007/s00204-003-0439-x
  18. Latchoumycandane, C. and Mathur, P. P. (2002) Effects of vitamine on reactive oxygen species-mediated 2,3,7,8,-Tetrachlorodibenzo-p-dioxin toxicity in rat testis. J. Appl. Toxicol. 22, 345-351. https://doi.org/10.1002/jat.866
  19. Lewin, A. and Lavon, H. (1997) The effect of coenzyme Q10 on sperm motility and function. Mol. Aspects Med. 18 Suppl, S213-219. https://doi.org/10.1016/S0098-2997(97)00036-8
  20. Liu, J., Kurashiki, K., Shimizu, K. and Kondo, R. (2006) Structure-activity relationship for inhibition of 5alpah-reductase by triterpenoids isolated from Ganoderma lucidum. Bioorg. Med. Chem. 14, 8654-8660. https://doi.org/10.1016/j.bmc.2006.08.018
  21. Lowenstein, C. J., Dinerman J. L. and Snyder, S. H. (1994) Nitric oxide: a physiologic messenger. Ann. Intern. Med. 210, 227-237.
  22. Lowry, O. H., Rosbrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin Phenol reagent. J. Biol. Chem. 193, 265-275.
  23. Matsuda, H., Sato, N., Yamazaki, M., Naruto, S. and Kubo, M. (2001) Testosterone 5alpah-reductase Inhibitory active constituents from Anemarrhenae Rhizoma. Biol. Pharm. Bull. 24, 586-587. https://doi.org/10.1248/bpb.24.586
  24. Moncada, S., Palmer, R. M. J. and Higgs, E. A. (1991) Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol. Rev. 43, 109-142.
  25. Ohyama, T., Ito, O., Yasuyoshi, S., Ikarashi, T., Minamisawa, K., Kuboda, M., Tsuhihashi, T. and Asami, T. (1990) Composition of storage carbohydrates in tubers of yacon (Polymnia sonchifolia). Soil Sci. Plant Nutr. 36, 167-171. https://doi.org/10.1080/00380768.1990.10415724
  26. Park, J. S., Hwang, S. Y., Hwang, B. Y. and Han, K. (2008) The spermatogenic effect of 50% ethanol extracts of Yacon and Its ameliorative effect against 2,3,7,8-tetrachlorodibenzo-p-dioxin induced testicular toxicity in the rat. Nat. Prod. Sci. 14, 73-80.
  27. Park, J. S., Hwang, S. Y. and Han, K. (2009) The spermatogenic effect of 50% ethanol extracts of Yacon in healthy male volunteers. Yakhak Hoeji 53, 250-258.
  28. Rolf, C., Cooper, T. G., Yeung, C. H. and Nieschlag, E. (1999) Antioxidant treatment of patients with asthenozoospermia or moderate oligoasthenozoospermia with high-dose vitamin C and vitamin E: a randomized, placebo-controlled, double-blind study. Hum. Reprod. 14, 1028-1033. https://doi.org/10.1093/humrep/14.4.1028
  29. Sandler, B. and Faragher, B. (1984) Treatment of oligospermia with vitamin B12. Infertility 7, 133-138.
  30. Schachter, A., Goldman, J. A. and Zukerman, Z. (1973) Treatment of oligospermia with the amino acid arginine. J .Urol. 110, 311-313. https://doi.org/10.1016/S0022-5347(17)60199-X
  31. Scott, R., MacPherson, A. and Yates, R. W. S. (1998) The effect of oral selenium supplementation on human sperm motility. Br. J. Urol. 82, 76-80. https://doi.org/10.1046/j.1464-410x.1998.00683.x
  32. Shabsigh, R., Crawford, E. D., Nehra, A. and Slawin, K. M. (2009) Testosterone theraphy in hypogonadal men and potential prostate cancer risk: a systemic review. Int. J. Impot.Res. 21, 9-23. https://doi.org/10.1038/ijir.2008.31
  33. Sharpe, R. (1994) The physiology of reproduction (E. Knobil, J. Neill, Eds.), 2nd ed. pp. 1364-1434. Raven Press, New York.
  34. Singleton, V. L. and Rossi, J. A. Jr. (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144-158.
  35. Simonovska, B., Vovk, I., Andrensek, S., Valentova, K. and Ulrichova, J. (2003) Investigation of phenolic acids in yacon (Smallanthus sonchifolius) leaves and tubers. J. Chromatogr. A 1016, 89-98. https://doi.org/10.1016/S0021-9673(03)01183-X
  36. Stadler, J., Billiar T. R., Curran, R. D., Stuehr, D. J., Ochoa, J. B. and Simons, R. L. (1991) Effect of exogenous and endogenous nitric oxide on mitochondrial respiration of rat hepatocytes. Am. J. Physiol. 260, C910-916. https://doi.org/10.1152/ajpcell.1991.260.5.C910
  37. Toth, G. P., Zenick, H. and Smith, M. K. (1989) Effects of epichlorohydrin on male and female reproduction in Long-Evans rats. Fundam. Appl. Toxicol. 13, 16-25. https://doi.org/10.1016/0272-0590(89)90303-5
  38. Valentova, K., Cvak, L., Muck, A., Ulrichova, J. and Simanek, V. (2003) Antioxidant activities of extracts from the leaves of Smallanthus sonchifolius. Eur. J. Nutr. 42, 61-66. https://doi.org/10.1007/s00394-003-0402-x
  39. Valentova, K., Sersen, F. and Ulrichova, J. (2005) Radical scavenging and anti-lipoperoxidative activity of Smallanthus sonchifolius leaf extracts. J. Agric. Food Chem. 53, 5577-5582. https://doi.org/10.1021/jf050403o
  40. Yan, X., Suzuki, M., Ohnishi-Kameyama, M., Sada, Y., Nakanishi, T. and Nagata, T. (1999) Extraction and identification of antioxidants in roots of yacon (Smallanthus sonchifolius). J. Agric. Food Chem. 47, 4711-4713. https://doi.org/10.1021/jf981305o
  41. Zitzmann, M. (2009) Testosterone deficiency, insulin resistance and the metabolic syndrome. Nat. Rev. Endocrinol. 5, 673-681. https://doi.org/10.1038/nrendo.2009.212

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