Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Various Aggregate Forms of Tryptophan Synthase α-Subunit

트립토판 합성효소 α 소단위체의 다양한 단백질 덩어리 형성

  • Park, Myung Won (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
  • Lim, Woon Ki (Department of Molecular Biology, College of Natural Sciences, Pusan National University)
  • 박명원 (부산대학교 자연과학대학 분자생물학과) ;
  • 임운기 (부산대학교 자연과학대학 분자생물학과)
  • Received : 2013.01.15
  • Accepted : 2013.01.26
  • Published : 2013.02.28
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Abstract

Protein aggregation can cause diseases and hinder the production of useful recombinant proteins. The present study showed that at least three types of aggregates can be formed from tryptophan synthase ${\alpha}$-subunit (${\alpha}TS$) by varying conditions: (1) an opaque white precipitous aggregate, (2) a transparent gel-like precipitous aggregate, and (3) an unprecipitous aggregate. Macroscopically different aggregate types might suggest different mechanisms underlying aggregation processes.

단백질 덩어리는 질환의 원인이 되기도 하고, 유용한 유전자 재조합 단백질의 생산시 문제를 야기하기도 한다. 본 연구에서는 조건을 달리함으로 트립토판 합성효소 ${\alpha}$ 소단위체로부터 적어도 3가지 이상 다른 종류의 덩어리가 생길 수 있음을 보여주고 있다; (1) 불투명 흰색 침전 가능한 덩어리 (2) 투명하고 겔 유형의 침전 가능한 덩어리 (3) 불침전 덩어리. 이런 다른 종류의 덩어리 형태는 다른 기작을 통해 일어날 것으로 추정된다.

Keywords

References

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