Parasites, Hosts and Diseases

  • 제51권1호
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  • Pages.133-137
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  • 2013
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii

  • Paim, Francine C. (Laboratory of Veterinary Clinical Analysis-LACVet, Universidade Federal de Santa Maria) ;
  • Da Silvaz, Aleksandro S. (Department of Animal Science, Universidade do Estado de Santa Catarina) ;
  • Paim, Carlos Breno V. (Laboratory of Veterinary Clinical Analysis-LACVet, Universidade Federal de Santa Maria) ;
  • Franca, Raqueli T. (Laboratory of Veterinary Clinical Analysis-LACVet, Universidade Federal de Santa Maria) ;
  • Costa, Marcio M. (Laboratory of Veterinary Clinical Analysis-LACVet, Universidade Federal de Santa Maria) ;
  • Duarte, Marta M.M.F. (Lutheran University of Brazil-ULBRA) ;
  • Sangoi, Manuela B. (Department of Clinical and Toxicological Analysis, Universidade Federal de Santa Maria) ;
  • Moresco, Rafael N. (Department of Clinical and Toxicological Analysis, Universidade Federal de Santa Maria) ;
  • Monteiro, Silvia G. (Department of Microbiology and Parasitology-LAPAVET, Universidade Federal de Santa Maria) ;
  • Lopes, Sonia Terezinha A. (Laboratory of Veterinary Clinical Analysis-LACVet, Universidade Federal de Santa Maria)
  • 투고 : 2012.04.19
  • 심사 : 2012.10.04
  • 발행 : 2013.03.15
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초록

This study aimed to measure the levels of interferon-gamma (IFN-${\gamma}$), tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate ($NO_x$) in serum of dogs experimentally infected with Rangelia vitalii. Twelve female mongrel dogs were divided into 2 groups; group A (uninfected controls) composed by healthy dogs (n=5) and group B consisting of dogs inoculated with R. vitalii (n=7). Animals were monitored by blood smear examinations, which showed intraerythrocytic forms of the parasite on day 5 post-infection (PI). Blood samples were collected through the jugular vein on days 0, 10, and 20 PI to determine the serum levels of IFN-${\gamma}$, TNF-${\alpha}$, IL-1, IL-6, and $NO_x$. Cytokines were assessed by ELISA quantitative sandwich technique, and $NO_x$ was measured by the modified Griess method. Cytokine levels (IFN-${\gamma}$, TNF-${\alpha}$, IL-1, and IL-6) were increased (P<0.01) in serum of infected animals. Serum levels of $NO_x$ were also increased on days 10 PI (P<0.01) and 20 PI (P<0.05) in infected animals. Therefore, the infection with R. vitalii causes an increase in proinflammatory cytokines and nitric oxide content. These alterations may be associated with host immune protection against the parasite.

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참고문헌

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