생명과학회지 (Journal of Life Science)

  • 제23권3호
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  • Pages.383-388
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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감꼭지 추출물과 분획물의 미백효과

Whitening Effect of Extracts and Fractions from Diospyros kaki calyx

  • Hwang, Ju-Young (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Park, Tae-Soon (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Son, Jun-Ho (Korea Promotion Institute for Traditional Medicine Industry)
  • 투고 : 2012.12.04
  • 심사 : 2012.03.11
  • 발행 : 2013.03.30
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초록

본 연구는 감꼭지 추출물과 용매별 분획물에 대한 미백효과를 측정하였다. 감꼭지를 70% 에탄올로 추출한 다음 헥산, 에틸아세테이트, 부탄올, 물 순으로 순차적으로 분획하였다. 분획물 중 에틸아세테이트 분획물은 B16F10 멜라노마 세포 내에서 멜라닌 생성을 억제 효능과 티로시나아제 활성이 우수하였고, 멜라닌 생성에 관련된 단백질 발현 양상을 측정하기 위해 western blotting을 한 결과, 감꼭지 에틸아세테이트 분획물은 농도 의존적으로 무첨가군에 비해 티로시나아제, TRP-1, TRP-의 발현이 억제되었다. 이상의 결과에 따라 감꼭지 에틸아세테이트 분획물의 미백 소재로서 개발할 수 있을 것이라 사료된다.

This study was performed to determine the whitening effect of several solvent fractions of Diospyros kaki extracts. Fractions from ethanol extracts of D. kaki were prepared by a systematic fractionation procedure using hexane, ethyl acetate, butanol, and $H_2O$. The ethyl acetate fractions were used to evaluate the inhibitory effects on tyrosinase activity and melanin synthesis in B16F10 melanoma cells. Ethyl acetate fractions suppressed the expression of tyrosinase, TRP-1 and TRP-2 in B16F10 melanoma cells. These results showed that ethyl acetate fractions of D. kaki could be developed as a skin whitening material in cosmetics.

키워드

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