Parasites, Hosts and Diseases

  • 제51권2호
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  • Pages.247-253
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  • 2013
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum

  • Jia, Li-Jun (Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University) ;
  • Zhang, Shou-Fa (Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University) ;
  • Qian, Nian-Chao (Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University) ;
  • Xuan, Xue-Nan (National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine) ;
  • Yu, Long-Zheng (Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University) ;
  • Zhang, Xue-Mei (Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University) ;
  • Liu, Ming-Ming (Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University)
  • 투고 : 2012.09.26
  • 심사 : 2013.02.17
  • 발행 : 2013.04.30
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초록

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7, expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was $10^9TCID_{50}/ml$. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-${\gamma}$ and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.

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피인용 문헌

  1. Approaches for the vaccination and treatment of Neospora caninum infections in mice and ruminant models vol.143, pp.3, 2013, https://doi.org/10.1017/s0031182015001596
  2. Identification of the antigenic region of Neospora caninum dense granule protein 7 using ELISA vol.67, pp.6, 2013, https://doi.org/10.1016/j.parint.2018.06.012