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Comparison of Antioxidant Activities by Different Extraction Temperatures of Some Commercially Available Cultivars of Rice Bran in Korea

추출온도에 따른 국내 벼 품종 미강의 항산화 활성 비교

  • Lee, Jeong-Heui (National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Sea-Kwan (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Dae-Jung (National Institute of Crop Science, Rural Development Administration) ;
  • Yoon, Mi-Ra (National Institute of Crop Science, Rural Development Administration) ;
  • Chun, Areum (National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Im-Soo (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jeom-Sig (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Yeon-Gyu (National Institute of Crop Science, Rural Development Administration)
  • 이정희 (농촌진흥청 국립식량과학원) ;
  • 오세관 (농촌진흥청 국립식량과학원) ;
  • 김대중 (농촌진흥청 국립식량과학원) ;
  • 윤미라 (농촌진흥청 국립식량과학원) ;
  • 천아름 (농촌진흥청 국립식량과학원) ;
  • 최임수 (농촌진흥청 국립식량과학원) ;
  • 이점식 (농촌진흥청 국립식량과학원) ;
  • 김연규 (농촌진흥청 국립식량과학원)
  • Received : 2012.11.29
  • Accepted : 2013.01.16
  • Published : 2013.03.31

Abstract

This study was conducted to determine antioxidant compounds and antioxidant activities of rice bran at temperatures of $10^{\circ}C$, $25^{\circ}C$, and $60^{\circ}C$ using 70% ethanol solution. DPPH, ABTS radical scavenging activities and reducing power have been used to investigate the relative antioxidant activities of extracts. The contents of total polyphenol and total flavonoids in the 70% ethanol extracts were measured by spectrophotometer. The total polyphenol content in the rice bran extracts were 790.39~930.07, 782.71~1,039.57, and 848.22~1,052.96 mg gallic acid eq./100 g in $10^{\circ}C$, $25^{\circ}C$ and $60^{\circ}C$, respectively. Antioxidant activities, such as DPPH, ABTS radical scavenging activity and reducing power, in all cultivars and temperatures was the highest in Cheonghaejinmi rice bran extracted at $25^{\circ}C$. The results obtained in the in vitro models suggest that extracts from rice bran, especially Cheonghaejinmi, has significant health-promoting effects, as it exhibits antioxidant activities.

본 연구에서는 몇 가지 벼 품종(삼광, 미광, 칠보, 청해진미 및 조운)의 도정 부산물인 미강에 대한 추출 온도에 따른 70% 에탄올 추출물의 항산화 성분(polyphenol, flavonoid)과 항산화 활성(DPPH radical 소거능, ABTS radical 소거능, 환원력)을 비교 분석하여 기능적 가치를 평가함으로써 이용 가능성을 살펴보고자 하였다. 추출 온도에 따른 미강의 항산화 성분을 측정한 결과, 총 polyphenol 함량에서는 추출 온도가 높을수록 다소 증가하는 경향을 나타낸 반면, 총 flavonoid 함량은 추출 온도에 따른 차이는 보이지 않았다. 항산화 활성 측정 결과, DPPH radical 소거능을 제외한 총 항산화력(ABTS radical 소거능)과 환원력은 온도가 높아짐에 따라 유의적으로 증가하는 경향을 나타내었다. 또한 조사되어진 품종들의 미강 최적 추출조건은 청해진미로 $25^{\circ}C$에서 추출하였을 경우에 가장 우수한 항산화 효과를 얻을 수가 있었다. 이에 본 연구결과를 토대로 최적의 기능성 성분을 활용할 수 있는 추출 온도를 제공함으로써 도정 부산물의 최적 추출조건 및 품종별 쌀의 기초 자료를 제공할 수 있을 것이라 사료된다.

Keywords

References

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