생명과학회지 (Journal of Life Science)

  • 제23권2호
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  • Pages.311-318
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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DOI QR코드

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생리활성분획 추적방법을 통한 오미자 추출물의 항염증 활성 분석

Activity-guided Screening of Anti-inflammatory Compounds from the Hexane Extracts of Schisandra chinensis Fruit

  • 최희정 (부산대학교 의학전문대학원 해부학교실) ;
  • 최영환 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 백선용 (부산대학교 의학전문대학원 해부학교실) ;
  • 김봉선 (부산대학교 의학전문대학원 해부학교실) ;
  • 안순철 (부산대학교 의학전문대학원 미생물학 및 면역학교실) ;
  • 이문수 (한국생명공학연구원 생명자원센터) ;
  • 윤식 (부산대학교 의학전문대학원 해부학교실)
  • Choi, Hee Jung (Department of Anatomy, School of Medicine, Pusan National University) ;
  • Choi, Young-Whan (Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University) ;
  • Baek, Sun-Yong (Department of Anatomy, School of Medicine, Pusan National University) ;
  • Kim, Bong-Seon (Department of Anatomy, School of Medicine, Pusan National University) ;
  • Ahn, Soon Cheol (Department of Microbiology and Immunology, School of Medicine, Pusan National University) ;
  • Rhee, Moon-Soo (Korea Research Institute of Bioscience & Biotechnology) ;
  • Yoon, Sik (Department of Anatomy, School of Medicine, Pusan National University)
  • 투고 : 2012.01.04
  • 심사 : 2012.02.22
  • 발행 : 2013.02.28
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초록

예로부터 한방이나 민간요법에서 다양한 약리효능을 가진 약제로 알려져 있는 오미자 열매로부터 항염증 활성을 갖는 유용한 물질을 동정하고자 본 연구를 수행하였다. 먼저 오미자의 hexane 추출물로부터 38개의 분획물을 분리한 다음 각 분획의 항염증 활성을 측정하였다. 그 결과 오미자 분획물 중 생리활성이 높은 SCKH1을 선택하여 활성분획 추적방법을 통해 SCKH1PAIBPB을 분리하였다. SCKH1PAIBPB는 VCAM-1, MCP-1, IL-6 및 IL-8의 발현을 감소시키며, 혈관내피세포와 단핵구 사이의 부착능을 억제시킨다는 사실을 확인할 수 있었다. 따라서 본 연구를 통해 규명된 오미자 분획물 및 SCKH1PAIBPB의 항염증효과 뿐만 아니라 혈관내피세포 증식 및 생존능 촉진작용을 응용하여, 다양한 허혈성질환 뿐만 아니라 염중성질환의 예방 및 치료에 활용할 수 있을 것으로 사료된다.

Schisandra chinensis containing a variety of pharmacologically active lignans has been traditionally used in oriental medicine. In this study, anti-inflammatory compounds were screened from the hexane extracts of S. chinensis by activity-guided fractionation. First, we investigated the regulatory effects on the expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) with 38 fractions from the hexane extracts of S. chinensis in human umbilical vein endothelial cells (HUVECs). As a result, SCKH1 among the 38 fractions from the hexane extract of S. chinensis was selected for further analysis based on its unique regulatory effect on cell adhesion molecules, especially on VCAM-1, in LPS-stimulated HUVECs. The subsequent activity-guided fractionation of SCKH1 resulted in the purification of SCKH1PAIBPB, which was found to suppress the expression of VCAM-1, MCP-1, IL-6 and IL-8 in HUVECs stimulated with LPS, and to inhibit the adhesive capacity between HUVECs and monocytes. Taken together, our data indicate that SCKH1PAIBPB can be proposed as an effective anti-inflammatory compound that may have a potential therapeutic use for the prevention and treatment of various inflammatory diseases as well as ischemic vascular diseases.

키워드

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피인용 문헌

  1. Enhanced antimicrobial effectiveness of Omija (Schizamdra chinesis Baillon) by ClO2(chlorine dioxide) treatment vol.20, pp.6, 2013, https://doi.org/10.11002/kjfp.2013.20.6.871
  2. Antioxidation activity of residue after omija (Schisandra chinensis) juice extract vol.60, pp.2, 2017, https://doi.org/10.3839/jabc.2017.016