한국산업보건학회지 (Journal of Korean Society of Occupational and Environmental Hygiene)

  • 제23권4호
  • /
  • Pages.393-401
  • /
  • 2013
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  • 2384-132X(pISSN)
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  • 2289-0564(eISSN)
한국산업보건학회 (Korean Industrial Hygiene Association)
권호 표지

돈사 분진 함유 내독소 수준과 축사 작업자들의 세포면역력간 상관성 분석

Relationship between Endotoxin Level of in Swine Farm Dust and Cellular Immunity of Husbandry Workers

  • 김형아 (가톨릭대학교 의과대학 예방의학교실) ;
  • 김지연 (대구가톨릭대학교 의료생명산업대학 산업보건학과) ;
  • 신경민 (대구가톨릭대학교 의료생명산업대학 산업보건학과) ;
  • 조지훈 (대구가톨릭대학교 의료생명산업대학 산업보건학과) ;
  • ;
  • 조광호 (대구파티마병원) ;
  • 허용 (대구가톨릭대학교 의료생명산업대학 산업보건학과)
  • Kim, Hyoung Ah (Catholic University of Korea, College of Medicine Dept. Preventive Medicine) ;
  • Kim, Ji Youn (Catholic University of Daegu, College of Bio-Medical Sciences Dept. Occupational Health) ;
  • Shin, Kyeong Min (Catholic University of Daegu, College of Bio-Medical Sciences Dept. Occupational Health) ;
  • Jo, Ji Hoon (Catholic University of Daegu, College of Bio-Medical Sciences Dept. Occupational Health) ;
  • Roque, Katharine (Catholic University of Daegu, College of Bio-Medical Sciences Dept. Occupational Health) ;
  • Jo, Gwang Ho (Daegu Fatima Hospital) ;
  • Heo, Yong (Catholic University of Daegu, College of Bio-Medical Sciences Dept. Occupational Health)
  • 투고 : 2013.11.01
  • 심사 : 2013.12.12
  • 발행 : 2013.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: Endotoxins in dust generated in occupational settings is known to contribute to the occurrence of respiratory illness among workers. The relationship between the level of endotoxins in total dust or respirable particulates collected from swine farms and immunological markers related with respiratory allergy was evaluated among swine husbandry workers. Materials and Methods: Peripheral blood samples were collected from ten workers at ten swine farms at Gyeonggi province, Korea. Peripheral mononuclear cells were stimulated with phorbol 12-myristate 13-acetate and ionomycin for 48 hours. The levels of various cytokines produced at culture supernatants were determined using a commercially available ELISA kit. The concentration of particulate matter($PM_{10}$) in the indoor air of the swine farms was evaluated using a PVC membrane filter and mini volume air sampler, and endotoxin levels in the dust were measured by Limulus Amebocyte Lysate Kinetic QCL method. Results: Levels of endotoxins in the total dust were categorized into high(geometric mean: $109.35EU/m^3$) and low concentrations (geometric mean: $0.95EU/m^3$) for five swine farms. Interleukin-4 levels were higher in the high endotoxin group than in the low endotoxin group, while interferon-${\gamma}$ levels were lower in the high endotoxin group than in the low endotoxin group. The ratio (interferon-${\gamma}$ to interleukin-4), indicating immunologic skewedness against allergic reactivities, was lower in the high endotoxin group($1.15{\pm}0.60$) than the low endotoxin group($3.09{\pm}2.38$). In addition, the level of interleukin-13, another cytokine contributing to the occurrence of allergic responses, was significantly higher in the at the high endotoxin group($1.12{\pm}0.37ng/m{\ell}$) than in the low endotoxin group($0.37{\pm}0.04ng/m{\ell}$). Hematologic assessment showed significantly lower cellularity in the number of total leukocytes, neutrophils, and eosinophils in the high endotoxin group than in the low endotoxin group. Conclusions: Even though a sufficient number of swine workers and farms were not investigated, this study generlly suggests that the immunological function of swine farm workers exposed to high levels of endotoxin could be modulated toward allergic reactivities.

키워드

과제정보

연구 과제 주관 기관 : 농촌진흥청

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