Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Growth Promotion of Tomato Plant under Drought Conditions by Treatment of Rhizobacteria Producing ACC Deaminase and Phytohormones

ACC Deaminase와 식물호르몬 생성 세균 처리에 의한 토마토 식물의 가뭄 조건에서의 생장

  • Seo, Mi-So (Department of Biological Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
  • 서미소 (강원대학교 자연과학대학 생명과학과) ;
  • 송홍규 (강원대학교 자연과학대학 생명과학과)
  • Received : 2013.01.23
  • Accepted : 2013.03.19
  • Published : 2013.03.31
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Abstract

Some rhizobacteria producing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase can make plant to continue growth under the stress conditions through lowering the level of phytohormone, ethylene which inhibits the plant growth and accelerates plant aging. In this study, some rhizobacteria producing ACC deaminase have been isolated from the rhizosphere of plants grown at sand beaches, and identified as Escherichia hermannii m-2, Enterobacter asburiae m-4, Pseudomonas thivervalensis BD2-26 and Pseudomonas brassicacearum subsp. neoaurantiaca BD3-35 through sequencing of 16S rRNA genes. Strain BD3-35 showed the highest activity of ACC deaminase among the isolates, 20.26 ${\alpha}$-ketobutyrate ${\mu}M/mg$ protein/h. Strains BD3-35 and BD2-26 secreted a phytohormone cytokinin, and strains m-4 and m-2 could produce auxin and abscisic acid, respectively. When these bacteria were applied to the 7-day old tomato plant under drought stress for 7 days, strains BD3-35, m-2, and m-4 increased the length of tomato root by 14, 15, and 35%, respectively, and strains m-2, BD2-26 and BD3-35 increased the dry weight of tomato plant by 22, 33, and 68%, respectively compared to the uninoculated control tomatoes. Therefore, these rhizobacteria may be utilized as a microbial fertilizer for the plants under drought stress.

일부 근권세균은 ACC deaminase를 생성하여 식물의 생장을 저해하고 노화를 촉진시키는 식물호르몬 에틸렌의 수준을 낮춤으로써 스트레스 조건 하의 식물의 생장을 지속시킨다. 본 연구에서는 모래사장에서 자라는 식물의 근권에서 ACC deaminase를 생성하는 세균 균주들을 분리하여 16S rDNA 염기서열 분석을 통해 Escherichia hermannii m-2, Enterobacter asburiaem-4, Pseudomonas thivervalensis BD2-26, and Pseudomonas brassicacearum subsp. neoaurantiaca BD3-35로 동정하였다. BD3-35 균주는 이들 중 가장 높은 ACC deaminase 활성, 20.26 ${\alpha}$-ketobutyrate ${\mu}M/mg$ protein/h을 나타내었다. 균주 BD3-35와 BD2-26는 식물호르몬 시토키닌, m-4는 옥신 IAA와 IBA, 그리고 균주 m-2는 ABA 생성능을 가졌다. 이 균주들은 모두 토마토종자 발아 시 유묘의 뿌리신장을 유의성 있게 촉진하였다. 또한 7일간 자란 토마토 식물에 처리하고 가뭄 스트레스 하에서 7일간 재배하였을 때 비접종 대조군에 비해 균주 BD3-35, m-2와 m-4는 토마토 뿌리의 길이를 각각 14, 15와 35% 증가시켰으며, m-2, BD2-26와 BD3-35는 토마토 식물의 건조중량을 각각 22, 33과 68% 증가시켰다. 따라서 이 균주들은 가뭄 스트레스 하의식물을 위한 미생물 비료로 사용될 수 있는 가능성을 보였다.

Keywords

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