Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

A Modified Formalin-Ether Concentration Technique for Diagnosis of Human Strongyloidiasis

  • Anamnart, Witthaya (Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University) ;
  • Intapan, Pewpan M. (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Maleewong, Wanchai (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
  • Received : 2013.05.30
  • Accepted : 2013.10.11
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

We compared the efficacy and applicability of a modified formalin-ether concentration technique (M-FECT) to the conventional FECT (C-FECT) and the agar plate culture (APC) method for the detection of Strongyloides stercoralis larvae. For this purpose, we used 600 human fecal specimens collected in an endemic area of southern Thailand. In the M-FECT, we used 2 layers of wire meshes, instead of gauze, to avoid the loss by absorption/adhesion of larvae to the gauze during filtration, and we reduced the exposure time of S. stercoralis larvae in stool samples to formalin. By such simple modifications, the efficacy of M-FECT has become comparable to APC and was much better than that of C-FECT for the diagnosis of strongyloidiasis.

Keywords

References

  1. Grove DI. Human strongyloidiasis. Adv Parasitol 1996; 38: 251-309. https://doi.org/10.1016/S0065-308X(08)60036-6
  2. Arakaki T, Iwanaga M, Kinjo F, Saito A, Asato R, Ikeshiro T. Efficacy of agar plate culture in detection of Strongyloides stercoralis infection. J Parasitol 1990; 76: 425-428. https://doi.org/10.2307/3282680
  3. Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Nakamura Y, Tani S, Ieda M, Tomita K, Tomita S, Hattan N, Mori M, Makino S. An evaluation of the agar plate method for the detection of Strongyloides stercoralis in northern Thailand. J Trop Med Hyg 1990; 93: 183-188.
  4. Intapan PM, Maleewong W, Wongsaroj T, Singthong S, Morakote N. Comparison of the quantitative formalin ethyl acetate concentration technique and agar plate culture for diagnosis of human strongyloidiasis. J Clin Microbiol 2005; 43: 1932-1933. https://doi.org/10.1128/JCM.43.4.1932-1933.2005
  5. Kaminsky RG. Evaluation of three methods for laboratory diagnosis of Strongyloides stercoralis infection. J Parasitol 1993; 79: 277-280. https://doi.org/10.2307/3283519
  6. Anamnart W, Pattanawongsa A, Intapan PM, Maleewong W. Factors affecting recovery of Strongyloides stercoralis larvae: an approach to a newly modified formalin-ether concentration technique for diagnosis of strongyloidiasis. J Clin Microbiol. 2010; 48: 97-100. https://doi.org/10.1128/JCM.01613-09
  7. Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 1991; 45: 518-521.
  8. Beaver PC, Jung RC, Cupp EW. Examination of specimens for parasites. In Beaver PC, Jung RC, Cupp EW, eds, Clinical Parasitology, 9th ed. Philadelphia, PA. Lea & Febiger. 1984, p 733-758.
  9. Jongwutiwes, S, Charoenkorn M, Sitthicharoenchai P, Akarabovorn P, Putaporntip C. Increased sensitivity of routine laboratory detection of Strongyloides stercoralis and hookworm by agar-plate culture. Trans R Soc Trop Med Hyg 1999; 93: 398-400. https://doi.org/10.1016/S0035-9203(99)90132-3

Cited by

  1. Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand vol.78, pp.12, 2013, https://doi.org/10.1292/jvms.16-0293
  2. Risk factors for Opisthorchis viverrini infection in Nakhon Phanom, Thailand, where the infection is highly endemic vol.12, pp.1, 2013, https://doi.org/10.1515/abm-2018-0030
  3. Strongyloides stercoralis larvae found for the first time in tap water using a novel culture method vol.117, pp.12, 2013, https://doi.org/10.1007/s00436-018-6078-1