Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
권호 표지

Analysis of Asbestos Fiber in Lungs by Transmission Electron Microscopy According to the Digestion Method

회화방법에 따른 폐 내 석면섬유의 투과전자현미경 분석

  • Han, Jeong Hee (Occupational Safety and Health Research Institute(OSHRI), Korea Occupational Safety and Health Agency(KOSHA)) ;
  • Chung, Young Hyun (Occupational Safety and Health Research Institute(OSHRI), Korea Occupational Safety and Health Agency(KOSHA)) ;
  • Yang, Jung Sun (Occupational Safety and Health Research Institute(OSHRI), Korea Occupational Safety and Health Agency(KOSHA))
  • 한정희 (한국산업안전보건공단 산업안전보건연구원) ;
  • 정용현 (한국산업안전보건공단 산업안전보건연구원) ;
  • 양정선 (한국산업안전보건공단 산업안전보건연구원)
  • Received : 2013.09.25
  • Accepted : 2013.12.17
  • Published : 2013.12.31
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Abstract

Objectives: This study was designed to establish an accurate analytical method for asbestos in a biological sample for determining occupational asbestos-related diseases and relief of the health effects of environmental asbestos. Methods: Biological samples were obtained from lungs of rats following intratracheal instillation of asbestos(Chrysotile, anthophyllite) and were prepared according to digestion method(Wet digestion, high temperature ashing, low temperature ashing). The samples were then analyzed for asbestos fibers using a transmission electron microscope equipped with an energy dispersive X-ray spectrometer. Results: Low temperature plasma ashing removed more of the organic components and reduced fiber loss compared to the wet digestion method, making specimens so prepared more suitable for transmission electron microsocpy. Conclusions: The low temperature ashing technique is the most accurate method for analyzing asbestos in biological samples.

Keywords

References

  1. Asbestos, asbestosis, and cancer: Helsinki criteria for diagnosis and attribution. Scand J Work Environ Health 1997;23:311-316 https://doi.org/10.5271/sjweh.226
  2. Attanoos RL, Gibbs AR. Analytical techniques in diagnostic pulmonary pathology. Current Diagnostic Pathology 2004;10:252-258 https://doi.org/10.1016/j.cdip.2004.02.001
  3. Brain JD, Knudson DE, Sorokin SP, Davis MA. Pulmonary distribution of particles given by intratracheal instillation or by aerosol inhalation. Environ Res 1976;11(1):13-33 https://doi.org/10.1016/0013-9351(76)90107-9
  4. Brochard P, Monsó E, Gibbs A, Pairon JC, Dumortier P. Definitions of control populations and reference levels. Eur Respir J 1998;11:1417-1418
  5. Churg A, Wiggs B, Depaoli L, Kampe B, Stevens B. Lung asbestos content in chrysotile workers with mesothelioma. Am Rev Respir Dis 1984;130(6): 1042-1045
  6. DeVuyst P, Brochard P, Gibbs A, Fischer M, Karjalainen A, Costabel U. Basis for the interpretation of fibres and asbestos bodies in biological samples. Eur Respir J 1998;11:1423-1424
  7. DeVuyst P, Karjalainen A, Dumortier P, Pairon JC, Monso E et al., Guidelines for mineral fibre analyses in biological samples : report of the ERS working group. European Respiratory Journal 1998;11: 1416-1426 https://doi.org/10.1183/09031936.98.11061416
  8. DeVuyst P, Missouni A, Van Muylem A, Rocmans P, Dumortier P. Systematic asbestos bodies counting in lung specimens resected for lung cancer. Eur Respir J 1997;10:(Suppl. 25), 19s
  9. Gylseth B, Baunan RH, Bruun R. Analysis of inorganic fiber concentrations in biological samples by scanning electron microscopy. Scand j work environ health 1981;7:101-108 https://doi.org/10.5271/sjweh.2562
  10. Johnston I, Britton J, Kinnear W, Logan R. Rising mortality from cryptogenic fibrosing alveolitis. Br Med J 1990;301:1015-1017 https://doi.org/10.1136/bmj.301.6759.1015
  11. Kayser K, Becker C, Seeberg N, Gabius HJ. Quantitation of asbestos and asbestos-like fibers in human lung tissue by hot and wet ashing, and the significance of their presence for survival of lung carcinoma and mesothelioma patients. Lung Cancer 1999;24:89-98 https://doi.org/10.1016/S0169-5002(99)00035-5
  12. Kim SG. Compensation and diagnosis of asbestos related disease. Korean J Fam Med 2009;30:335-343 https://doi.org/10.4082/kjfm.2009.30.5.335
  13. Ministry of employment and labor(MoEL). Enforcement decree of the industrial accident compensation insurance act. 2009
  14. Ministry of environment(MoE). Enforcement decree of the asbestos injury relief act. 2011
  15. Pott F. Problems in defining carcinogenic fibers. Ann Occup Hyg 1987;31(4B):799-802 https://doi.org/10.1093/annhyg/31.4B.799
  16. Pott F. Some aspects on the dosimetry of the carcinogenic potency of asbestos and other fibrous dusts [in German]. Staub-Reinhalt Luft 1978;38:486-490
  17. Roggli VL, Gibbs AR, Attanoos R, Churg A, Popper H et al. Pathology of asbestosis-An update of the diagnostic criteria. Arch Pathol Lab Med 2010:134: 462-480
  18. Roggli VL, Greenberg SD, McLarty JW, Hurst GA, Hieger LR et al. Comparison of sputum and lung asbestos body counts in former asbestos workers. Am Rev Respir Dis 1980;122(6):941-945
  19. Roggli VL, Oury TD, Sporn TA, EDX. Pathology of Asbestos-Associated Diseases. 2nd ed. New York, NY: Springer;2004:34-70
  20. Sakai K, Hisansga N, Kojima A, Takeuchi Y Elemental composition of asbestos fibers recovered from human lung[in Japanese]. Ann. Rep. Nagoya City Public Health Res. Inst 1991;37:116-119
  21. Stanton MF, Layard M, Tegeris A, et al. Relation of particle dimension to carcinogenicity in amphibole asbestoses and other fibrous minerals. J Natl Cancer Inst 1981;67(5):965-975
  22. Stanton MF, Layard M, Tegeris A, Miller E, May M et al. Carcinogenicity of fibrous glass: pleural response in the rat in relation to fiber dimension. J Natl Cancer Inst 1977;58(3):587-603 https://doi.org/10.1093/jnci/58.3.587
  23. Stanton MF, Wrench C. Mechanisms of mesothelioma induction with asbestos and fibrous glass. J Natl Cancer Inst 1972;48(3):797-821