DOI QR코드

DOI QR Code

Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis

  • Kongklieng, Amornmas (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Kaewkong, Worasak (Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University) ;
  • Intapan, Pewpan M. (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Sanpool, Oranuch (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Janwan, Penchom (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Thanchomnang, Tongjit (Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University) ;
  • Lulitanond, Viraphong (Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University) ;
  • Sri-Aroon, Pusadee (Applied Malacology Center, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University) ;
  • Limpanont, Yanin (Applied Malacology Center, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University) ;
  • Maleewong, Wanchai (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
  • 투고 : 2013.05.30
  • 심사 : 2013.10.11
  • 발행 : 2013.12.31

초록

Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were $84.5{\pm}0.07^{\circ}C$ and $85.7{\pm}0.07^{\circ}C$, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts.

키워드

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피인용 문헌

  1. Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis vol.16, pp.7, 2013, https://doi.org/10.3390/ijms160716085
  2. Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis vol.114, pp.11, 2013, https://doi.org/10.1007/s00436-015-4660-3
  3. Molecular characterization and functional analysis of the Schistosoma mekongi Ca2+-dependent cysteine protease (calpain) vol.12, pp.1, 2013, https://doi.org/10.1186/s13071-019-3639-9
  4. Establishment and application of a novel fluorescence-based analytical method for the rapid detection of viable bacteria in different samples vol.12, pp.31, 2013, https://doi.org/10.1039/d0ay01247e
  5. Development of a novel real-time polymerase chain reaction assay for the sensitive detection of Schistosoma japonicum in human stool vol.15, pp.10, 2013, https://doi.org/10.1371/journal.pntd.0009877