대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)

  • 제27권1호
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  • Pages.69-81
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  • 2013
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  • 1226-8038(pISSN)
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  • 2287-9463(eISSN)
대한한방소아과학회 (The Association of Korean Oriental Pediatrics)
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맥문동탕이 호흡기 점액의 생성 및 분비에 미치는 영향

Effect of Macmundongtang on Production and Secretion of Respiratory Mucus

  • 성현경 (동국대학교 한의과대학 소아과학교실) ;
  • 민상연 (동국대학교 한의과대학 소아과학교실) ;
  • 김장현 (동국대학교 한의과대학 소아과학교실)
  • Sung, Hyun Kyung (Department of Pediatrics, College of Oriental Medicine, Dongguk University) ;
  • Min, Sang Yeon (Department of Pediatrics, College of Oriental Medicine, Dongguk University) ;
  • Kim, Jang Hyun (Department of Pediatrics, College of Oriental Medicine, Dongguk University)
  • 투고 : 2013.01.25
  • 심사 : 2013.02.15
  • 발행 : 2013.02.28
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초록

Objectives In this study, effects of Macmundongtang (MMT) on ATP or TNF-${\alpha}$ or PMA or EGF induced MUC5AC mucin production and gene expression from human airway epithelial cells and the increase in airway epithelial mucosubstances of rats were investigated. Materials and Methods Confluent NCI-H292 cells were pretreated for 30min in the presence of MMT and treated with ATP ($200{\mu}M$) or PMA (10 ng/ml) or EGF (25 ng/ml) or TNF-${\alpha}$ (0.2 nM) for 24hrs, to assess the effect of MMT both on ATP- or PMA- or EGF- or TNF-${\alpha}$-induced MUC5AC mucin production using enzyme-linked immunosorbent assay (ELISA) and on gene expression by the same inducers using reverse transcription-polymerase chain reaction (RT-PCR). At the same time, hypersecretion of airway mucus was induced by exposure of rats to SO2 during 3 weeks. Effect of orally-administered MMT during 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats was assesed using histopathological analysis after staining the epithelial tissue with PAS-alcian blue. Possible cytotoxicity of MMT was assessed by investigating the potential damage of kidney and liver functions by measuring serum GOT/GPT activities and serum BUN concentration of rats and the body weight gain during experiment, after administering MMT orally. Results (1) MMT did not only inhibit but also increased MUC5AC mucin productions and expression levels of MUC5AC gene from NCI-H292 cells. (2) MMT did not decrease the amount of intraepithelial mucosubstances of trachea of rats. (3) MMT did not show renal and hepatic toxicities and did not affect body weight gain of rats during experiment. Conclusions The result from the present study suggests that MMT might normalize the production and gene expression of airway mucin observed in various respiratory diseases accompanied by yin-deficiency, without in vivo toxicity to liver and kidney functions after oral administration.

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