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버섯복합물의 항산화 효과

Studies on Antioxidant Effect of Mushroom Complex

  • 전동하 (대구한의대학교 화장품약리학과) ;
  • 김희영 (대구한의대학교 화장품약리학과) ;
  • 한상익 (국립식량과학원) ;
  • 김영 (메디웨이코리아) ;
  • 김세기 (이지함화장품) ;
  • 이진태 (대구한의대학교 화장품약리학과)
  • Jun, Dong Ha (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Hui Yeoug (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Han, Sang Ik (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Kim, Young Hun (Mediway Korea Co., Ltd. Skin Science R&D Center) ;
  • Kim, Se Gie (Leejiham Cosmetics Co. Ltd.) ;
  • Lee, Jin Tae (Department of Cosmeceutical Science, Daegu Haany University)
  • 투고 : 2012.12.10
  • 심사 : 2013.03.19
  • 발행 : 2013.03.30

초록

이 연구에서는 동충하초, 영지, 상황 버섯 복합물의 항산화 효과를 평가하였다. 3가지 버섯을 각각 70% 에탄올 추출하여 80:10:10의 비율로 혼합하여 평가를 실시하였다. DPPH radical을 이용한 전자공여능 측정에서는 버섯 복합물이 500 ${\mu}g/ml$의 농도에서 66%의 효과를 나타내었고, ABTS radical cation decolorization 측정은 50 ${\mu}g/ml$에서 73%, 500 ${\mu}g/ml$에서 대조군인 BHA와 유사한 효과를 나타내었다. Superoxide anion radical 소거능 측정은 버섯 복합물 10 ${\mu}g/ml$에서 41%로 대조군인 BHA에 비해 3배 이상의 높은 효과가 있었고, 50 ${\mu}g/ml$, 100 ${\mu}g/ml$에서도 BHA 보다 효과가 높았으나, 500 ${\mu}g/ml$에서는 낮은 효과를 나타내었다. Lipid oxidation 측정결과 $Cu^{2+}$군보다 $Fe^{2+}$군이 지방 산패 억제능이 뛰어났다. SOD 유사활성능 측정은 500 ${\mu}g/ml$에서 13%의 낮은 효과를 나타내었다. 이 결과, 버섯 복합물의 항산화 효과가 아주 우수했으므로, 항산화 소재로서의 가능성을 확인할 수 있었다.

In this study, we evaluated the antioxidant effects of Cordyceps, Ganoderma lucidum (Curtis) P. Karst and Phellinus linteus complexes. Three mushrooms were extracted using 70% ethanol, respectively, in ratios of 80:10:10(?). The electron-donating ability was measured using the DPPH radical mushroom complexes and showed effects of 66% in 500 ${\mu}g/ml$. ABTS radical cation decolorization measured 73% in 50 ${\mu}g/ml$ and had an effect similar to BHA in 500 ${\mu}g/ml$. In their superoxide anion radical scavenging ability, the mushroom complexes showed an effect of 41% in 10 ${\mu}g/ml$. The effect on the control of BHA was more than three times as high as BHA in 50 ${\mu}g/ml$ and 100 ${\mu}g/ml$ concentration. The results showed that lipid oxidation is an excellent inhibitor of lipid oxidation $Cu^{2+}$, compared to the $Fe^{2+}$ group. The results of superoxide dismutase (SOD) activity in the mushroom complexes showed a low effect of 13% in 500 ${\mu}g/ml$. Thus, the results of this study showed that the antioxidant effect of the mushroom complexes was excellent, indicating their potential as an antioxidant material.

키워드

참고문헌

  1. An, C. S., Choi, S. Y., Kim, H. L., Jeon, Y. H., Hur, S. J., Kim, I. H., Park, G. D., Jeoung, Y. J. and Lim, B. O. 2009. Immunomodulatory effects of Phellinus linteus extracts on liver damagy induced by carbon tetrachloride in rats. Korean J Med Crop Sci 17, 217-222.
  2. Ancerewicz, J. E., Migliavacca, P. A., Carrupt, B., Testa, F., Bree, R., Zini, J. P., Tillernent, S., Labidalle, D. and Guyot, A. M. 1998. Chauvent-Monges., Crevat, A. and Ridant, A. L. : Structure property relationships of trimetazidine derivatives and model compounds as potential antioxidants. Free Radical Bio Med 25, 113-120. https://doi.org/10.1016/S0891-5849(98)00072-0
  3. Aoshima, H., Tsunoue, H., Koda, H. and Kiso, Y. 2004. Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. J Agr Food Chem 52, 5240-5244. https://doi.org/10.1021/jf049817s
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1200.
  5. Buege, J. A. and Aust, S. D. 1978. Microsomal lipid peroxidation. Med Enzymol 105, 302-310.
  6. Cheung, L. M., Cheung, P. C. K. and Ooi, V. E. C. 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 80, 1-7. https://doi.org/10.1016/S0308-8146(02)00227-3
  7. Cojocaru, I. M., Cojocaru, M., Musuroi, C., Botezat, M., Lazar, L. and Druta, A. 2004. Lipid peroxidation and catalase in diabetes mellitus with and without ischemic stroke. Rom J Intern Med 42, 423-429.
  8. Comporti, M. 1993. Lipid peroxidation : An overview. pp. 65-79. In: Free Radicals : From Basic Science to Medicine : Molecular and cell biology updates. In Poli, G., Albano, E., and Dianzani, M. U. (eds.), Birkhauser Verlag. Basel, Switzerland.
  9. Jeoung, Y. J., Choi, S. Y., An, C. S., Jeon, Y. H., Park, D. K. and Lim, B. O. 2009. Comparative effect on anti-inflammatory activity of the Phellimus linteus and Phellinus linteus grown in germinated brown rice extracts in murine macrophage RAW 264.7 cells. Korean J Med Crop Sci 17, 97-101.
  10. Kang, J. Y., Kwon, D. K., and Song, Y. J. 2008. The effects of Phellinus linteus extract drink supplementation of metabolic parameters of skeletal muscle and blood in rats. Korean J Exe Nut 12, 53-58.
  11. Kim, H. K., Kim, Y. E., Do, J. R., Lee, Y. C. and Lee, B. Y. 1995. Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J Food Sci Technol 27, 80-85.
  12. Klug, D., Rabani, J. and Fridovich, I. A direct demonstration of the catalytic action of superoxide dismutase through the use of pulse radiolysis. J Biol Chem 247, 4839-4842.
  13. Kobayasi, Y. and Shimizu, D. 1983. Iconography of vegetable wasps and plant worms. Hoikusha Publishing Company Ltd. Osaka.
  14. Lee, B. C., Bae, J. T., Pyo, H. B., Choe, T. B., Kim, S. W., Hwang, H. J. and Yun, J. W. 2003. Biological activities of the polysaccharides produced from submerged culture of the edible Basidimycete Grifola frondosa. Enz Microb Technol 6274, 1-8.
  15. Lie, F., Ooi, V. E. C and Chang, S. T. 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 60, 763-771. https://doi.org/10.1016/S0024-3205(97)00004-0
  16. Marklund, S. and Marklund, G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47, 468.
  17. Martin-Aragon, S., Benedi, J. M. and Villar, A. M. 1997. Modification on antioxidant capacity and lipid peroxidation in mice under fraxetin teatment. J Pharm Pharmacol 49, 49-52.
  18. Mau, J. L., Lin, H. C. and Song, S. F. 2002. Antioxidant properties of several speciality mushroom. Food Res Int 35, 519-526. https://doi.org/10.1016/S0963-9969(01)00150-8
  19. Meneghini, R., Martins, E. A. L. and Calderaro, M. 1993. DNA damage by reactive oxygen species : The role of metal. pp. 102-112, In: Free Radicals : From Basic Science to Medicine : Molecular and cell biology updates. In Poli, G., Albano, E. and Dianzani, M. U. (eds.), Birkhauser Verlag. Basel, Switzerland.
  20. Oh, J. T. and Chung, K. S. 1998. Flowcytometric analysis of the antitumor and immunomodulatory activities of GLB-A and GLB-B, the protein-polysaccaride fractions of the growing tips of Ganoderma Luciderm. Yakhak Hoeji 42, 487-493.
  21. Oogawa, T. 1985. The Chinese medicine dictionary 3, Shogakukan Inc. Tokyo.
  22. Park, S. N. 1997. Sking aging and antioxidant. J Soc Cos Sci Korean 23, 75.
  23. Rhee, Y. K., Han, M. J., Park, S. Y. and Kim, D. H. 2000. In vitro and in vivo antitumor activity of the fruit body of Phellinus linteus. Korean J Food Sci Tech 32, 477-480.
  24. Roberta, R., Nicoletta, P., Anna. P., Ananth, P., Min, Y. and Catherine, R. E. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  25. Saito, M., Sakagami, H. and Fujisawa, S. 2003. Cytotoxicity and apoptosis induction by butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). Anticancer Res 23, 4693-4701.
  26. Shiao, M. S., Lee, K. R., Lin, J. J. and Wang, C. T. 1994. Phytochernicals for Cancer Prevention II. pp. 342, In Ho, C. T. (ed.), Teas, Spices and Herbs. Amreican Society, Washington.
  27. Shin, H. W., Kim, H. W., Choi, E. C., Tho, S. H. and Kim, B. K. 1985. Studies on inorganic composition and immunopotestiating activity of Ganoderma Lucidum in Korea. Korean J Pharmacogn 16, 181-190.
  28. Siddhuraju, P. and Becker, K. 2007. The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) seed extracts. Food Chem 101, 10-19. https://doi.org/10.1016/j.foodchem.2006.01.004
  29. Sung, J. M. 1996. Color Atlas Korea of Cordyceps. Kyohak Publishing Company Ltd. Seoul.
  30. Tsuji, N., Moriwaki, S., Suzuki, Y., Takema, Y. and Imokawa, G. 2001. The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem Photobiol 74, 283-290. https://doi.org/10.1562/0031-8655(2001)074<0283:TROESB>2.0.CO;2
  31. Wiliianm, C. and Waggoner, E. D. 1996. Clinical safety and efficacy testing of cosmetic. Cosmetic Sci Technol Series 8.
  32. Yang, J. H., Lin, H. C. and Mau, J. L. 2002. Antioxidant properties of several commercial mushoom. Food Chem 77, 229-2357. https://doi.org/10.1016/S0308-8146(01)00342-9

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  1. Antioxidative Activity of Mushroom Water Extracts Fermented by Lactic Acid Bacteria vol.43, pp.1, 2014, https://doi.org/10.3746/jkfn.2014.43.1.080
  2. Physiological Properties of Sarcodon aspratus Extracts by Ethanol Concentration vol.43, pp.5, 2014, https://doi.org/10.3746/jkfn.2014.43.5.656