Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Protective Effects of Houttuynia cordata Thunb. on Gentamicin-induced Oxidative Stress and Nephrotoxicity in Rats

  • Kang, Changgeun (Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hyungkyoung (Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Hah, Do-Yun (Gyeongnam Livestock promotion Research Institute) ;
  • Heo, Jung Ho (Gyeongnam Livestock promotion Research Institute) ;
  • Kim, Chung Hui (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Kim, Euikyung (Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Jong Shu (Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2013.01.29
  • Accepted : 2013.03.20
  • Published : 2013.03.31
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Abstract

Development of a therapy providing protection from, or reversing gentamicin-sulfate (GS)-induced oxidative stress and nephrotoxicity would be of great clinical significance. The present study was designed to investigate the protective effects of Houttuynia cordata Thunb. (HC) against gentamicin sulfate-induced renal damage in rats. Twenty-eight Sprague-Dawley rats were divided into 4 equal groups as follows: group 1, control; group 2, GS 100 mg/kg/d, intraperitoneal (i.p.) injection; group 3, GS 100 mg/kg/d, i.p. + HC 500 mg/kg/d, oral; and group 4, GS 100 mg/kg/d i.p. + HC 1000 mg/kg/d, oral administration). Treatments were administered once daily for 12 d. After 12 d, biochemical and histopathological analyses were conducted to evaluate oxidative stress and renal nephrotoxicity. Serum levels of creatinine, malondialdehyde (MDA), and blood urea nitrogen (BUN), together with renal levels of MDA, glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were quantified to evaluate antioxidant activity. Animals treated with GS alone showed a significant increase in serum levels of creatinine, BUN, and MDA, with decreased renal levels of GSH, SOD, and CAT. Treatment of rats with HC showed significant improvement in renal function, presumably as a result of decreased biochemical indices and oxidative stress parameters associated with GS-induced nephrotoxicity. Histopathological examination of the rat kidneys confirmed these observations. Therefore, the novel natural antioxidant HC may protect against GSinduced nephrotoxicity and oxidative stress in rats.

Keywords

References

  1. Khan, S.A., Priyamvada, S., Faroog, N., Khan, S., Khan, M.W. and Yusufi, A.N. (2009) Protective effect of green tea extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney. Pharmacol. Res., 59, 254-262. https://doi.org/10.1016/j.phrs.2008.12.009
  2. Salgado, C.M., Hernades, F.L. and Novoa, J.M. (2007) Glomerular nephrotoxicity of amino nucleosides. Toxicol. Appl. Pharmacol., 223, 86-98. https://doi.org/10.1016/j.taap.2007.05.004
  3. Choi, J.J., Moffett, B.S., McDade, E.J. and Palazzi, D.L. (2011) Altered gentamicin serum concentrations in obese pediatric patients. Pediatr. Infect. Dis. J., 30, 347-349. https://doi.org/10.1097/INF.0b013e3181ff023e
  4. Balakumar, P., Rohilla, A. and Thangathirupathi, A. (2010) Gentamicin-inducied nephrotoxicity : Do we have promising therapeutic approach to blunt it? Pharmacol. Res., 62, 179-186. https://doi.org/10.1016/j.phrs.2010.04.004
  5. Ali, B.H. (2003) Agents ameliorating or augmenting experimental gentamicin nephrotoxicity: Some recent research. Food Chem. Toxicol., 41, 1447-1452. https://doi.org/10.1016/S0278-6915(03)00186-8
  6. Soliman, K.M., Abdul-Hamid, M. and Othman, A.I. (2007) Effect of carnosine on gentamicin induced nephrotoxicity. Med. Sci. Monit., 13, 73-83.
  7. Romero, F., Pérez, M., Chávez, M., Parra, G. and Durante, P. (2009) Effect of uric acid on gentamicin- induced nephrotoxicity in rats role of matrix metalloproteinases 2 and 9. Basic Clin. Pharmacol. Toxicol., 105, 416-424. https://doi.org/10.1111/j.1742-7843.2009.00466.x
  8. Abdel-Naim, A.B., Abdel-wahab, M.H. and Attia, F.F. (1999) Protective effects of vitamin E and probucol against gentamicin- induced nephrotoxicity in rats. Pharmacol. Res., 40, 183-187. https://doi.org/10.1006/phrs.1999.0494
  9. Bledsoe, G., Crickman, S., Mao, J., Xia, C.F., Murakami, H., Chao, L. and Chao, J. (2006) Kallikrein/kinin protects against gentamicin-induced nephrotoxicity by inhibition of inflammation and apoptosis. Nephrol. Dial. Transplant., 21, 624-633. https://doi.org/10.1093/ndt/gfi225
  10. Lakshmi, B.V.S. and Sudhakar, M. (2010) Protective effect of Zingiber officinale on gentamicin induced nephrotoxicity in rats. Int. J. Pharmacol., 6, 58-62. https://doi.org/10.3923/ijp.2010.58.62
  11. Ali, B.H., Al-Salam, S., Al-Husseini, I. and Nemmar, A. (2009) Comparative protective effect of N-acetylcysteine and tetramethylpyrazine in rats with gentamicin nephrotoxicity. J. Appl. Toxicol., 29, 302-307. https://doi.org/10.1002/jat.1409
  12. Koyner, J.L., Sher Ali, R. and Murray, P.T. (2008) Antioxidants. Do they have a place in the prevention or therapy of acute kidney injury? Nephron Exp. Nephrol., 109, 109-117. https://doi.org/10.1159/000142935
  13. Baliga, R., Ueda, N., Walker, P.D. and Shah, S.V. (1999) Oxidant mechanisms in toxic acute renal failure. Drug Metab. Rev., 31, 971-997. https://doi.org/10.1081/DMR-100101947
  14. Kalayarasan, S., Prabhu, P.N., Sriram, N., Manikandan, R., Arumugam, M. and Sudhandiran, G. (2009) Diallyl sulfide enhances antioxidants and inhibits inflammation through the activation of Nrf2 against gentamicin-induced nephrotoxicity in Wistar rats. Eur. J. Pharmacol., 606, 162-171. https://doi.org/10.1016/j.ejphar.2008.12.055
  15. Yaman, I. and Balikci, E. (2010) Protective effect of Nigella sativa against gentamicin-induced nephrotoxcity in rats. Exp. Toxicol. Pathol., 62, 183-190. https://doi.org/10.1016/j.etp.2009.03.006
  16. Walker, P.D., Barri, Y. and Shah, S.V. (1999) Oxidant mechanisms in gentamiain nephrotoxicity. Renal Faillure, 21, 433-442. https://doi.org/10.3109/08860229909085109
  17. Karadeniz, A., Yildirim, A., Simsek, N., Kalkan, Y. and Celebi, F. (2008) Spirulina platensis protect against gentamicin- induced nephrotoxicity in rats. Phytother. Res., 22, 1506-1510. https://doi.org/10.1002/ptr.2522
  18. Thounaojam, M.C., Jadeja, R.N., Devkar, R.V. and Ramachandran, A.V. (2010) Siad rhomboidea. Roxb leaf extract ameliorates gentamicin induced nephrotoxicity and renal dysfunction in rats. J. Ethnopharmacol., 132, 365-367. https://doi.org/10.1016/j.jep.2010.08.037
  19. Raju, S., Kavimani, S., Maheshwara Rao, V.U., Reddy, K.S. and Kumar, G.V. (2011) Floral extract of Tecoma stans: A potent inhibitor of gentamicin-induced nephrotoxicity in vivo. Asian Pac. J. Trop. Med., 4, 680-685. https://doi.org/10.1016/S1995-7645(11)60173-9
  20. Tavafi, M., Ahmadvand, H. and Toolabi, P. (2012) Inhibitory effect of Olive leaf extract on gentamicin-induced nephrotoxicity in rats. Iran. J. Kidney Dis., 6, 25-32.
  21. Lai, K.C., Chiu, Y.J. Tang, Y.J., Lin, K.L., Chiang, J.H., Jiang, Y.L., Jen, H.F., Kuo, Y.H., Agamaya, S., Chung, J.G. and Yang, J.S. (2010) Houttuynia cordata Thunb extract inhibits cell growth and induces apoptosis in human primary colorectal cancer cells. Anticancer Res., 30, 3549-3556.
  22. Chen, Y.Y., Liu, J.F., Chen, C.M., Chao, P.Y. and Chang, T.J. (2003) A study of the antioxidative and antimutagenic effects of Houttuynia cordata Thunb. using an oxidized frying oil-fed model. J. Nutr. Sci. Vitaminol., 49, 327-333. https://doi.org/10.3177/jnsv.49.327
  23. Hah, D.S, Kim, C.H., Kim, G.S., Kim, E.G. and Kim, J.S. (2005) Antioxidative effects of traditional medicinal plants on lipd peroxidation. Korean J. Vet. Res., 45, 341-350.
  24. Hah, D.S., Kim, C.H., Ryu, J.D., Kim, E.K. and Kim, J.S. (2007) Evaluation of protective effects of Houttuynia cordata on $H_{2}O_{2}$-induced oxidative DNA damage using an alkaline Comet assay in Human HepG2 cells. J. Toxicol. Public Health, 23, 25-31.
  25. Hah, D.S., Kim, C.H., Kim, E.K., Kang, C.B. and Kim, J.S. (2007) Antioxidative effects of Houttuynia cordata Thunb. on non-lipid oxidative damage. Korean J. Vet. Res., 47, 25-32.
  26. Miyata, M., Koyama, T. and Yazawa, K. (2010) Water extract of Houttuynia cordata Thunb. leveas exert anti-obesiy effects by inhibiting fatty acid and glycerol absorption. J. Nutr. Sci. Vitaminol., 56, 150-156. https://doi.org/10.3177/jnsv.56.150
  27. Hayashi, K., Kamiya, M. and Hayashi, T. (1995) Virucial effects of the steam distillate from Houttuynia cordata and its components on HV-1, influenza virus, and HIV. Plant. Med., 61, 237-241. https://doi.org/10.1055/s-2006-958063
  28. Chang, J.S., Chiang, L.C., Chen, C.C., Liu, L.T., Wang, K.C. and Lin, C.C. (2001) Antileukemic activity of Bidens pilosal. var. minor (Blume) Sherff and Houttuynia cordata Thunb. Am. J. Chin. Med., 29, 303-312. https://doi.org/10.1142/S0192415X01000320
  29. Ren, X., Sui, X. and Yin, J. (2011) The effect of Houttuynia cordata injection on pseudorabies herpesvirus (PrV) infection in vitro. Pharm. Biol., 49, 161-166. https://doi.org/10.3109/13880209.2010.505242
  30. Liang, M., Qi, M., Zhang, C., Zhou, S., Fu, R. and Huang, J. (2005) Gas chromatography-mass spectrometry analysis of volatile compounds from Houttuynia cordata Thunb after extraction by solid-phase microextraction, flash evaporation and steam distillation. Anal. Chem. Acta, 531, 97-104. https://doi.org/10.1016/j.aca.2004.09.082
  31. Lu, H., Wu, X., Liang, Y. and Zhang, J. (2006) Variation in chemical composition and antibacterial activities of essential oils from two species of Houttuynia THUNB. Chem. Pharm. Bull., 54, 936-940. https://doi.org/10.1248/cpb.54.936
  32. Ch, M.I., Wen, Y.F. and Cheng, Y. (2007) Gas chromatographic/ Mass spectrometric analysis of the essential oil of Houttuynia cordata Thunb by using on-column methylation with tetramethylammonium acetate. J. AOAC Int., 90, 60-67.
  33. KFDA. (2005) Testing Guidelines for Safety Evaluation of Drugs. KFDA, 60, issued by the KFDA.
  34. Karahan, I., Atessahin, A., Yilmaz, S., Cerbisat, A.O. and Sakin, F. (2005) Protectiver effect of lycopene-induced oxidative stress and nephrotoxicity in rats. Toxicol., 215, 198-204. https://doi.org/10.1016/j.tox.2005.07.007
  35. Tavafi, M., Ahmadvand, H. and Toolabi, P. (2012) Inhibitory effect of Olive leaf extract on gentamicin-induced nephrotoxicity in rats. Iran. J. Kidney Dis., 6, 25-32.
  36. Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with Folin phenol reagent. J. Biol. Chem., 193, 265-275.
  37. Tietze, F. (1969) Enzymic method for quantitative determination of nanogram amounts to total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Biochem., 27, 502-522. https://doi.org/10.1016/0003-2697(69)90064-5
  38. Anderson, M.E. (1985) Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol., 113, 548-555. https://doi.org/10.1016/S0076-6879(85)13073-9
  39. Sun, Y., Oberley, L.W. and Li, Y. (1988) A simple method for clinical assay of superoxide dismutase. Clin. Chem., 34, 497-500.
  40. Mihara, M. and Uchiyama, M. (1978) Detremination of malondialdehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem., 86, 271-278. https://doi.org/10.1016/0003-2697(78)90342-1
  41. Maehly, A.C. and Chance. B. (1954) The assay of catalases and peroxidases. Methods Biochem. Anal., 1, 357-424.
  42. Aebi, H. (1984) Catalase in vitro. Methods Enzymol., 105, 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3
  43. Hussain, T., Gupta, R.K., Sweey, K., Eswaran, B., Vijayakumar, M. and Rao, C.V. (2012) Nephroprotective activity of Solanum xanthocarpum fruit extract against gentamicininduced nephrotoxicity and renal function in experimental rodents. Asian Pac. J. Trop. Med., 5, 686-691. https://doi.org/10.1016/S1995-7645(12)60107-2
  44. Mazzon, E., Britti, D., De Satrro, A., Caputi, A.P. and Cuzzocrea, S. (2001) Effect of N-acetylcysteine on gentamicinmediated nephropathy in rats. Eur. J. Pharmacol., 424, 75-83. https://doi.org/10.1016/S0014-2999(01)01130-X
  45. Randielovic, P., Veljkovic, S., Stojiljkovic, N., Jankovic- Velickovic, L., Sokolovic, D., Stoiljkovic, M. and Ilic, I. (2012) Salicylic acid attenuates gentamicin-induced nephrotoxicity in rats. Sci. World J., 6, 1-6.
  46. Gilbert, D.N., Wood, C.A., Kohlepp, S.J., Houghton, D.C., Finkbeiner, H.C., Lindsley, J. and Bennett, W.M. (1989) Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity. J. Infect. Dis., 159, 945-953. https://doi.org/10.1093/infdis/159.5.945
  47. Farombi, E.O. and Ekor, M. (2006) Curcumin attenuates gentamicin- induced renal oxidative damage in rats. Food Chem. Toxicol., 44, 1443-1448. https://doi.org/10.1016/j.fct.2006.05.005
  48. Cuzzocrea, S., Mazzon, E., Dugo, L., Serraino, I., Di Paola, R., Britti, D., De Sarro, A., Pierpaoili, S., Caputi, A., Masini, E. and Salvemini, D. (2002) A role for superoxide in gentamicin- mediated nephropathy in rats. Eur. J. Pharmacol., 450, 67-76. https://doi.org/10.1016/S0014-2999(02)01749-1

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