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Fermentation of Pentose and Hexose Derived from Cellulosic Food Wastes by Mixed Yeast

공기 주입 방법에 따른 셀룰로오스계 음식물류 폐기물 유래의 오탄당과 육탄당의 동시발효

  • Jeong, Seung-Mi (Department of Environmental Engineering & Biotechnology, Mokpo National Maritime University) ;
  • Kim, Yong-Jin (Department of Environmental Engineering & Biotechnology, Mokpo National Maritime University)
  • 정승미 (목포해양대학교 환경.생명공학과) ;
  • 김용진 (목포해양대학교 환경.생명공학과)
  • Received : 2013.01.10
  • Accepted : 2013.01.17
  • Published : 2013.03.25

Abstract

It is indispensable to increase the conversion rate of a reducing sugars such as pentose and hexose derived from cellulosic wastes for a highly efficient bioethanol fermentation from food wastes. The saccharification liquid from cellulosic substrates such as vegetable food wastes contained lots of hexose like glucose and pentose like xylose. Since Saccharomyces-based yeasts could not convert xylose to bioethanol, Pichia stipitis which could directly ferment xylose to ethanol was chosen. After selecting Saccharomyces coreanus and P. stipitis, fermentation characteristics by mixture of two yeasts were investigated. As a result, it was verified the production of ethanol was enhanced by the co-fermentation, although there were somewhat differences between the fermentation characteristics by the aeration methods. Moreover, the consumption of pentose, hexose and disaccharide was obviously observed, and aeration in the process of fermentation seemed to stimulate the activity of P. stipitis.

Keywords

References

  1. 정인환, 2012, "더러운 에너지, 바이오연료", 한겨레21, 2012. 02.13 제897호.
  2. Searchinger, T., Heimlich, R., Houghton, R. A., Dong, F., Elobeid, A., Fabiosa, J., Tokgoz, S., Hayes, D., Yu, t. H., 2008, "Use of U.S. Croplands for Biofuels Increases Greenhouse Gases Through Emissions from Land-Use Change", Science, Vol. 319, No. 5867, pp. 1238-1240. https://doi.org/10.1126/science.1151861
  3. 환경부, 2007, "제3차(2006-2007년) 전국 폐기물 통계조사".
  4. Jeffries, T. W., 1983, "Utilization of xylose by bacteria, Yeasts and fungi", Advances in Biochemical Engineering Biotechnology, Vol. 27, pp. 1-32.
  5. Slininger, P. J., Bothast, R. J., Okos, M. R., Ladish, M. R., 1985, "Comparative evaluation of ethanol production by xylose fermenting yeasts presented high xylose concentration", Biotechnol. Lett., Vol. 7, pp. 431-436. https://doi.org/10.1007/BF01166218
  6. Dellweg, H., Rizzi, M., Methner, H., Debus, D., 1984, "Xylose fermentation by yeasts, 3. Composition of Pachysolen tannophilus and Pichia stipitis", Biotechnol. Lett., Vol. 6, pp. 395-400. https://doi.org/10.1007/BF00138012
  7. du Preez, J. C., van der Walt, J. P., 1983, "Fermentation of D-xylose to ethanol by a strain of Candida tropicalis", Biotechnol. Lett., Vol. 5, pp. 357-362. https://doi.org/10.1007/BF01141138
  8. Schneider, H., Wang, P. Y., Chan, Y. K., Maleszka, R., 1981, "Conversion of D-xylose into ethanol by the yeast Pachysolen tannophilus", Biotechnol. Lett., Vol. 3, pp. 89-92. https://doi.org/10.1007/BF00145116
  9. Slilinger, P. J., Bothast, R. J., Van Cauwenberge J. E., Kurtzman, C. P., 1982, "Conversion of D-xylose to ethanol by the Pachysolen tannophilus", Biotechnol. Bioeng., Vol. 24, pp. 371-384. https://doi.org/10.1002/bit.260240210
  10. Laplace, J. M., Delgenes, J. P., Moletta, R., Navarro, J. M., 1991, "Alcoholic fermentation of glucose and xylose by Pichia stipitis, Candida shehatae, Sacchromyces cerevisiae and Zymomonas mobils: oxygen requirement as a key factor", Applied Microbiology Biotechnology, Vol. 36, pp. 158-162. https://doi.org/10.1007/BF00164412
  11. 진길선, 2011, "바이오에탄올 생산을 위한 섬유소계 바이오매스의 고효율 당화", 목포해양대학교 석사학위논문.
  12. 유대식, 2003, "Saccharomyces coreanus IFO 1833의 생리학적 특성", 기초과학연구논집 (계명대학교), Vol. 22, pp. 47-50.
  13. Slininger, P. J., Bothast, R. J., Ladisch, M. R., Okos, M. R., 1990, "Optimum pH and temperature conditions for xylose fermentation by Pichia stipitis", Biotechnology and Bioengineering, Vol. 35, pp. 727-731. https://doi.org/10.1002/bit.260350710
  14. Jeong, S.-M., Kim, Y.-J., Lee, D.-H., 2012, "Ethanol production by co-fermentation of hexose and pentose from food wastes using Saccharomyces coreanus and Pichia stipitis", Korean Journal of Chemical Engineering, Vol. 29, No. 8, pp. 1038-1043. https://doi.org/10.1007/s11814-011-0282-3
  15. Miller, G. L., 1959, "Use of dinitrosalicylic acid reagent for determination of reducing sugar", Analytical Chemistry, Vol. 31, pp. 426-428. https://doi.org/10.1021/ac60147a030
  16. 아산제약, 2012, "글루코오스 분석용 킷 사용설명서", http://www.asanpharm.co.kr/(2012.11.26.현재).
  17. 농촌진흥청, 2010, "농식품 종합정보 시스템 > 식품영양 기능성 정보 > PDF 파일 다운로드 > 식품성분표 제7개정판 1권.
  18. 정승미, 2011, "Saccharomyces coreanus와 Pichia stipitis를 이용한 음식물류 폐기물 기원 육탄당 및 오탄당의 에탄올 발효", 서울시립대학교 공학박사학위논문.

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