The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparison among GGEx16, GGEx18 and gambitongseong-capsule for anti-obesity gene activity

GGEx16, GGEx18과 감비통성교낭(減肥通聖膠囊)의 항비만유전자 활성 비교

  • Oh, Jaeho (Dept. of Life Sciences, Mokwon University) ;
  • Ahn, Ye Ji (Dept. of Formula Science and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University) ;
  • Lee, Hye Rim (Dept. of Formula Science and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University) ;
  • Lim, Hyesook (Dept. of Life Sciences, Mokwon University) ;
  • Lee, Hyunghee (Dept. of Life Sciences, Mokwon University) ;
  • Yoon, Michung (Dept. of Life Sciences, Mokwon University) ;
  • Shin, Soon Shik (Dept. of Formula Science and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University)
  • 오재호 (목원대학교 바이오건강학부) ;
  • 안예지 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소) ;
  • 이혜림 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소) ;
  • 임혜숙 (목원대학교 바이오건강학부) ;
  • 이형희 (목원대학교 바이오건강학부) ;
  • 윤미정 (목원대학교 바이오건강학부) ;
  • 신순식 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소)
  • Received : 2013.02.16
  • Accepted : 2013.03.22
  • Published : 2013.03.30
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Abstract

Objectives : Gambigyeongsinhwan 16 (GGEx16), gambigyeongsinhwan 18 (GGEx18) and gambitongseong capsule are shown to be involved in the regulation of obesity. Therefore, the aim of this study was to compare the reporter activity of anti-obesity genes such as peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) and $PPAR{\delta}$ by GGEx16, GGEx18 and gambitongseong capsule. Methods : After NMu2Li liver cells, C2C12 skeletal muscle cells and 3T3-L1 preadipocytes were treated with GGEx16 (1 ${\mu}g/ml$), GGEx18 (1 ${\mu}g/ml$) and different concentrations of gambitongseong capsule, the transactivation of $PPAR{\alpha}$ and $PPAR{\delta}$ was measured by a luciferase reporter gene assay. Results : $PPAR{\alpha}$ reporter gene activity in NMu2Li liver cells and 3T3-L1 preadipocytes was significantly increased by GGEx16, GGEx18 and gambitongseong capsule compared with control, whereas $PPAR{\alpha}$ reporter gene activity in C2C12 skeletal muscle cells was significantly increased by GGEx18 only compared with control. Similarly, $PPAR{\delta}$ reporter gene activity in 3T3-L1 preadipocytes was also significantly increased by GGEx18 compared with control. $PPAR{\delta}$ reporter gene activity in C2C12 skeletal muscle cells was significantly increased by GGEx16 and GGEx18 compared with control although $PPAR{\delta}$ reporter gene activity in NMu2Li liver cells was not changed by these three formulas. Conclusions : These results suggest that all three formulas have the ability to stimulate $PPAR{\alpha}$ and $PPAR{\delta}$ transactivation in animal cell lines with high metabolic rates. In particular, this effects were most prominent in GGEx18-treated cells. In addition, it is likely that GGEx18 may be used as an effective anti-obesity composition.

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References

  1. Yoon KH, Lee HY, Jung YS, Seo BI, Park GR, Yoon MC, Shin SS. Modulation of obesity by Gyeongshingangjeehwan18 in ob/ob mice. Kor J Herbology 2010 ; 25(3) : 1-9.
  2. Lee HY, Yoon KH, Seo BI, Park GR, Yoon MC, Shen ZB, Cui HH, Shin SS. Molecular biologic mechanism of obesity by GGEx18. Kor J Herbology 2011 ; 26(1) : 65-74.
  3. Jung YS, Yoon KH, Choi SB, Yoon MC, Shin SS. Clinical efficacy of Gyeongshingangjeehwan16 according to obeisty related to measurement variables. J Korean Oriental Med Pre. 2008 ; 16(1) : 169-83.
  4. Jung YS, Yoon KH, Choi SB, Yoon MC, Shin SS. Clinical efficacy of Gyeongshingangjeehwan16 according to bioimpedence analysis system. Journal of Oriental Neuropsychiatry 2008 ; 19(1) : 71-81.
  5. Ge Q, Gao ZL, Liu SS. Fat-reducing facebeautifying medicine. CN 001839978 (2006.10.04).
  6. Liu He Jian. hwangjesomunseonmyeongnonbang (Treatise on Explaining Prescriptions from the Emperor's Canon on Medicine) volume 3, first edition. Beijing: China Press of Traditional Chinese Medicine. 2006 : 24-5.
  7. Choi EM, Ryu EK. Effect of Bang-Pung-Tong-Seoung-San on obese patients. J Korean Med Obes Res. 2001 ; 1(1) : 1-11.
  8. Kim BW, Kim DG. Effects of Bangpoongtongsungsankamibang extract on the obese SD rats induced by 2% cholesterol diet. J Korean Oriental Ped. 2007 ; 21(1) : 211-26.
  9. Hwang SJ, Song TW, Oh MS. The Inhibitory Effects of Bangpungtongseoung-san on the Obese gene and Obese Inhibitory about Obese-mouse induced by High Fat Diet. J Korean Oriental Med. 2006 ; 27(1) : 11-22.
  10. Cha MK, Lee JY, Kim DG, Lee KT. Effects of Bangpoongtongsungsan on Adipose Tissue and Hyperlipidemia of 3T3-L1 Induced Rats. J Korean Oriental Ped. 2006 ; 20(2) : 177-96.
  11. Ahn HS, Lee JY, Kim DG. Anti-obestic Effects of Bangpoongtongsungcankamibang(BTSK) in 3T3-L1 preadipocyte cells and the Lipid Metabolism of Obesity induced Rats. J Korean Oriental Ped. 2006 ; 20(1) : 219-40.
  12. Schoonjans K, Staels B, Auwerx J. Role of the peroxisome proliferator-activated receptor (PPAR) in mediating the effects of fibrates and fatty acids on gene expression. J Lipid Res. 1996 ; 37 : 907-25.
  13. Neve BP, Fruchart JC, Staels B. Role of the peroxisome proliferator-activated receptors (PPARs) in atherosclerosis. Biochem Pharmacol. 2000 ; 60 : 1245-50. https://doi.org/10.1016/S0006-2952(00)00430-5
  14. Costet P, Legendre C, More J, Edgar A, Galtier P, Pineau T. Peroxisome proliferator- activated receptor alpha-isoform deficiency leads to progressive dyslipidemia with sexually dimorphic obesity and steatosis. J Biol Chem. 1998 ; 273 : 29577-85. https://doi.org/10.1074/jbc.273.45.29577
  15. Chaput E, Saladin R, Silvestre M, Edgar AD. Fenofibrate and rosiglitazone lower serum triglycerides with opposing effects on body weight. Biochem Biophys Res Commun. 2000 ; 271 : 445-50. https://doi.org/10.1006/bbrc.2000.2647
  16. Muoio DM, MacLean PS, Lang DB, Li S, Houmard JA, Way JM, Winegar DA, Corton JC, Dohm GL, Kraus WE. Fatty acid homeostasis and induction of lipid regulatory genes in skeletal muscles of peroxisome proliferator-activated receptor (PPAR) alpha knock-out mice. Evidence for compensatory regulation by PPAR delta. J Biol Chem. 2002 ; 277(29) : 26089-97. https://doi.org/10.1074/jbc.M203997200
  17. Holst D, Luquet S, Nogueira V, Kristiansen K, Leverve X, Grimaldi PA. Nutritional regulation and role of peroxisome proliferator-activated receptor delta in fatty acid catabolism in skeletal muscle. Biochim Biophys Acta. 2003 ; 1633(1) : 43-50. https://doi.org/10.1016/S1388-1981(03)00071-4
  18. Tanaka T, Yamamoto J, Iwasaki S, Asaba H, Hamura H, Ikeda Y, Watanabe M, Magoori K, Ioka RX, Tachibana K, Watanabe Y, Uchiyama Y, Sumi K, Iguchi H, Ito S, Doi T, Hamakubo T, Naito M, Auwerx J, Yanagisawa M, Kodama T, Sakai J. Activation of peroxisome proliferator-activated receptor delta induces fatty acid beta-oxidation in skeletal muscle and attenuates metabolic syndrome. Proc Nati Acad Sci U S A. 2003 ; 100(26) : 15924-9. https://doi.org/10.1073/pnas.0306981100
  19. Wang YX, Lee CH, Tiep S, Yu RT, Ham J, Kang H, Evans RM. Peroxisome proliferator- activated receptor delta activates fat metabolism to prevent obesity. Cell. 2003 ; 113(2) : 159-70. https://doi.org/10.1016/S0092-8674(03)00269-1