Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Micrografting and Heat Treatment Combination for Eliminating Virus of CTV-infected Citrus

CTV 바이러스 보균 감귤나무로부터 열처리와 경정접목을 통한 바이러스 제거

  • Chae, Chi Won (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yun, Su Hyun (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Jae Ho (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Hyun, Jae Wook (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Koh, Sang Wook (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Dong Hoon (Planning and Coordination Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 채치원 (국립원예특작과학원 감귤시험장) ;
  • 윤수현 (국립원예특작과학원 감귤시험장) ;
  • 박재호 (국립원예특작과학원 감귤시험장) ;
  • 현재욱 (국립원예특작과학원 감귤시험장) ;
  • 고상욱 (국립원예특작과학원 감귤시험장) ;
  • 이동훈 (국립원예특작과학원 기획조정과)
  • Received : 2013.01.14
  • Accepted : 2013.02.07
  • Published : 2013.02.28
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Abstract

This study was conducted to eliminate viruses from citrus-infected plants using micrografting and thermotherapy. Six citrus cultivars including a 'Setoka' hybrid were used as plant sources. The TAS-ELISA technique demonstrated that several plants were CTV positive. However, no CTV symptoms were detected in plants obtained from shoots and treated at a high temperature of $40^{\circ}C$ during the day and night and micrografted for two weeks with old trifoliate orange rootstock in vitro. Indexing of CTV, SDV, and CTLV for RT-PCR analysis of the eleven citrus seedlings, including 'Setoka', 'Samdajosang', 'Pungkwang', 'Shiranuhi', and 'Ehimekashi dai28go' was virus free following the micrografting and thermal therapy.

본 연구는 바이러스에 감염된 감귤나무로부터 열처리와 경정접목을 병행 처리하여 바이러스를 제거코자 수행하였다. '세토카' 교잡종을 포함한 6품종을 재료로서 사용하였으며 이를 조사된 모든 개체에서는 외관적으로 바이러스 감염 증상이 관찰되지 않았으나 TAS-ELISA 검정을 통해 전원 CTV에 감염되어 있음이 확인되었다. '세토카'를 대상으로 특정 온도 시험을 수행한 결과, 주 야간 $40^{\circ}C$ 온도 조건에서 높은 비율로 바이러스가 제거되었다. 항혈청법으로 할 수 있었으며, 그 처리에서 획득된 무균의 신초를 이용하여 기내 파종된 탱자 대목에 경정 접목하였다. 경정 접목된 모든 개체에서는 바이러스 병징이 발견되지 않았고 TAS-ELISA와 SDV 크로마토법에 의해서도 무균이 확인되었다. CTV, SDV와 CTLV를 대상으로 한 RT-PCR 검사를 통해서도 극소량의 바이러스조차 발견되지 않았다. 따라서, 열처리와 경정접목을 통해 '세토카', '삼다조생', '풍광', '부지화' 및 '에히메카시 다이28고' 품종에서 총 11개의 무균의 식물체를 획득하였다.

Keywords

References

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