Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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A Methanol Extract of Adansonia digitata L. Leaves Inhibits Pro-Inflammatory iNOS Possibly via the Inhibition of NF-κB Activation

  • Ayele, Yihunie (Food Science and Nutrition Program, Addis Ababa University) ;
  • Kim, Jung-Ah (Department of Food Science and Nutrition, Soonchunhyang University) ;
  • Park, Eunhee (Department of Food Science and Nutrition, Soonchunhyang University) ;
  • Kim, Ye-Jung (Department of Food Science and Nutrition, Soonchunhyang University) ;
  • Retta, Negussie (Food Science and Nutrition Program, Addis Ababa University) ;
  • Dessie, Gulelat (Food Science and Nutrition Program, Addis Ababa University) ;
  • Rhee, Sang-Ki (Department of SCH-BioPharm Human Resources Development Center) ;
  • Koh, Kwangoh (Department of Chemistry, Soonchunhyang University) ;
  • Nam, Kung-Woo (Department of SCH-BioPharm Human Resources Development Center) ;
  • Kim, Hee Seon (Department of Food Science and Nutrition, Soonchunhyang University)
  • Received : 2012.12.14
  • Accepted : 2013.02.19
  • Published : 2013.03.31
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Abstract

This study examined the total polyphenol content of eight wild edible plants from Ethiopia and their effect on NO production in Raw264.7 cells. Owing to its relatively high polyphenol concentration and inhibition of NO production, the methanol extract of Adansonia digitata L. leaf (MEAD) was subjected to detailed evaluation of its antioxidant and anti-inflammatory effects. Antioxidant effects were assessed by measuring free-radical-scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and oxygen-radical-absorbance capacity (ORAC) assays, while anti-inflammatory effects were assessed by measuring inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. In the ORAC assay, MEAD was 10.2 times more potent than vitamin C at eliminating peroxyl radicals. In DPPH assay, MEAD also showed a strong ROS scavenging effect. MEAD significantly inhibited iNOS activity ($IC_{50}=28.6{\mu}g/ml$) of LPS-stimulated Raw264.7 cells. We also investigated the relationship between iNOS expression and nuclear factor kappa B (NF-${\kappa}B$) activation. MEAD inhibited $I{\kappa}B{\alpha}$ degradation and NF-${\kappa}B$ translocation from the cytosol to the nucleus in LPS-induced RAW264.7 cells without significant cytotoxic effects, as confirmed by MTT assay. These results suggest that MEAD inhibits anti-inflammatory iNOS expression, which might be related to the elimination of peroxyl radicals and thus the inhibition of $I{\kappa}B{\alpha}$-mediated NF-${\kappa}B$ signal transduction.

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References

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