생명과학회지 (Journal of Life Science)

  • 제23권2호
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  • Pages.249-258
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Cordycepin 고함유 Cordyceps militaris JLM0636 용매별 추출물의 이화학적 특성 및 항산화 효과

Antioxidative Activity and Chemical Characteristics of Cordycepin-enriched Cordyceps militaris JLM0636 Powder

  • 안희영 (동아대학교 대학원 의생명과학과) ;
  • 차재영 (대선주조(주) 기술연구소) ;
  • 정영기 (동아대학교 생명공학과) ;
  • 조영수 (동아대학교 생명공학과)
  • Ahn, Hee-Young (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Jeong, Yong-Kee (Department of Biotechnology, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, Dong-A University)
  • 투고 : 2013.01.08
  • 심사 : 2013.02.21
  • 발행 : 2013.02.28
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초록

눈꽃 동충하초(PJ), 번데기 동충하초(CM), cordycepin 고함유 번데기 동충하초($CM{\alpha}$)의 수용성, 에탄올 및 메탄올 추출물의 생리활성 물질 분석과 항산화 활성(DPPH free radical scavenging 활성, Cu/Fe-환원력, 간 조직 microsome 생체막 및 linoleic acid 과산화지질, Tyrosinase 저해활성, 혈전용해효소 활성)을 측정하였다. PJ의 수용성추출물 수율 42.53%이며 폴리페놀 화합물 함량 2.72% 및 플라보노이드 함량 1.73%로 가장 높았고 PJ, CM, $CM{\alpha}$의 주요 지방산 성분으로 palmitic acid, oleic acid, stearic acid였으며, 주요 미네랄은 K, Mg 및 Ca으로 나타났다. DPPH free radical scavenging 활성, Cu/Fe-환원력, linoleic acid의 과산화 지질 측정, Tyrosinase 저해 활성에 의한 여러 항산화 활성은 PJ가 가장 높았지만 $CM{\alpha}$가 CM보다는 항산화 활성이 높은 것을 확인했다. 또한 간 조직 microsome 생체막 지질 과산화 억제활성은 $CM{\alpha}$가 가장 활성이 좋았고 혈전 용해효소 활성에서도 $CM{\alpha}$가 높은 혈전 분해능이 있는 것을 확인하여 향후 건강기능식품이나 기능성 화장품, 혈전용해효소 개발 관련 연구의 기초자료로 활용될 수 있을 것으로 사료되어 진다.

The antioxidative activity and bioactivity of water, ethanol, and methanol extracts from Paecilomyces japonica (PJ), Cordyceps militaris (CM), and cordycepin-enriched C. militaris JLM0636 ($CM{\alpha}$) were tested in in vitro experimental models. The PJ water extract showed the highest extraction yield (42.53%). The highest content of phenolic compounds and flavonoids were found in the water extract of PJ, 2.72% and 1.73%, respectively. The major minerals were K, Mg, and Ca. The water extracts of PJ also showed the highest DPPH free radical scavenging activity and reducing power. Linoleic acid peroxidation and antioxidative activities were strong in $CM{\alpha}$. The methanol extracts of PJ showed the highest inhibition activity against tyrosinase. Fibriolytic activity was higher in $CM{\alpha}$ than in CM. These results may provide basic data to understand the biological activities of bioactive materials derived from $CM{\alpha}$ for the development of functional foods, cosmetics, and antithrombotics.

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참고문헌

  1. A.O.A.C. 1975. Official methods of analysis. 12th eds., Association of official analytical chemists. Washington, D.C., U.S.A.
  2. Astrup, T. and Mullertz, S. 1952. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys 40, 346-351. https://doi.org/10.1016/0003-9861(52)90121-5
  3. Bate-Smith, E. C. 1954. Flavonoid compounds in foods. In Advanced Food Res 5, 216.
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204.
  5. Butenas, S. and Mullertz, S. 2002. Blood coagulation. Biochemistry (Moscow) 67, 3-12. https://doi.org/10.1023/A:1013985911759
  6. Cha, J. Y. and Cho, Y. S. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J Korean Soc Food Sci Nutr 28, 1131-1136.
  7. Cha, J. Y., Kim, H. J., Chung, C. H. and Cho, Y. S. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J Korean Soc Food Sci Nutr 28, 1310-1315.
  8. Cha, J. Y., Cho, Y. S., Kim, I., Anno, T., Rahman, S. M. and Yanagita, T. 2001. Effect of hesperetin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Plant Foods Human Nutr 56, 349-358. https://doi.org/10.1023/A:1011884200848
  9. Cha, W. S., Cho, B. S. and Park, S. Y. 2004. A study on the composition of Cordyceps militaris extract and mycelium. J Life Sci 14,727-731. https://doi.org/10.5352/JLS.2004.14.5.727
  10. Cho, H. J., Cho, J. Y., Rhee, M. H., Kim, H. S., Lee, H. S. and Park, H. J. 2007. Inhibitory effects of cordycepin (3'-deoxyadenosine), a component of Cordyceps militaris, on human platelet aggregation induced by thapsigargin. J Microbiol Biotechnol 17, 1134-1138.
  11. Devasagayam, T. P., Tilak, J. C., Boloor, K. K., Sane, K. S., Sane, S. S. and Lele, R. D. 2004. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 52, 794-804.
  12. Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42. https://doi.org/10.1002/bimj.19590010102
  13. Garces, R. and Mancha, M. 1993. One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues. Anal Biochem 211, 139-143. https://doi.org/10.1006/abio.1993.1244
  14. Guo, P., Kai, Q., Gao, J., Lian, Z. Q., Wu, C. M., Wu, C. A. and Zhu, H. B. 2010. Cordycepin prevents hyperlipidemia in hamsters fed a high-fat diet via activation of AMP-activated protein kinase. J Pharmacol Sci 113, 395-403. https://doi.org/10.1254/jphs.10041FP
  15. Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoid contents in mulberry and thier scavenging effects on superoxide radicals. Food Chem 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  16. Johnson, J. E., Harma, R. D. and Miquel, J. 1986. In Free radicals, aging and degenerative disease', Alen R. Liss, N.Y.
  17. Jung, S. W., Lee, N. K., Kim, S. J. and Han, D. S. 1995. Screening of tyrosinase inhibitior from plants. Korean J Food Sci Technol 27, 891-896.
  18. Kim, E. Y., Baik, I. H., Kim, J. H., Kim, S. R. and Rhyu, M. R. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36, 333-338.
  19. Lee, J. J., Kim, A. R., Seo, Y. N. and Lee, M. Y. 2009. Comparison of physicochemical composition of three species of genus Angelica. Korean J Food Preserv 16, 94-100.
  20. Li, S. P., Zhang, G. H., Zeng, Q., Huang, Z. G., Wang, Y. T., Dong, T. T. and Tsim, K. W. 2006. Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine 13, 428-433. https://doi.org/10.1016/j.phymed.2005.02.002
  21. Masamoto, Y., Ando, H., Murata, Y., Shimoishi, Y., Tada, M. and Takahata, K. 2003. Mushroom tyrosinase inhibitory activity of esculetin isolated from seeds of Euphorbia lathyris L. Biosci Biotechnol Biochem 67, 631-634. https://doi.org/10.1271/bbb.67.631
  22. Nita, A. and Young, A. R. 2005. Melanogenesis: a photoprotective response to DNA damage. Mutation Res 571, 121-132. https://doi.org/10.1016/j.mrfmmm.2004.11.016
  23. Oh, S. L., Kim, S. S., Min, B. Y. and Chung, D. H. 1990. Composition of free sugars, free amino acids, non-volatile organic acids and tannins in the extracts of L. chinensis M., A. acutiloba K., S. chinesis B. and A. sessiliflorum S. Korean J Food Sci Technol 22, 76-81.
  24. Ohkawa, H., Ohishi, N. and Yagi, K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95, 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  25. Ok, M. and Cho, Y. S. 2005. Screening of fibrinolytic enzyme producing from microorganism in Korean fermented soybean paste and optimum conditions of enzyme production. Korean Soc Food Sci Nutr 38, 983-988.
  26. Park, C. S., Kwon, C. J., Choi, M. A., Park, G. S. and Choi, K. H. 2002. Antibacterial activities of Cordyceps spp., mugwort and pine needle extracts. Korean J Food Preserv 9, 109-113.
  27. Park, S. S., Ryu, Y. B., Lee, Y. H., Cho, Y. U., Cho, S. J., Choi, Y. J., Park, K. H. and Gal, S. W. 2007. Inhibition of melanin synthesis by mycelial culture broth of Paecilomyces japonica in the mulberry Leaf extract. J Life Sci 17, 816-821. https://doi.org/10.5352/JLS.2007.17.6.816
  28. Plaa, G. L. and Witschi, H. 1976. Chemicals, drugs and lipid peroxidation. Annu Rev Pharmacol Toxicol 16, 125-131. https://doi.org/10.1146/annurev.pa.16.040176.001013
  29. Swain, T., Hillis, W. E. and Oritega, M. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J Sci Food Agric 10, 83-88.
  30. Szolomicki, J., Samochowies, L. M., Drozdzik, W. J. and Szolomicki, S. 2000. The influence of active components of Eleutherococcus senticosus on cellular defense and physical fitness in man. Phytother Res 14, 30-35. https://doi.org/10.1002/(SICI)1099-1573(200002)14:1<30::AID-PTR543>3.0.CO;2-V
  31. Wong, S. F., Holliwell, B., Richimond, R. and Skowroneck, W. R. 1981. The role of superoxide and hydroxyl radical in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid. J Inorganic Biochem 14, 127-134. https://doi.org/10.1016/S0162-0134(00)80033-1
  32. Yun, J. S. 2009. Pathomorphological peculiarity of dometic silkworm, Bombyx mori. infected with Cordyceps, Paecilomyces tenuipes. Korean J Pesticide Sci 13, 177-184.
  33. Zhu, Q. Y., Hackman, R. M., Ensunsa, J. L., Holt, R. R. and Keen, C. L. 2002. Antioxidative activities of Oolong tea. J Agric Food Chem 50, 6929-6934. https://doi.org/10.1021/jf0206163

피인용 문헌

  1. Anti-oxidant and Anti-proliferative Effects of Water Extract Mixture of Cordyceps Militaris and Allium Tuberosum vol.26, pp.7, 2016, https://doi.org/10.5352/JLS.2016.26.7.805