생명과학회지 (Journal of Life Science)

  • 제23권2호
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  • Pages.306-310
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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ELISA 방법을 이용한 요중 아플라톡신 M1 측정

Application of Competitive ELISA Method for Estimation of Urinary Aflatoxin M1 Level

  • 김용대 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 김헌 (충북대학교 의과대학 예방의학교실 및 의학연구소)
  • Kim, Yong-Dae (Department of Preventive Medicine and Medical Research Institute, College of Medicine, Chungbuk National University) ;
  • Kim, Heon (Department of Preventive Medicine and Medical Research Institute, College of Medicine, Chungbuk National University)
  • 투고 : 2013.01.02
  • 심사 : 2013.02.05
  • 발행 : 2013.02.28
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초록

본 연구는 요중 아플라톡신 M1 (AFM1)의 농도를 측정할 수 있는 competitive ELISA 방법의 특성을 HPLC-fluorescence detector (HPLC-FLD) 방법과 비교하여 평가하였다. ELISA 방법에서의 AFM1의 회수율은 105% (73-124%)였고 측정의 변이계수는 6.85%로 나타났다. ELISA 방법에서의 검출한계와 정량한계는 각각 0.20 pg/ml과 0.62 pg/ml로 조사되었으며, 두 방법을 이용하여 측정한 요중 AFM1 농도는 상관계수 0.96의 매우 높은 상관성이 있는 것으로 확인되었다(p<0.01). 그러나, 이렇게 높은 상관성에도 불구하고, ELISA 방법을 이용한 요중 AFM1의 농도는 HPLC-FLD 방법으로 측정한 값에 비해 상대적으로 높게 나타나는 경향을 보여 ELISA를 이용한 방법이 단시간에 많은 시료를 분석할 수 있는 장점은 있으나 그 결과는 HPLC-FLD 방법을 이용해서 얻은 회귀식을 이용하여 보정을 한 후 제시할 필요가 있는 것으로 판단된다.

We compared the efficacy of the competitive ELISA method for measuring the level of urinary aflatoxin M1 (AFM1) with that of the HPLC-fluorescence detector (HPLC-FLD) method. The recovery rate of AFM1 with the ELISA method was 105% (73-124%), and the coefficient of variation of the analysis was 6.85%. The ELISA method showed a 0.20 pg/ml and 0.62 pg/ml limit of detection and limit of quantitation, respectively. In correlation analysis, the two methods showed a very strong and statistically significant correlation (R=0.96, p<0.01). However, in spite of the strong correlation, the ELISA method tended to overestimate the urinary AFM1 concentration compared to the HPLC-FLD method. These results suggest that the competitive ELISA method may be a useful technique for measuring the AFM1 level in high-throughput urine samples, but it needs to be corrected with a regression equation from regression analysis with the HPLC-FLD method.

키워드

참고문헌

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피인용 문헌

  1. Current Immunoassay Methods for the Rapid Detection of Aflatoxin in Milk and Dairy Products vol.16, pp.5, 2017, https://doi.org/10.1111/1541-4337.12287