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Studies on Antioxidant Effect of Mushroom Complex

버섯복합물의 항산화 효과

  • Jun, Dong Ha (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Hui Yeoug (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Han, Sang Ik (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Kim, Young Hun (Mediway Korea Co., Ltd. Skin Science R&D Center) ;
  • Kim, Se Gie (Leejiham Cosmetics Co. Ltd.) ;
  • Lee, Jin Tae (Department of Cosmeceutical Science, Daegu Haany University)
  • 전동하 (대구한의대학교 화장품약리학과) ;
  • 김희영 (대구한의대학교 화장품약리학과) ;
  • 한상익 (국립식량과학원) ;
  • 김영 (메디웨이코리아) ;
  • 김세기 (이지함화장품) ;
  • 이진태 (대구한의대학교 화장품약리학과)
  • Received : 2012.12.10
  • Accepted : 2013.03.19
  • Published : 2013.03.30

Abstract

In this study, we evaluated the antioxidant effects of Cordyceps, Ganoderma lucidum (Curtis) P. Karst and Phellinus linteus complexes. Three mushrooms were extracted using 70% ethanol, respectively, in ratios of 80:10:10(?). The electron-donating ability was measured using the DPPH radical mushroom complexes and showed effects of 66% in 500 ${\mu}g/ml$. ABTS radical cation decolorization measured 73% in 50 ${\mu}g/ml$ and had an effect similar to BHA in 500 ${\mu}g/ml$. In their superoxide anion radical scavenging ability, the mushroom complexes showed an effect of 41% in 10 ${\mu}g/ml$. The effect on the control of BHA was more than three times as high as BHA in 50 ${\mu}g/ml$ and 100 ${\mu}g/ml$ concentration. The results showed that lipid oxidation is an excellent inhibitor of lipid oxidation $Cu^{2+}$, compared to the $Fe^{2+}$ group. The results of superoxide dismutase (SOD) activity in the mushroom complexes showed a low effect of 13% in 500 ${\mu}g/ml$. Thus, the results of this study showed that the antioxidant effect of the mushroom complexes was excellent, indicating their potential as an antioxidant material.

이 연구에서는 동충하초, 영지, 상황 버섯 복합물의 항산화 효과를 평가하였다. 3가지 버섯을 각각 70% 에탄올 추출하여 80:10:10의 비율로 혼합하여 평가를 실시하였다. DPPH radical을 이용한 전자공여능 측정에서는 버섯 복합물이 500 ${\mu}g/ml$의 농도에서 66%의 효과를 나타내었고, ABTS radical cation decolorization 측정은 50 ${\mu}g/ml$에서 73%, 500 ${\mu}g/ml$에서 대조군인 BHA와 유사한 효과를 나타내었다. Superoxide anion radical 소거능 측정은 버섯 복합물 10 ${\mu}g/ml$에서 41%로 대조군인 BHA에 비해 3배 이상의 높은 효과가 있었고, 50 ${\mu}g/ml$, 100 ${\mu}g/ml$에서도 BHA 보다 효과가 높았으나, 500 ${\mu}g/ml$에서는 낮은 효과를 나타내었다. Lipid oxidation 측정결과 $Cu^{2+}$군보다 $Fe^{2+}$군이 지방 산패 억제능이 뛰어났다. SOD 유사활성능 측정은 500 ${\mu}g/ml$에서 13%의 낮은 효과를 나타내었다. 이 결과, 버섯 복합물의 항산화 효과가 아주 우수했으므로, 항산화 소재로서의 가능성을 확인할 수 있었다.

Keywords

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