Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Ultrastructure of Spermatogenesis in the Testis of Paragonimus heterotremus

  • Uabundit, Nongnut (Department of Anatomy, Faculty of Medicine, Khon Kaen University) ;
  • Kanla, Pipatphong (Department of Anatomy, Faculty of Medicine, Khon Kaen University) ;
  • Puthiwat, Phongphithak (Department of Anatomy, Faculty of Medicine, Khon Kaen University) ;
  • Arunyanart, Channarong (Department of Anatomy, Faculty of Medicine, Khon Kaen University) ;
  • Chaiciwamongkol, Kowit (Department of Anatomy, Faculty of Medicine, Khon Kaen University) ;
  • Maleewong, Wanchai (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Intapan, Pewpan M. (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Iamsaard, Sitthichai (Department of Anatomy, Faculty of Medicine, Khon Kaen University) ;
  • Hipkaeo, Wiphawi (Department of Anatomy, Faculty of Medicine, Khon Kaen University)
  • Received : 2013.04.30
  • Accepted : 2013.10.11
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Lung fluke, Paragonimus heterotremus, is a flatworm causing pulmonary paragonimiasis in cats, dogs, and humans in Southeast Asia. We examined the ultrastructure of the testis of adult P. heterotremus with special attention to spermatogenesis and spermiogenesis using scanning and transmission electron microscopy. The full sequence of spermatogenesis and spermiogenesis, from the capsular basal lamina to the luminal surface, was demonstrated. The sequence comprises spermatogonia, spermatocytes with obvious nuclear synaptonemal complexes, spermatids, and eventual spermatozoa. Moreover, full steps of spermatid differentiation were shown which consisted of 1) early stage, 2) differentiation stage representing the flagella, intercentriolar body, basal body, striated rootlets, and electron dense nucleus of thread-like lamellar configuration, and 3) growing spermatid flagella. Detailed ultrastructure of 2 different types of spermatozoa was also shown in this study.

Keywords

References

  1. Procop GW. North American paragonimiasis (caused by Paragonimus kellicotti) in the context of global paragonimiais. Clin Microbiol Rev 2009; 22: 415-446. https://doi.org/10.1128/CMR.00005-08
  2. Miyazaki I. Lung flukes in the world: morphology and life history. In Symposium on epidemiology of parasitic disease. Tokyo, Japan. International Medical Foundation of Japan. 1974, p 101-134.
  3. Doanh PN, Le NT, Tat D. Paragonimus and paragonimiasis in Vietnam. In Arizono N, Chai JY, Nawa Y, Takahashi Y, eds, Asian Parasitology Vol. 1. Food-borne helminthiasis in Asia. Chiba, Japan. Federation of Asian Parasitologists. 2005, p 149-153.
  4. Singh TS, Sugiyama H, Rangsiruji A. Paragonimus and paragonimiasis in India: review article. Indian J Med Res 2012; 136: 192-204.
  5. Vélez I, Velásquez LE, Vélez ID. Morphological description and life cycle of Paragonimus sp. (Trematoda: Troglotrematidae): causal agent of human paragonimiasis in Columbia. J Parasitol 2003; 89: 749-755. https://doi.org/10.1645/GE-2858
  6. Guan XH. Paragonimiasis. In Li YL, Guan XH, eds, Human Parasitology. Beijing, China. People's Public Health Press. 2005, p 109-113.
  7. Justine JL. Phylogeny of parasitic Platyhelminthes: a critical study of synapomorphies proposed on the basis of the ultrastructure of spermiogenesis and spermatozoa. Can J Zool 1991; 69: 1421-1440. https://doi.org/10.1139/z91-203
  8. Rohde K, Watson NA, Cribb T. Ultrastructure of sperm and spermatogenesis of Lobatostoma manteri (Trematoda, Aspidogastrea). Int J Parasitol 1991; 21: 409-419. https://doi.org/10.1016/0020-7519(91)90098-R
  9. Justine JL. Spermatozoal ultrastructure and phylogeny in the parasitic Platyhelminthes. Mèm Mus Natn Hist Nat 1995; 166: 55-86.
  10. Hathaway MA, Hathaway RP, Kritsky DC. Spermatogenesis in Octomacrum lanceatum (Monogenoidea, Oligonchoinea, Mazocraeidea). Int J Parasitol 1995; 25: 913-922. https://doi.org/10.1016/0020-7519(95)98930-A
  11. Diniz Baptista-Farias MF, Kohn A, Cohen SC. Spermatogenesis and spermiogenesis in Microcotyle sp. (Microcotylidae, Monogenea). J Parasitol 1999; 85: 832-838. https://doi.org/10.2307/3285818
  12. Agostini S, Miquel J, Ndiaye PI, Marchand B. Dicrocoelium hospes Looss, 1907 (Digenea, Dicrocoeliidae): spermiogenesis, mature spermatozoon and ultrastructural comparative study. Parasitol Res 2005; 96:38-48. https://doi.org/10.1007/s00436-005-1318-6
  13. Seck MT, Marchand B, Bâ CT. Ultrastructure of spermiogenesis and the spermatozoon of Paramphistomum microbothrium [Fischoeder 1901] (Digenea: Paramphistomidae), a parasite of Bos taurus in Senegal. Parasitol Res 2007; 101:653-662. https://doi.org/10.1007/s00436-007-0532-9
  14. Quilichini Y, Foata J, Marchand B. Ultrastructural study of the spermatozoon of Pronoprymna ventricosa (Digenea, Baccigerinae), parasite of the twaite shad Alosa fallax Lacepede (Pisces, Teleostei). Parasitol Res 2007; 101: 1125-1130. https://doi.org/10.1007/s00436-007-0599-3
  15. Hendow HT, James BL. Ultrastructure of spermatozoon and spermatogenesis in Maritrema linguilla (Digenea: Microphallidae). Int J Parasitol 1988; 18: 53-63. https://doi.org/10.1016/0020-7519(88)90036-7
  16. Orido Y, Hata H. Cytological and cytofluorometrical studies on gametogenesis of Paragonimus ohirai (Trematoda: Troglotrematidae). Int J Parasitol 1988; 18: 95-101. https://doi.org/10.1016/0020-7519(88)90042-2
  17. Watson NA, Rohde K. Re-examination of spermatogenesis of Multicotyle purvisi (Platyhelminthes, Aspidogastrea). Int J Parasitol 1995; 25: 579-586. https://doi.org/10.1016/0020-7519(94)00170-S
  18. Baptista-Farias MFD, Kohn A, Cohen SC. Ultrastructure of spermatogenesis and sperm development in Saccocoelioides godoyi Kohn & Froes, 1986 (Digenea, Haploporidae). Mem Inst Oswaldo Cruz 2001; 96: 61-70. https://doi.org/10.1590/S0074-02762001000100007
  19. Sato M, Oh M, Sakoda K. Electron microscopic study of spermatogenesis in the lung fluke (Paragonimus miyazakii). Zeitschrift für Zellforschung 1967; 77: 232-243. https://doi.org/10.1007/BF00340790
  20. Hirai H, Tada I. Morphological features of spermatozoa of Paragonimus ohirai (Trematoda: Platyhelminthes) examined by a silver nitrate staining technique. Parasitology 1991; 103: 103-110. https://doi.org/10.1017/S0031182000059345
  21. Fujino T, Ishii Y. Ultrastructural studies on spermatogenesis in a parthenogenetic type of Paragonimus westermani (Kerbert 1878) proposed as P. pulmonalis (Baelz 1880). J Parasitol 1982; 68: 433-441. https://doi.org/10.2307/3280954
  22. Orido Y. Ultrastructure of spermatozoa of the lung fluke, Paragonimus ohirai (Trematoda: Troglotrematidae), in the seminal receptacle. J Morphol 1988; 196: 333-343. https://doi.org/10.1002/jmor.1051960306
  23. Kacem H, Bakhoum AJ, Eira C, Neifar L, Miquel J. Ultrastructural characters of the spermatozoon of the digenean Hypocreadium caputvadum Kacem et al., 2011 (Lepocreadioidea: Lepocreadiidae), an intestinal parasite of Balistes capriscus in Tunisia. C R Biol 2012; 335: 637-644. https://doi.org/10.1016/j.crvi.2012.10.002
  24. Stitt AW, Fairweather I. Spermatogenesis and the fine structure of the mature spermatozoon of the liver fluke, Fasciola hepatica (Trematoda: Digenea). Parasitology 1990; 101: 395-407. https://doi.org/10.1017/S0031182000060595