Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characterization of Acute Hepatitis Virus A Genotype in Korea

국내 급성 A형 간염 바이러스의 유전자형 특징

  • Kim, Mi Hyun (Department of Clinical Pathology, Hankook Hospital) ;
  • Choi, Hayana (Department of Biology, College of Bio-Industry Science, Sunchon National University) ;
  • Pak, Kun Sik (Department of Biology, College of Bio-Industry Science, Sunchon National University) ;
  • Seong, Chi Nam (Department of Biology, College of Bio-Industry Science, Sunchon National University) ;
  • Cho, Hyun Wook (Department of Biology, College of Bio-Industry Science, Sunchon National University)
  • 김미현 (광주 한국병원 임상병리실) ;
  • 최하야나 (순천대학교 생명산업과학대학 생물학과) ;
  • 백근식 (순천대학교 생명산업과학대학 생물학과) ;
  • 성치남 (순천대학교 생명산업과학대학 생물학과) ;
  • 조현욱 (순천대학교 생명산업과학대학 생물학과)
  • Received : 2012.11.01
  • Accepted : 2013.01.21
  • Published : 2013.02.28
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Abstract

In Korea, most hepatitis A virus is the IA genotype, but reports of other genotypes have increased recently. Therefore, the purpose of this study is to conduct a genotypic analysis of acute hepatitis A virus. From April 2010 to April 2011, clinical specimens from 20 patients hospitalized with acute hepatitis A and 36 sera positive for anti-HAV IgM were obtained, and the genotype of the VP1/P2A region was analyzed. RNA sequences of the VP1/P2A junction region were amplified using RT-PCR, and the sequences were compared. From 50 sequences amplified, 4 sequences (8%) belonged to genotype IA. The remaining 46 (92%) belonged to genotype IIIA. The results indicate that the genotype of the hepatitis A virus has changed from IA to IIIA in Korea.

우리나라에서 A형 간염바이러스는 IA 유전자형이 주된 양상이었으나 최근 일부 다른 유전자형이 점차 늘어나는 보고들이 발표되고 있다. 따라서 본 연구의 목적은 최근 우리나라에서 유행한 A형 간염의 바이러스 유전자형을 분석하고자 하였다. 2010년 4월에서 2011년 4월까지 광주광역시에 위치한 K 병원에 입원한 급성 A형 간염환자 20명의 검체와 서울에 위치한 S 검사센터에 의뢰된 Anti-HAV IgM 양성인 36개의 검체를 대상으로 하여 검사를 실시하였다. 또한 Anti-HAV IgM 양성인 56개의 검체인 serum에서 RNA를 분리한 후 VP1/P2A junction 부위에 대해서 RT-PCR을 통하여 증폭 한 후 염기서열을 분석하여 기존의 발표된 유전자형과 비교하였다. 유전자형 분석결과 바이러스가 분리된 50명에서 4명(8%)은 IA 유전자형을, 46명(92%)은 IIIA 유전자형을 보였다. 이번 연구를 통하여 우리나라에서 발생한 A형 간염환자의 유전자형 분석에서 과거와는 달리 IA 유전자형이 아닌 IIIA 유전자형으로 변하였다는 것을 확인 할 수 있었다.

Keywords

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