Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Detection of Gnathostoma spinigerum Antibodies in Sera of Non-Traumatic Subarachnoid Hemorrhage Patients in Thailand

  • Kitkhuandee, Amnat (Division of Neurosurgery, Department of Surgery, Faculty of Medicine, Khon Kaen University) ;
  • Munkong, Waranon (Department of Radiology, Faculty of Medicine, Khon Kaen University) ;
  • Sawanyawisuth, Kittisak (Department of Medicine, Faculty of Medicine, Khon Kaen University) ;
  • Janwan, Penchom (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Maleewong, Wanchai (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Intapan, Pewpan M. (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
  • Received : 2013.05.30
  • Accepted : 2013.10.11
  • Published : 2013.12.31
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Abstract

Gnathostoma spinigerum can cause subarachnoid hemorrhage (SAH). The detection of specific antibodies in serum against G. spinigerum antigen is helpful for diagnosis of neurognathostomiasis. There is limited data on the frequency of G. spinigerum infection in non-traumatic SAH. A series of patients diagnosed as non-traumatic SAH at the Srinagarind Hospital, Khon Kaen University, Thailand between January 2011 and January 2013 were studied. CT or MR imaging of the brain was used for diagnosis of SAH. Patients were categorized as aneurysmal subarachnoid hemorrhage (A-SAH) or non-aneurysmal subarachnoid hemorrhage (NA-SAH) according to the results of cerebral angiograms. The presence of specific antibodies in serum against 21- or 24-kDa G. spinigerum antigen was determined using the immunoblot technique. The detection rate of antibodies was compared between the 2 groups. Of the 118 non-traumatic SAH patients for whom cerebral angiogram and immunoblot data were available, 80 (67.8%) patients had A-SAH, whereas 38 (32.2%) had NASAH. Overall, 23.7% were positive for specific antibodies against 21- and /or 24-kDa G. spinigerum antigen. No significant differences were found in the positive rate of specific antibodies against G. spinigerum in both groups (P-value=0.350).

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References

  1. Ingall T, Asplund K, Mahonen M, Bonita R. A multinational comparison of subarachnoid hemorrhage epidemiology in the WHO MONICA stroke study. Stroke 2000; 31: 1054-1061. https://doi.org/10.1161/01.STR.31.5.1054
  2. Ammar A, al-Rajeh S, Ibrahim AW, Chowdhary UM, Awada A. Pattern of subarachnoid haemorrhage in Saudi Arabia. Acta Neurochir (Wien) 1992; 114: 16-19. https://doi.org/10.1007/BF01401108
  3. Uysal E, Yanbuloglu B, Erturk M, Kilinc BM, Basak M. Spiral CT angiography in diagnosis of cerebral aneurysms of cases with acute subarachnoid hemorrhage. Diagn Interv Radiol 2005; 11: 77-82.
  4. Urbach H, Zentner J, Solymosi L. The need for repeat angiography in subarachnoid haemorrhage. Neuroradiology 1998; 40: 6-10. https://doi.org/10.1007/s002340050528
  5. Waikagul J, Diaz Chamacho SP. Gnathostomiasis. In Murrell KD, Fried B eds, Food-borne Parasitic Zoonoses. World Class Parasites, Vol. 11. New York, USA. Springer. 2007, p 235-261. https://doi.org/10.1007/978-0-387-71358-8_7
  6. Nawa Y, Katchanov J, Yoshikawa M, Rojekittikhun W, Dekumyoy P, Kusolusuk T, Dorn Wattanakulpanich D. Ocular gnathostomiasis: a comprehensive review. J Trop Med Parasitol 2010; 33: 77-86.
  7. Katchanov J, Sawanyawisuth K, Chotmongkol V, Nawa Y. Neurognathostomiasis, a neglected parasitosis of the central nervous system. Emerg Infect Dis 2011; 17: 1174-1180. https://doi.org/10.3201/eid1707.101433
  8. Sawanyawisuth K, Chlebicki MP, Pratt E, Kanpittaya J, Intapan PM. Sequential imaging studies of cerebral gnathostomiasis with subdural hemorrhage as its complication. Trans R Soc Trop Med Hyg 2009; 103: 102-104. https://doi.org/10.1016/j.trstmh.2008.09.011
  9. Sangchan A, Sawanyawisuth K, Intapan PM, Mahakkanukrauh A. Outward migration of Gnathostoma spinigerum in interferon-alpha treated hepatitis C patient. Parasitol Int 2006; 55: 31-32. https://doi.org/10.1016/j.parint.2005.09.002
  10. Intapan PM, Khotsri P, Kanpittaya J, Chotmongkol V, Sawanyawisuth K, Maleewong W. Immunoblot diagnostic test for neurognathostomiasis. Am J Trop Med Hyg 2010; 83: 927-929. https://doi.org/10.4269/ajtmh.2010.10-0113
  11. Kitkhuandee A, Thammaroj J, Munkong W, Duangthongpon P, Thanapaisal C. Cerebral angiographic findings in patients with non-traumatic subarachnoid hemorrhage. J Med Assoc Thai 2012; 95(suppl): 23-55 (in press). Full text. e-Journal: http://jmat.mat.or.th.
  12. Sawanyawisuth K, Tiamkao S, Nitinavakarn B, Dekumyoy P, Jitpimolmard S. MR imaging findings in cauda equina gnathostomiasis. AJNR Am J Neuroradiol 2005; 26: 39-42.
  13. Sawanyawisuth K, Tiamkao S, Kanpittaya J, Dekumyoy P, Jitpimolmard S. MR imaging findings in cerebrospinal gnathostomiasis. AJNR Am J Neuroradiol 2004; 25: 446-449.
  14. Kanpittaya J, Sawanyawisuth K, Intapan PM, Khotsri P, Chotmongkol V, Maleewong W. A comparative study of neuroimaging features between human neuro-gnathostomiasis and angiostrongyliasis. Neurol Sci 2012; 33: 893-898. https://doi.org/10.1007/s10072-011-0864-1