Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720

  • Intapan, Pewpan M. (Department of Parasitology and, Faculty of Medicine, Khon Kaen University) ;
  • Hirunpetcharat, Chakrit (Department of Microbiology, Faculty of Public Health, Mahidol University) ;
  • Kularbkaew, Churairat (Department of Pathology, Faculty of Medicine, Khon Kaen University) ;
  • Yutanawiboonchai, Wiboonchai (Department of Pathology, Faculty of Medicine, Khon Kaen University) ;
  • Janwan, Penchom (Department of Parasitology and, Faculty of Medicine, Khon Kaen University) ;
  • Maleewong, Wanchai (Department of Parasitology and, Faculty of Medicine, Khon Kaen University)
  • Received : 2013.05.30
  • Accepted : 2013.10.11
  • Published : 2013.12.31
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Abstract

This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.

Keywords

References

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