대한본초학회지 (The Korea Journal of Herbology)

  • 제28권2호
  • /
  • Pages.39-44
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  • 2013
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

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GGEx16, GGEx18과 감비통성교낭(減肥通聖膠囊)의 항비만유전자 활성 비교

Comparison among GGEx16, GGEx18 and gambitongseong-capsule for anti-obesity gene activity

  • 오재호 (목원대학교 바이오건강학부) ;
  • 안예지 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소) ;
  • 이혜림 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소) ;
  • 임혜숙 (목원대학교 바이오건강학부) ;
  • 이형희 (목원대학교 바이오건강학부) ;
  • 윤미정 (목원대학교 바이오건강학부) ;
  • 신순식 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소)
  • Oh, Jaeho (Dept. of Life Sciences, Mokwon University) ;
  • Ahn, Ye Ji (Dept. of Formula Science and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University) ;
  • Lee, Hye Rim (Dept. of Formula Science and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University) ;
  • Lim, Hyesook (Dept. of Life Sciences, Mokwon University) ;
  • Lee, Hyunghee (Dept. of Life Sciences, Mokwon University) ;
  • Yoon, Michung (Dept. of Life Sciences, Mokwon University) ;
  • Shin, Soon Shik (Dept. of Formula Science and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University)
  • 투고 : 2013.02.16
  • 심사 : 2013.03.22
  • 발행 : 2013.03.30
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초록

Objectives : Gambigyeongsinhwan 16 (GGEx16), gambigyeongsinhwan 18 (GGEx18) and gambitongseong capsule are shown to be involved in the regulation of obesity. Therefore, the aim of this study was to compare the reporter activity of anti-obesity genes such as peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) and $PPAR{\delta}$ by GGEx16, GGEx18 and gambitongseong capsule. Methods : After NMu2Li liver cells, C2C12 skeletal muscle cells and 3T3-L1 preadipocytes were treated with GGEx16 (1 ${\mu}g/ml$), GGEx18 (1 ${\mu}g/ml$) and different concentrations of gambitongseong capsule, the transactivation of $PPAR{\alpha}$ and $PPAR{\delta}$ was measured by a luciferase reporter gene assay. Results : $PPAR{\alpha}$ reporter gene activity in NMu2Li liver cells and 3T3-L1 preadipocytes was significantly increased by GGEx16, GGEx18 and gambitongseong capsule compared with control, whereas $PPAR{\alpha}$ reporter gene activity in C2C12 skeletal muscle cells was significantly increased by GGEx18 only compared with control. Similarly, $PPAR{\delta}$ reporter gene activity in 3T3-L1 preadipocytes was also significantly increased by GGEx18 compared with control. $PPAR{\delta}$ reporter gene activity in C2C12 skeletal muscle cells was significantly increased by GGEx16 and GGEx18 compared with control although $PPAR{\delta}$ reporter gene activity in NMu2Li liver cells was not changed by these three formulas. Conclusions : These results suggest that all three formulas have the ability to stimulate $PPAR{\alpha}$ and $PPAR{\delta}$ transactivation in animal cell lines with high metabolic rates. In particular, this effects were most prominent in GGEx18-treated cells. In addition, it is likely that GGEx18 may be used as an effective anti-obesity composition.

키워드

참고문헌

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